Formation of volutin granules in Corynebacterium glutamicum

Pallerla SR, Knebel S, Polen T, Klauth P, Hollender J, Wendisch VF, Schoberth SM (2005)
Fems Microbiology Letters 243(1): 133-140.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Volutin granules are intracellular storages of complexed inorganic polyphosphate (poly P). Histochemical staining procedures differentiate between pathogenic corynebacteria such as Corynebacterum diphtheriae (containing volutin) and non-pathogenic species. such as C. glutamicum. Here we report that strains ATCC13032 and MH20-22B of the non-pathogenic C. glutamicum also formed subcellular entities (18-37% of the total cell volume) that had the typical characteristics of volutin granules: (i) volutin staining (ii) green UV fluorescence when stained with 4',6-diamidino-2-phenylindole, (iii) electron-dense and rich in phosphorus when determined with transmission electron microscopy and X-ray microanalysis, and (iv) P-31 NMR poly P resonances of isolated granules dissolved in EDTA. MgCl2 addition to the growth medium stimulated granule formation but did not effect expression of genes involved in poly P metabolism. Granular volutin fractions from lysed cells contained polyphosphate glucokinase as detected by SDS-PAGE/MALDI-TOF, indicating that this poly P metabolizing enzyme is present also in intact poly P granules. The results suggest that formation of volutin is a more widespread phenomenon than generally accepted. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Stichworte
polyphosphate; bacteria; expression; culture; strain; cells; nmr; DNA microarray; micrococcus-lysodeikticus; maldi-tof; polyphosphate glucokinase; p-31 nmr; magnesium; dapi fluorescence; electron dispersive x-ray analysis; corynebacterium glutamicum; volutin granules; inorganic polyphosphate
Erscheinungsjahr
2005
Zeitschriftentitel
Fems Microbiology Letters
Band
243
Ausgabe
1
Seite(n)
133-140
ISSN
0378-1097
eISSN
1574-6968
Page URI
https://pub.uni-bielefeld.de/record/1895170

Zitieren

Pallerla SR, Knebel S, Polen T, et al. Formation of volutin granules in Corynebacterium glutamicum. Fems Microbiology Letters. 2005;243(1):133-140.
Pallerla, S. R., Knebel, S., Polen, T., Klauth, P., Hollender, J., Wendisch, V. F., & Schoberth, S. M. (2005). Formation of volutin granules in Corynebacterium glutamicum. Fems Microbiology Letters, 243(1), 133-140. doi:10.1016/j.femsle.2004.11.047
Pallerla, S. R., Knebel, S., Polen, T., Klauth, P., Hollender, J., Wendisch, V. F., and Schoberth, S. M. (2005). Formation of volutin granules in Corynebacterium glutamicum. Fems Microbiology Letters 243, 133-140.
Pallerla, S.R., et al., 2005. Formation of volutin granules in Corynebacterium glutamicum. Fems Microbiology Letters, 243(1), p 133-140.
S.R. Pallerla, et al., “Formation of volutin granules in Corynebacterium glutamicum”, Fems Microbiology Letters, vol. 243, 2005, pp. 133-140.
Pallerla, S.R., Knebel, S., Polen, T., Klauth, P., Hollender, J., Wendisch, V.F., Schoberth, S.M.: Formation of volutin granules in Corynebacterium glutamicum. Fems Microbiology Letters. 243, 133-140 (2005).
Pallerla, S. R., Knebel, S., Polen, T., Klauth, P., Hollender, J., Wendisch, Volker F., and Schoberth, S. M. “Formation of volutin granules in Corynebacterium glutamicum”. Fems Microbiology Letters 243.1 (2005): 133-140.

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