Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum

Peters-Wendisch P, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ (2001)
Journal of Molecular Microbiology and Biotechnology 3(2): 295-300.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Corynebacterium glutamicum possesses both phosphoenolpyruvate carboxylase (PEPCx) and pyruvate carboxylase (PCx) as anaplerotic enzymes for growth on carbohydrates. To analyze the significance of PCx for the amino acid production by this organism, the wild-type pyc gene, encoding PCx, was used for the construction of defined pyc-inactive and pyc-overexpressing strains and the glutamate, lysine and threonine production capabilities of these recombinant strains of C. glutamicum were tested in comparison to the respective host strains. No PCx activity was observed in the pyc-inactive mutants whereas the pyc-overexpressing strains showed eight-to elevenfold higher specific PCx activity when compared to the host strains. In a detergent-dependent glutamate production assay, the pyc-overexpressing strain showed more than sevenfold higher, the PCx-deficient strain about twofold lower glutamate production than the wild-type, Overexpression of the pyc gene and thus increasing the PCx activity in a lysine-producing strain of C. glutamicum resulted in approximately 50% higher lysine accumulation in the culture supernatant whereas inactivation of the pyc gene led to a decrease by 60%, In a threonine-producing strain of C. glutamicum, the overexpression of the pyc gene led to an only 10 to 20% increase in threonine production, however, to a more than 150% increase in the production of the threonine precursor homoserine. These results identify the anaplerotic PCx reaction as a major bottleneck for amino acid production by C. glutamicum and show that the enzyme is an important target for the molecular breeding of hyperproducing strains.
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Zeitschriftentitel
Journal of Molecular Microbiology and Biotechnology
Band
3
Ausgabe
2
Seite(n)
295-300
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Peters-Wendisch P, Schiel B, Wendisch VF, et al. Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology. 2001;3(2):295-300.
Peters-Wendisch, P., Schiel, B., Wendisch, V. F., Katsoulidis, E., Mockel, B., Sahm, H., & Eikmanns, B. J. (2001). Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology, 3(2), 295-300.
Peters-Wendisch, P., Schiel, B., Wendisch, V. F., Katsoulidis, E., Mockel, B., Sahm, H., and Eikmanns, B. J. (2001). Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology 3, 295-300.
Peters-Wendisch, P., et al., 2001. Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology, 3(2), p 295-300.
P. Peters-Wendisch, et al., “Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum”, Journal of Molecular Microbiology and Biotechnology, vol. 3, 2001, pp. 295-300.
Peters-Wendisch, P., Schiel, B., Wendisch, V.F., Katsoulidis, E., Mockel, B., Sahm, H., Eikmanns, B.J.: Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. Journal of Molecular Microbiology and Biotechnology. 3, 295-300 (2001).
Peters-Wendisch, Petra, Schiel, B., Wendisch, Volker F., Katsoulidis, E., Mockel, B., Sahm, H., and Eikmanns, B. J. “Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum”. Journal of Molecular Microbiology and Biotechnology 3.2 (2001): 295-300.

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PMID: 28256782
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PMID: 28701150
Efficient production and secretion of oxaloacetate from Halomonas sp. KM-1 under aerobic conditions.
Hannya A, Nishimura T, Matsushita I, Tsubota J, Kawata Y., AMB Express 7(1), 2017
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Expression of recombinant protein using Corynebacterium Glutamicum: progress, challenges and applications.
Liu X, Yang Y, Zhang W, Sun Y, Peng F, Jeffrey L, Harvey L, McNeil B, Bai Z., Crit Rev Biotechnol 36(4), 2016
PMID: 25714007
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PMID: 27350619
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PMID: 27376307
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PMID: 27520809
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PMID: 25431011
Fermentative production of the diamine putrescine: system metabolic engineering of corynebacterium glutamicum.
Nguyen AQ, Schneider J, Reddy GK, Wendisch VF., Metabolites 5(2), 2015
PMID: 25919117
Synthesis of chemicals by metabolic engineering of microbes.
Sun X, Shen X, Jain R, Lin Y, Wang J, Sun J, Wang J, Yan Y, Yuan Q., Chem Soc Rev 44(11), 2015
PMID: 25940754
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Leßmeier L, Pfeifenschneider J, Carnicer M, Heux S, Portais JC, Wendisch VF., Appl Microbiol Biotechnol 99(23), 2015
PMID: 26276544
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PMID: 24270893
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Chen Z, Bommareddy RR, Frank D, Rappert S, Zeng AP., Appl Environ Microbiol 80(4), 2014
PMID: 24334667
Reducing lactate secretion by ldhA Deletion in L-glutamate- producing strain Corynebacterium glutamicum GDK-9.
Zhang D, Guan D, Liang J, Guo C, Xie X, Zhang C, Xu Q, Chen N., Braz J Microbiol 45(4), 2014
PMID: 25763057
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PMID: 24486440
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PMID: 24737727
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PMID: 25191655
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PMID: 25181035
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PMID: 23447448
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Chen X, Xu G, Xu N, Zou W, Zhu P, Liu L, Chen J., Metab Eng 19(), 2013
PMID: 23707987
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PMID: 23725060
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PMID: 23806148
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PMID: 24034837
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Litsanov B, Kabus A, Brocker M, Bott M., Microb Biotechnol 5(1), 2012
PMID: 22018023
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PMID: 22159614
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PMID: 22243621
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van Ooyen J, Noack S, Bott M, Reth A, Eggeling L., Biotechnol Bioeng 109(8), 2012
PMID: 22392073
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PMID: 22963379
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Choorapoikayil S, Schoepe J, Buchinger S, Schomburg D., OMICS 16(12), 2012
PMID: 23215805
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Becker J, Zelder O, Häfner S, Schröder H, Wittmann C., Metab Eng 13(2), 2011
PMID: 21241816
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Lindner SN, Seibold GM, Henrich A, Krämer R, Wendisch VF., Appl Environ Microbiol 77(11), 2011
PMID: 21478323
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Nešvera J, Pátek M., Appl Microbiol Biotechnol 90(5), 2011
PMID: 21519933
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Schneider J, Wendisch VF., Appl Microbiol Biotechnol 91(1), 2011
PMID: 21552989
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Nærdal I, Netzer R, Ellingsen TE, Brautaset T., Appl Environ Microbiol 77(17), 2011
PMID: 21724876
Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum.
Gopinath V, Meiswinkel TM, Wendisch VF, Nampoothiri KM., Appl Microbiol Biotechnol 92(5), 2011
PMID: 21796382
Carboxylases in natural and synthetic microbial pathways.
Erb TJ., Appl Environ Microbiol 77(24), 2011
PMID: 22003013
Inhibitors of Pyruvate Carboxylase.
Zeczycki TN, Maurice MS, Attwood PV., Open Enzym Inhib J 3(), 2010
PMID: 22180764
Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for L-lysine production from methanol at 50 degrees C.
Brautaset T, Jakobsen ØM, Degnes KF, Netzer R, Naerdal I, Krog A, Dillingham R, Flickinger MC, Ellingsen TE., Appl Microbiol Biotechnol 87(3), 2010
PMID: 20372887
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Schneider J, Wendisch VF., Appl Microbiol Biotechnol 88(4), 2010
PMID: 20661733
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Kohlstedt M, Becker J, Wittmann C., Appl Microbiol Biotechnol 88(5), 2010
PMID: 20821203
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Kato O, Youn JW, Stansen KC, Matsui D, Oikawa T, Wendisch VF., BMC Microbiol 10(), 2010
PMID: 21159175
Acetohydroxyacid synthase, a novel target for improvement of L-lysine production by Corynebacterium glutamicum.
Blombach B, Hans S, Bathe B, Eikmanns BJ., Appl Environ Microbiol 75(2), 2009
PMID: 19047397
Expression and localization of the Corynebacterium glutamicum NCgl1221 protein encoding an L-glutamic acid exporter.
Yao W, Deng X, Liu M, Zheng P, Sun Z, Zhang Y., Microbiol Res 164(6), 2009
PMID: 19233628
Exopolyphosphatases PPX1 and PPX2 from Corynebacterium glutamicum.
Lindner SN, Knebel S, Wesseling H, Schoberth SM, Wendisch VF., Appl Environ Microbiol 75(10), 2009
PMID: 19304823
Double deletion of dtsR1 and pyc induce efficient L: -glutamate overproduction in Corynebacterium glutamicum.
Yao W, Deng X, Zhong H, Liu M, Zheng P, Sun Z, Zhang Y., J Ind Microbiol Biotechnol 36(7), 2009
PMID: 19408028
Characterization of the dicarboxylate transporter DctA in Corynebacterium glutamicum.
Youn JW, Jolkver E, Krämer R, Marin K, Wendisch VF., J Bacteriol 191(17), 2009
PMID: 19581365
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Becker J, Klopprogge C, Schröder H, Wittmann C., Appl Environ Microbiol 75(24), 2009
PMID: 19820141
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Hasegawa T, Hashimoto K, Kawasaki H, Nakamatsu T., J Biosci Bioeng 105(1), 2008
PMID: 18295714
Corynebacterium glutamicum tailored for high-yield L-valine production.
Blombach B, Schreiner ME, Bartek T, Oldiges M, Eikmanns BJ., Appl Microbiol Biotechnol 79(3), 2008
PMID: 18379776
Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum.
Sato H, Orishimo K, Shirai T, Hirasawa T, Nagahisa K, Shimizu H, Wachi M., J Biosci Bioeng 106(1), 2008
PMID: 18691531
Identification and characterization of the dicarboxylate uptake system DccT in Corynebacterium glutamicum.
Youn JW, Jolkver E, Krämer R, Marin K, Wendisch VF., J Bacteriol 190(19), 2008
PMID: 18658264
Engineering of a glycerol utilization pathway for amino acid production by Corynebacterium glutamicum.
Rittmann D, Lindner SN, Wendisch VF., Appl Environ Microbiol 74(20), 2008
PMID: 18757581
Engineering primary metabolic pathways of industrial micro-organisms.
Kern A, Tilley E, Hunter IS, Legisa M, Glieder A., J Biotechnol 129(1), 2007
PMID: 17196287
Bacillus methanolicus: a candidate for industrial production of amino acids from methanol at 50 degrees C.
Brautaset T, Jakobsen ØM, Josefsen KD, Flickinger MC, Ellingsen TE., Appl Microbiol Biotechnol 74(1), 2007
PMID: 17216461
Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicum.
Blombach B, Schreiner ME, Moch M, Oldiges M, Eikmanns BJ., Appl Microbiol Biotechnol 76(3), 2007
PMID: 17333167
The two carboxylases of Corynebacterium glutamicum essential for fatty acid and mycolic acid synthesis.
Gande R, Dover LG, Krumbach K, Besra GS, Sahm H, Oikawa T, Eggeling L., J Bacteriol 189(14), 2007
PMID: 17483212
Characteristics of methionine production by an engineered Corynebacterium glutamicum strain.
Park SD, Lee JY, Sim SY, Kim Y, Lee HS., Metab Eng 9(4), 2007
PMID: 17604670
NCgl2620 encodes a class II polyphosphate kinase in Corynebacterium glutamicum.
Lindner SN, Vidaurre D, Willbold S, Schoberth SM, Wendisch VF., Appl Environ Microbiol 73(15), 2007
PMID: 17545325
Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum--over expression and modification of G6P dehydrogenase.
Becker J, Klopprogge C, Herold A, Zelder O, Bolten CJ, Wittmann C., J Biotechnol 132(2), 2007
PMID: 17624457
Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis.
Shirai T, Fujimura K, Furusawa C, Nagahisa K, Shioya S, Shimizu H., Microb Cell Fact 6(), 2007
PMID: 17587457
Prospects for a bio-based succinate industry.
McKinlay JB, Vieille C, Zeikus JG., Appl Microbiol Biotechnol 76(4), 2007
PMID: 17609945
Emerging Corynebacterium glutamicum systems biology.
Wendisch VF, Bott M, Kalinowski J, Oldiges M, Wiechert W., J Biotechnol 124(1), 2006
PMID: 16406159
Enhanced glutamic acid production by a H+-ATPase-defective mutant of Corynebacterium glutamicum.
Aoki R, Wada M, Takesue N, Tanaka K, Yokota A., Biosci Biotechnol Biochem 69(8), 2005
PMID: 16116273
Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production.
Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF., Appl Environ Microbiol 71(10), 2005
PMID: 16204505
Metabolic engineering of Corynebacterium glutamicum for L-serine production.
Peters-Wendisch P, Stolz M, Etterich H, Kennerknecht N, Sahm H, Eggeling L., Appl Environ Microbiol 71(11), 2005
PMID: 16269752
Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions.
Inui M, Murakami S, Okino S, Kawaguchi H, Vertès AA, Yukawa H., J Mol Microbiol Biotechnol 7(4), 2004
PMID: 15383716
Glutamate as an inhibitor of phosphoenolpyruvate carboxylase activity in Corynebacterium glutamicum.
Delaunay S, Daran-Lapujade P, Engasser JM, Goergen JL., J Ind Microbiol Biotechnol 31(4), 2004
PMID: 15133716
Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis.
Netzer R, Krause M, Rittmann D, Peters-Wendisch PG, Eggeling L, Wendisch VF, Sahm H., Arch Microbiol 182(5), 2004
PMID: 15375646
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegräbe I, Tauch A., J Biotechnol 104(1-3), 2003
PMID: 12948626
Acetate metabolism and its regulation in Corynebacterium glutamicum.
Gerstmeir R, Wendisch VF, Schnicke S, Ruan H, Farwick M, Reinscheid D, Eikmanns BJ., J Biotechnol 104(1-3), 2003
PMID: 12948633
The use of enzymes in the chemical industry in Europe.
Schmid A, Hollmann F, Park JB, Bühler B., Curr Opin Biotechnol 13(4), 2002
PMID: 12323359
IGF-I promotes a shift in metabolic flux in vascular smooth muscle cells.
Hall JL, Gibbons GH, Chatham JC., Am J Physiol Endocrinol Metab 283(3), 2002
PMID: 12169439

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