Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum

Klauth P, Pallerla SR, Vidaurre D, Ralfs C, Wendisch VF, Schoberth SM (2006)
Applied Microbiology and Biotechnology 72(5): 1099-1106.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Corynebacterium glutamicum forms inorganic polyphosphate (poly P) that may occur as soluble (cytosolic) poly P and/or as volutin granules. A suitable method for monitoring soluble and granular poly P in C. glutamicum was developed and applied to C. glutamicum cells cultivated under different growth conditions. Under phosphate-limiting conditions, C. glutamicum did not accumulate poly P, but it rebuilt its poly P storages when phosphate became available. The poly P content of C. glutamicum growing on glucose minimal medium with sufficient phosphate varied considerably during growth. While the poly P content was minimal in the midexponential growth phase, two maxima were observed in the early exponential growth phase and at entry into the stationary growth phase. Cells in the early exponential growth phase primarily contained granular poly P, while cells entering the stationary growth phase contained soluble, cytosolic poly P. These results and those obtained for C. glutamicum cells cultivated under hypo- or hyperosmotic conditions or during glutamate production revealed that the poly P content of C. glutamicum and the partitioning between cytosolic and granular forms of poly P are dynamics and depend on the growth conditions.
Stichworte
dapi; expression; operon; volutin granules; bacteria; corynebacterium glutamicum; growth; digital image analysis; glutamate production; tween 40; p-31 nmr; phosphate starvation; osmoshock; ethambutol; dapi; poly p
Erscheinungsjahr
2006
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
72
Ausgabe
5
Seite(n)
1099-1106
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/1895073

Zitieren

Klauth P, Pallerla SR, Vidaurre D, Ralfs C, Wendisch VF, Schoberth SM. Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 2006;72(5):1099-1106.
Klauth, P., Pallerla, S. R., Vidaurre, D., Ralfs, C., Wendisch, V. F., & Schoberth, S. M. (2006). Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 72(5), 1099-1106. doi:10.1007/s00253-006-0562-8
Klauth, P., Pallerla, S. R., Vidaurre, D., Ralfs, C., Wendisch, V. F., and Schoberth, S. M. (2006). Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum. Applied Microbiology and Biotechnology 72, 1099-1106.
Klauth, P., et al., 2006. Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 72(5), p 1099-1106.
P. Klauth, et al., “Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum”, Applied Microbiology and Biotechnology, vol. 72, 2006, pp. 1099-1106.
Klauth, P., Pallerla, S.R., Vidaurre, D., Ralfs, C., Wendisch, V.F., Schoberth, S.M.: Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 72, 1099-1106 (2006).
Klauth, P., Pallerla, S. R., Vidaurre, D., Ralfs, C., Wendisch, Volker F., and Schoberth, S. M. “Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum”. Applied Microbiology and Biotechnology 72.5 (2006): 1099-1106.

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