Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation

Krings E, Krumbach K, Bathe B, Kelle R, Wendisch VF, Sahm H, Eggeling L (2006)
Journal of Bacteriology 188(23): 8054-8061.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Krings, E.; Krumbach, K.; Bathe, B.; Kelle, R.; Wendisch, Volker F.UniBi ; Sahm, H.; Eggeling, L.
Abstract / Bemerkung
Although numerous bacteria possess genes annotated iol in their genomes, there have been very few studies on the possibly associated myo-inositol metabolism and its significance for the cell. We found that Corynebacterium glutamicum utilizes myo-inositol as a carbon and energy source, enabling proliferation with a high maximum rate of 0.35 h(-1). Whole-genome DNA microarray analysis revealed that 31 genes respond to myo-inositol utilization, with 21 of them being localized in two clusters of > 14 kb. A set of genomic mutations and functional studies yielded the result that some genes in the two clusters are redundant, and only cluster I is necessary for catabolizing the polyol. There are three genes which encode carriers belonging to the major facilitator superfamily and which exhibit a > 12-fold increased mRNA level on myo-inositol. As revealed by mutant characterizations, one carrier is not involved in myo-inositol uptake whereas the other two are active and can completely replace each other with apparent K(m)s for myo-inositol as a substrate of 0.20 mM and 0.45 mM, respectively. Interestingly, upon utilization of myo-inositol, the L-lysine yield is 0.10 mol/mol, as opposed to 0.30 mol/mol, with glucose as the substrate. This is probably not only due to myo-inositol metabolism alone since a mixture of 187 mM glucose and 17 mM myo-inositol, where the polyol only contributes 8% of the total carbon, reduced the L-lysine yield by 29%. Moreover, genome comparisons with other bacteria highlight the core genes required for growth on myo-inositol, whose metabolism is still weakly defined.
Stichworte
expression; pathway; enzyme; dehydrogenase; bacillus-subtilis; escherichia-coli; mycobacterium-tuberculosis; acid; operon; glucose
Erscheinungsjahr
2006
Zeitschriftentitel
Journal of Bacteriology
Band
188
Ausgabe
23
Seite(n)
8054-8061
ISSN
0021-9193
Page URI
https://pub.uni-bielefeld.de/record/1895091

Zitieren

Krings E, Krumbach K, Bathe B, et al. Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology. 2006;188(23):8054-8061.
Krings, E., Krumbach, K., Bathe, B., Kelle, R., Wendisch, V. F., Sahm, H., & Eggeling, L. (2006). Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology, 188(23), 8054-8061. doi:10.1128/Jb.00935-06
Krings, E., Krumbach, K., Bathe, B., Kelle, R., Wendisch, V. F., Sahm, H., and Eggeling, L. (2006). Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology 188, 8054-8061.
Krings, E., et al., 2006. Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology, 188(23), p 8054-8061.
E. Krings, et al., “Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation”, Journal of Bacteriology, vol. 188, 2006, pp. 8054-8061.
Krings, E., Krumbach, K., Bathe, B., Kelle, R., Wendisch, V.F., Sahm, H., Eggeling, L.: Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. Journal of Bacteriology. 188, 8054-8061 (2006).
Krings, E., Krumbach, K., Bathe, B., Kelle, R., Wendisch, Volker F., Sahm, H., and Eggeling, L. “Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation”. Journal of Bacteriology 188.23 (2006): 8054-8061.

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