Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation
Kabus A, Georgi T, Wendisch VF, Bott M (2007)
Applied Microbiology and Biotechnology 75(1): 47-53.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Kabus, A.;
Georgi, T.;
Wendisch, Volker F.UniBi
;
Bott, M.
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Einrichtung
Abstract / Bemerkung
A critical factor in the biotechnological production of (L)-lysine with Corynebacterium glutamicum is the sufficient supply of NADPH. The membrane-integral nicotinamide nucleotide transhydrogenase PntAB of Escherichia coli can use the electrochemical proton gradient across the cytoplasmic membrane to drive the reduction of NADP(+) stop via the oxidation of NADH. As C. glutamicum does not possess such an enzyme, we expressed the E. coli pntAB genes in the genetically defined C. glutamicum lysine-producing strain DM1730, resulting in membrane-associated transhydrogenase activity of 0.7 U/mg protein. When cultivated in minimal medium with 10% (w/v) carbon source, the presence of transhydrogenase slightly reduced glucose consumption, whereas the consumption of fructose, glucose plus fructose, and, in particular, sucrose was stimulated. Biomass was increased by pntAB expression between 10 and 30% on all carbon sources tested. Most importantly, the lysine concentration was increased in the presence of transhydrogenase by similar to 10% on glucose, similar to 70% on fructose, similar to 50% on glucose plus fructose, and even by similar to 300% on sucrose. Thus, the presence of a proton-coupled transhydrogenase was shown to be an efficient way to improve lysine production by C. glutamicum. In contrast, pntAB expression had a negative effect on growth and glutamate production of C. glutamicum wild type.
Stichworte
central metabolism;
biochemical-characterization;
pntab;
nicotinamide nucleotide transhydrogenase;
sucrose;
fructose;
glucose;
glutamate production;
corynebacterium glutamicum;
lysine production;
escherichia coli;
malic enzyme;
l-glutamate;
amino-acids;
fructose;
cloning;
glucose;
fructose-1;
6-bisphosphatase;
bacteria
Erscheinungsjahr
2007
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
75
Ausgabe
1
Seite(n)
47-53
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/1895028
Zitieren
Kabus A, Georgi T, Wendisch VF, Bott M. Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation. Applied Microbiology and Biotechnology. 2007;75(1):47-53.
Kabus, A., Georgi, T., Wendisch, V. F., & Bott, M. (2007). Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation. Applied Microbiology and Biotechnology, 75(1), 47-53. https://doi.org/10.1007/s00253-006-0804-9
Kabus, A., Georgi, T., Wendisch, Volker F., and Bott, M. 2007. “Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation”. Applied Microbiology and Biotechnology 75 (1): 47-53.
Kabus, A., Georgi, T., Wendisch, V. F., and Bott, M. (2007). Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation. Applied Microbiology and Biotechnology 75, 47-53.
Kabus, A., et al., 2007. Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation. Applied Microbiology and Biotechnology, 75(1), p 47-53.
A. Kabus, et al., “Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation”, Applied Microbiology and Biotechnology, vol. 75, 2007, pp. 47-53.
Kabus, A., Georgi, T., Wendisch, V.F., Bott, M.: Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation. Applied Microbiology and Biotechnology. 75, 47-53 (2007).
Kabus, A., Georgi, T., Wendisch, Volker F., and Bott, M. “Expression of the Escherichia coli pntAB genes encoding a membrane-bound transhydrogenase in Corynebacterium glutamicum improves L-lysine formation”. Applied Microbiology and Biotechnology 75.1 (2007): 47-53.
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Daten bereitgestellt von Europe PubMed Central.
Application of leucine dehydrogenase Bcd from Bacillus subtilis for l-valine synthesis in Escherichia coli under microaerobic conditions.
Savrasova EA, Stoynova NV., Heliyon 5(4), 2019
PMID: 30993221
Savrasova EA, Stoynova NV., Heliyon 5(4), 2019
PMID: 30993221
NADPH metabolism: a survey of its theoretical characteristics and manipulation strategies in amino acid biosynthesis.
Xu JZ, Yang HK, Zhang WG., Crit Rev Biotechnol 38(7), 2018
PMID: 29480038
Xu JZ, Yang HK, Zhang WG., Crit Rev Biotechnol 38(7), 2018
PMID: 29480038
Redox cofactor engineering in industrial microorganisms: strategies, recent applications and future directions.
Liu J, Li H, Zhao G, Caiyin Q, Qiao J., J Ind Microbiol Biotechnol 45(5), 2018
PMID: 29582241
Liu J, Li H, Zhao G, Caiyin Q, Qiao J., J Ind Microbiol Biotechnol 45(5), 2018
PMID: 29582241
Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl-CoA synthetase and anaplerotic enzymes in engineered Escherichia coli.
Song HS, Seo HM, Jeon JM, Moon YM, Hong JW, Hong YG, Bhatia SK, Ahn J, Lee H, Kim W, Park YC, Choi KY, Kim YG, Yang YH., Biotechnol Bioeng 115(8), 2018
PMID: 29663332
Song HS, Seo HM, Jeon JM, Moon YM, Hong JW, Hong YG, Bhatia SK, Ahn J, Lee H, Kim W, Park YC, Choi KY, Kim YG, Yang YH., Biotechnol Bioeng 115(8), 2018
PMID: 29663332
Improving catalytic activity of the Baeyer-Villiger monooxygenase-based Escherichia coli biocatalysts for the overproduction of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid.
Woo JM, Jeon EY, Seo EJ, Seo JH, Lee DY, Yeon YJ, Park JB., Sci Rep 8(1), 2018
PMID: 29980730
Woo JM, Jeon EY, Seo EJ, Seo JH, Lee DY, Yeon YJ, Park JB., Sci Rep 8(1), 2018
PMID: 29980730
Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions.
Graf M, Zieringer J, Haas T, Nieß A, Blombach B, Takors R., Front Microbiol 9(), 2018
PMID: 30210489
Graf M, Zieringer J, Haas T, Nieß A, Blombach B, Takors R., Front Microbiol 9(), 2018
PMID: 30210489
Real Time Monitoring of NADPH Concentrations in Corynebacterium glutamicum and Escherichia coli via the Genetically Encoded Sensor mBFP.
Goldbeck O, Eck AW, Seibold GM., Front Microbiol 9(), 2018
PMID: 30405597
Goldbeck O, Eck AW, Seibold GM., Front Microbiol 9(), 2018
PMID: 30405597
NADPH-related processes studied with a SoxR-based biosensor in Escherichia coli.
Spielmann A, Baumgart M, Bott M., Microbiologyopen (), 2018
PMID: 30585443
Spielmann A, Baumgart M, Bott M., Microbiologyopen (), 2018
PMID: 30585443
Malonyl-CoA pathway: a promising route for 3-hydroxypropionate biosynthesis.
Liu C, Ding Y, Xian M, Liu M, Liu H, Ma Q, Zhao G., Crit Rev Biotechnol 37(7), 2017
PMID: 28078904
Liu C, Ding Y, Xian M, Liu M, Liu H, Ma Q, Zhao G., Crit Rev Biotechnol 37(7), 2017
PMID: 28078904
Redirecting carbon flux through pgi-deficient and heterologous transhydrogenase toward efficient succinate production in Corynebacterium glutamicum.
Wang C, Zhou Z, Cai H, Chen Z, Xu H., J Ind Microbiol Biotechnol 44(7), 2017
PMID: 28303352
Wang C, Zhou Z, Cai H, Chen Z, Xu H., J Ind Microbiol Biotechnol 44(7), 2017
PMID: 28303352
Expanding metabolic pathway for de novo biosynthesis of the chiral pharmaceutical intermediate L-pipecolic acid in Escherichia coli.
Ying H, Tao S, Wang J, Ma W, Chen K, Wang X, Ouyang P., Microb Cell Fact 16(1), 2017
PMID: 28347340
Ying H, Tao S, Wang J, Ma W, Chen K, Wang X, Ouyang P., Microb Cell Fact 16(1), 2017
PMID: 28347340
A new genome-scale metabolic model of Corynebacterium glutamicum and its application.
Zhang Y, Cai J, Shang X, Wang B, Liu S, Chai X, Tan T, Zhang Y, Wen T., Biotechnol Biofuels 10(), 2017
PMID: 28680478
Zhang Y, Cai J, Shang X, Wang B, Liu S, Chai X, Tan T, Zhang Y, Wen T., Biotechnol Biofuels 10(), 2017
PMID: 28680478
Holistic bioengineering: rewiring central metabolism for enhanced bioproduction.
Aslan S, Noor E, Bar-Even A., Biochem J 474(23), 2017
PMID: 29146872
Aslan S, Noor E, Bar-Even A., Biochem J 474(23), 2017
PMID: 29146872
Increasing available NADH supply during succinic acid production by Corynebacterium glutamicum.
Zhou Z, Wang C, Chen Y, Zhang K, Xu H, Cai H, Chen Z., Biotechnol Prog 31(1), 2015
PMID: 25311136
Zhou Z, Wang C, Chen Y, Zhang K, Xu H, Cai H, Chen Z., Biotechnol Prog 31(1), 2015
PMID: 25311136
Metabolic engineering of an ATP-neutral Embden-Meyerhof-Parnas pathway in Corynebacterium glutamicum: growth restoration by an adaptive point mutation in NADH dehydrogenase.
Komati Reddy G, Lindner SN, Wendisch VF., Appl Environ Microbiol 81(6), 2015
PMID: 25576602
Komati Reddy G, Lindner SN, Wendisch VF., Appl Environ Microbiol 81(6), 2015
PMID: 25576602
Advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products.
Becker J, Wittmann C., Angew Chem Int Ed Engl 54(11), 2015
PMID: 25684732
Becker J, Wittmann C., Angew Chem Int Ed Engl 54(11), 2015
PMID: 25684732
Synthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from unrelated carbon sources in engineered Rhodospirillum rubrum.
Heinrich D, Raberg M, Steinbüchel A., FEMS Microbiol Lett 362(8), 2015
PMID: 25761750
Heinrich D, Raberg M, Steinbüchel A., FEMS Microbiol Lett 362(8), 2015
PMID: 25761750
Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration.
Ng CY, Farasat I, Maranas CD, Salis HM., Metab Eng 29(), 2015
PMID: 25769287
Ng CY, Farasat I, Maranas CD, Salis HM., Metab Eng 29(), 2015
PMID: 25769287
A giant market and a powerful metabolism: L-lysine provided by Corynebacterium glutamicum.
Eggeling L, Bott M., Appl Microbiol Biotechnol 99(8), 2015
PMID: 25761623
Eggeling L, Bott M., Appl Microbiol Biotechnol 99(8), 2015
PMID: 25761623
Metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by Corynebacterium glutamicum.
Siebert D, Wendisch VF., Biotechnol Biofuels 8(), 2015
PMID: 26110019
Siebert D, Wendisch VF., Biotechnol Biofuels 8(), 2015
PMID: 26110019
Review and Hypothesis. New insights into the reaction mechanism of transhydrogenase: Swivelling the dIII component may gate the proton channel.
Jackson JB, Leung JH, Stout CD, Schurig-Briccio LA, Gennis RB., FEBS Lett 589(16), 2015
PMID: 26143375
Jackson JB, Leung JH, Stout CD, Schurig-Briccio LA, Gennis RB., FEBS Lett 589(16), 2015
PMID: 26143375
NADPH-generating systems in bacteria and archaea.
Spaans SK, Weusthuis RA, van der Oost J, Kengen SW., Front Microbiol 6(), 2015
PMID: 26284036
Spaans SK, Weusthuis RA, van der Oost J, Kengen SW., Front Microbiol 6(), 2015
PMID: 26284036
Anaerobic growth of Corynebacterium glutamicum via mixed-acid fermentation.
Michel A, Koch-Koerfges A, Krumbach K, Brocker M, Bott M., Appl Environ Microbiol 81(21), 2015
PMID: 26276118
Michel A, Koch-Koerfges A, Krumbach K, Brocker M, Bott M., Appl Environ Microbiol 81(21), 2015
PMID: 26276118
Heterologous expression of Escherichia coli fructose-1,6-bisphosphatase in Corynebacterium glutamicum and evaluating the effect on cell growth and L-lysine production.
Xu JZ, Zhang JL, Guo YF, Jia QD, Zhang WG., Prep Biochem Biotechnol 44(5), 2014
PMID: 24397720
Xu JZ, Zhang JL, Guo YF, Jia QD, Zhang WG., Prep Biochem Biotechnol 44(5), 2014
PMID: 24397720
Enhancement of L-ornithine production by disruption of three genes encoding putative oxidoreductases in Corynebacterium glutamicum.
Hwang GH, Cho JY., J Ind Microbiol Biotechnol 41(3), 2014
PMID: 24402505
Hwang GH, Cho JY., J Ind Microbiol Biotechnol 41(3), 2014
PMID: 24402505
Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering.
Cui YY, Ling C, Zhang YY, Huang J, Liu JZ., Microb Cell Fact 13(), 2014
PMID: 24512078
Cui YY, Ling C, Zhang YY, Huang J, Liu JZ., Microb Cell Fact 13(), 2014
PMID: 24512078
Development of Halomonas TD01 as a host for open production of chemicals.
Fu XZ, Tan D, Aibaidula G, Wu Q, Chen JC, Chen GQ., Metab Eng 23(), 2014
PMID: 24566041
Fu XZ, Tan D, Aibaidula G, Wu Q, Chen JC, Chen GQ., Metab Eng 23(), 2014
PMID: 24566041
Carbon flux analysis by 13C nuclear magnetic resonance to determine the effect of CO2 on anaerobic succinate production by Corynebacterium glutamicum.
Radoš D, Turner DL, Fonseca LL, Carvalho AL, Blombach B, Eikmanns BJ, Neves AR, Santos H., Appl Environ Microbiol 80(10), 2014
PMID: 24610842
Radoš D, Turner DL, Fonseca LL, Carvalho AL, Blombach B, Eikmanns BJ, Neves AR, Santos H., Appl Environ Microbiol 80(10), 2014
PMID: 24610842
Genome-scale metabolic network reconstruction of Saccharopolyspora spinosa for spinosad production improvement.
Wang X, Zhang C, Wang M, Lu W., Microb Cell Fact 13(1), 2014
PMID: 24628959
Wang X, Zhang C, Wang M, Lu W., Microb Cell Fact 13(1), 2014
PMID: 24628959
Application of metabolic engineering for the biotechnological production of L-valine.
Oldiges M, Eikmanns BJ, Blombach B., Appl Microbiol Biotechnol 98(13), 2014
PMID: 24816722
Oldiges M, Eikmanns BJ, Blombach B., Appl Microbiol Biotechnol 98(13), 2014
PMID: 24816722
Metabolic engineering Corynebacterium glutamicum for the L-lysine production by increasing the flux into L-lysine biosynthetic pathway.
Xu J, Han M, Zhang J, Guo Y, Zhang W., Amino Acids 46(9), 2014
PMID: 24879631
Xu J, Han M, Zhang J, Guo Y, Zhang W., Amino Acids 46(9), 2014
PMID: 24879631
Construction of a novel expression system for use in Corynebacterium glutamicum.
Hu J, Li Y, Zhang H, Tan Y, Wang X., Plasmid 75(), 2014
PMID: 25108235
Hu J, Li Y, Zhang H, Tan Y, Wang X., Plasmid 75(), 2014
PMID: 25108235
Effects of NADH kinase on NADPH-dependent biotransformation processes in Escherichia coli.
Lee WH, Kim JW, Park EH, Han NS, Kim MD, Seo JH., Appl Microbiol Biotechnol 97(4), 2013
PMID: 23053084
Lee WH, Kim JW, Park EH, Han NS, Kim MD, Seo JH., Appl Microbiol Biotechnol 97(4), 2013
PMID: 23053084
Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation.
Lee WH, Kim MD, Jin YS, Seo JH., Appl Microbiol Biotechnol 97(7), 2013
PMID: 23420268
Lee WH, Kim MD, Jin YS, Seo JH., Appl Microbiol Biotechnol 97(7), 2013
PMID: 23420268
Metabolic evolution of Corynebacterium glutamicum for increased production of L-ornithine.
Jiang LY, Chen SG, Zhang YY, Liu JZ., BMC Biotechnol 13(), 2013
PMID: 23725060
Jiang LY, Chen SG, Zhang YY, Liu JZ., BMC Biotechnol 13(), 2013
PMID: 23725060
Metabolic engineering and transhydrogenase effects on NADPH availability in Escherichia coli.
Jan J, Martinez I, Wang Y, Bennett GN, San KY., Biotechnol Prog 29(5), 2013
PMID: 23794523
Jan J, Martinez I, Wang Y, Bennett GN, San KY., Biotechnol Prog 29(5), 2013
PMID: 23794523
Metabolic engineering of Corynebacterium glutamicum for increasing the production of L-ornithine by increasing NADPH availability.
Jiang LY, Zhang YY, Li Z, Liu JZ., J Ind Microbiol Biotechnol 40(10), 2013
PMID: 23836141
Jiang LY, Zhang YY, Li Z, Liu JZ., J Ind Microbiol Biotechnol 40(10), 2013
PMID: 23836141
Recent advances in recombinant protein expression by Corynebacterium, Brevibacterium, and Streptomyces: from transcription and translation regulation to secretion pathway selection.
Liu L, Yang H, Shin HD, Li J, Du G, Chen J., Appl Microbiol Biotechnol 97(22), 2013
PMID: 24068337
Liu L, Yang H, Shin HD, Li J, Du G, Chen J., Appl Microbiol Biotechnol 97(22), 2013
PMID: 24068337
Improvement of the redox balance increases L-valine production by Corynebacterium glutamicum under oxygen deprivation conditions.
Hasegawa S, Uematsu K, Natsuma Y, Suda M, Hiraga K, Jojima T, Inui M, Yukawa H., Appl Environ Microbiol 78(3), 2012
PMID: 22138982
Hasegawa S, Uematsu K, Natsuma Y, Suda M, Hiraga K, Jojima T, Inui M, Yukawa H., Appl Environ Microbiol 78(3), 2012
PMID: 22138982
Corynebacterium glutamicum harbours a molybdenum cofactor-dependent formate dehydrogenase which alleviates growth inhibition in the presence of formate.
Witthoff S, Eggeling L, Bott M, Polen T., Microbiology 158(pt 9), 2012
PMID: 22767548
Witthoff S, Eggeling L, Bott M, Polen T., Microbiology 158(pt 9), 2012
PMID: 22767548
Optimization of enzyme parameters for fermentative production of biorenewable fuels and chemicals.
Jarboe LR, Liu P, Kautharapu KB, Ingram LO., Comput Struct Biotechnol J 3(), 2012
PMID: 24688665
Jarboe LR, Liu P, Kautharapu KB, Ingram LO., Comput Struct Biotechnol J 3(), 2012
PMID: 24688665
Cytosolic NADPH balancing in Penicillium chrysogenum cultivated on mixtures of glucose and ethanol.
Zhao Z, Kuijvenhoven K, van Gulik WM, Heijnen JJ, van Winden WA, Verheijen PJ., Appl Microbiol Biotechnol 89(1), 2011
PMID: 20809073
Zhao Z, Kuijvenhoven K, van Gulik WM, Heijnen JJ, van Winden WA, Verheijen PJ., Appl Microbiol Biotechnol 89(1), 2011
PMID: 20809073
Corynebacterium glutamicum tailored for efficient isobutanol production.
Blombach B, Riester T, Wieschalka S, Ziert C, Youn JW, Wendisch VF, Eikmanns BJ., Appl Environ Microbiol 77(10), 2011
PMID: 21441331
Blombach B, Riester T, Wieschalka S, Ziert C, Youn JW, Wendisch VF, Eikmanns BJ., Appl Environ Microbiol 77(10), 2011
PMID: 21441331
Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains.
Ruiz JC, D'Afonseca V, Silva A, Ali A, Pinto AC, Santos AR, Rocha AA, Lopes DO, Dorella FA, Pacheco LG, Costa MP, Turk MZ, Seyffert N, Moraes PM, Soares SC, Almeida SS, Castro TL, Abreu VA, Trost E, Baumbach J, Tauch A, Schneider MP, McCulloch J, Cerdeira LT, Ramos RT, Zerlotini A, Dominitini A, Resende DM, Coser EM, Oliveira LM, Pedrosa AL, Vieira CU, Guimarães CT, Bartholomeu DC, Oliveira DM, Santos FR, Rabelo ÉM, Lobo FP, Franco GR, Costa AF, Castro IM, Dias SR, Ferro JA, Ortega JM, Paiva LV, Goulart LR, Almeida JF, Ferro MI, Carneiro NP, Falcão PR, Grynberg P, Teixeira SM, Brommonschenkel S, Oliveira SC, Meyer R, Moore RJ, Miyoshi A, Oliveira GC, Azevedo V., PLoS One 6(4), 2011
PMID: 21533164
Ruiz JC, D'Afonseca V, Silva A, Ali A, Pinto AC, Santos AR, Rocha AA, Lopes DO, Dorella FA, Pacheco LG, Costa MP, Turk MZ, Seyffert N, Moraes PM, Soares SC, Almeida SS, Castro TL, Abreu VA, Trost E, Baumbach J, Tauch A, Schneider MP, McCulloch J, Cerdeira LT, Ramos RT, Zerlotini A, Dominitini A, Resende DM, Coser EM, Oliveira LM, Pedrosa AL, Vieira CU, Guimarães CT, Bartholomeu DC, Oliveira DM, Santos FR, Rabelo ÉM, Lobo FP, Franco GR, Costa AF, Castro IM, Dias SR, Ferro JA, Ortega JM, Paiva LV, Goulart LR, Almeida JF, Ferro MI, Carneiro NP, Falcão PR, Grynberg P, Teixeira SM, Brommonschenkel S, Oliveira SC, Meyer R, Moore RJ, Miyoshi A, Oliveira GC, Azevedo V., PLoS One 6(4), 2011
PMID: 21533164
Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli.
Bastian S, Liu X, Meyerowitz JT, Snow CD, Chen MM, Arnold FH., Metab Eng 13(3), 2011
PMID: 21515217
Bastian S, Liu X, Meyerowitz JT, Snow CD, Chen MM, Arnold FH., Metab Eng 13(3), 2011
PMID: 21515217
Enhanced production of GDP-L-fucose by overexpression of NADPH regenerator in recombinant Escherichia coli.
Lee WH, Chin YW, Han NS, Kim MD, Seo JH., Appl Microbiol Biotechnol 91(4), 2011
PMID: 21538115
Lee WH, Chin YW, Han NS, Kim MD, Seo JH., Appl Microbiol Biotechnol 91(4), 2011
PMID: 21538115
Comparative 13C metabolic flux analysis of pyruvate dehydrogenase complex-deficient, L-valine-producing Corynebacterium glutamicum.
Bartek T, Blombach B, Lang S, Eikmanns BJ, Wiechert W, Oldiges M, Nöh K, Noack S., Appl Environ Microbiol 77(18), 2011
PMID: 21784914
Bartek T, Blombach B, Lang S, Eikmanns BJ, Wiechert W, Oldiges M, Nöh K, Noack S., Appl Environ Microbiol 77(18), 2011
PMID: 21784914
Polyphosphate/ATP-dependent NAD kinase of Corynebacterium glutamicum: biochemical properties and impact of ppnK overexpression on lysine production.
Lindner SN, Niederholtmeyer H, Schmitz K, Schoberth SM, Wendisch VF., Appl Microbiol Biotechnol 87(2), 2010
PMID: 20180116
Lindner SN, Niederholtmeyer H, Schmitz K, Schoberth SM, Wendisch VF., Appl Microbiol Biotechnol 87(2), 2010
PMID: 20180116
Importance of NADPH supply for improved L-valine formation in Corynebacterium glutamicum.
Bartek T, Blombach B, Zönnchen E, Makus P, Lang S, Eikmanns BJ, Oldiges M., Biotechnol Prog 26(2), 2010
PMID: 20014412
Bartek T, Blombach B, Zönnchen E, Makus P, Lang S, Eikmanns BJ, Oldiges M., Biotechnol Prog 26(2), 2010
PMID: 20014412
Metabolic flux distributions: genetic information, computational predictions, and experimental validation.
Blank LM, Kuepfer L., Appl Microbiol Biotechnol 86(5), 2010
PMID: 20232063
Blank LM, Kuepfer L., Appl Microbiol Biotechnol 86(5), 2010
PMID: 20232063
Carbohydrate metabolism in Corynebacterium glutamicum and applications for the metabolic engineering of L-lysine production strains.
Blombach B, Seibold GM., Appl Microbiol Biotechnol 86(5), 2010
PMID: 20333512
Blombach B, Seibold GM., Appl Microbiol Biotechnol 86(5), 2010
PMID: 20333512
Engineering Corynebacterium glutamicum for isobutanol production.
Smith KM, Cho KM, Liao JC., Appl Microbiol Biotechnol 87(3), 2010
PMID: 20376637
Smith KM, Cho KM, Liao JC., Appl Microbiol Biotechnol 87(3), 2010
PMID: 20376637
Metabolic pathways and fermentative production of L-aspartate family amino acids.
Park JH, Lee SY., Biotechnol J 5(6), 2010
PMID: 20518059
Park JH, Lee SY., Biotechnol J 5(6), 2010
PMID: 20518059
Redox biocatalysis and metabolism: molecular mechanisms and metabolic network analysis.
Blank LM, Ebert BE, Buehler K, Bühler B., Antioxid Redox Signal 13(3), 2010
PMID: 20059399
Blank LM, Ebert BE, Buehler K, Bühler B., Antioxid Redox Signal 13(3), 2010
PMID: 20059399
Engineering of Corynebacterium glutamicum with an NADPH-generating glycolytic pathway for L-lysine production.
Takeno S, Murata R, Kobayashi R, Mitsuhashi S, Ikeda M., Appl Environ Microbiol 76(21), 2010
PMID: 20851994
Takeno S, Murata R, Kobayashi R, Mitsuhashi S, Ikeda M., Appl Environ Microbiol 76(21), 2010
PMID: 20851994
Acetohydroxyacid synthase, a novel target for improvement of L-lysine production by Corynebacterium glutamicum.
Blombach B, Hans S, Bathe B, Eikmanns BJ., Appl Environ Microbiol 75(2), 2009
PMID: 19047397
Blombach B, Hans S, Bathe B, Eikmanns BJ., Appl Environ Microbiol 75(2), 2009
PMID: 19047397
Co-ordinated regulation of gluconate catabolism and glucose uptake in Corynebacterium glutamicum by two functionally equivalent transcriptional regulators, GntR1 and GntR2.
Frunzke J, Engels V, Hasenbein S, Gätgens C, Bott M., Mol Microbiol 67(2), 2008
PMID: 18047570
Frunzke J, Engels V, Hasenbein S, Gätgens C, Bott M., Mol Microbiol 67(2), 2008
PMID: 18047570
Corynebacterium glutamicum tailored for high-yield L-valine production.
Blombach B, Schreiner ME, Bartek T, Oldiges M, Eikmanns BJ., Appl Microbiol Biotechnol 79(3), 2008
PMID: 18379776
Blombach B, Schreiner ME, Bartek T, Oldiges M, Eikmanns BJ., Appl Microbiol Biotechnol 79(3), 2008
PMID: 18379776
NCgl2620 encodes a class II polyphosphate kinase in Corynebacterium glutamicum.
Lindner SN, Vidaurre D, Willbold S, Schoberth SM, Wendisch VF., Appl Environ Microbiol 73(15), 2007
PMID: 17545325
Lindner SN, Vidaurre D, Willbold S, Schoberth SM, Wendisch VF., Appl Environ Microbiol 73(15), 2007
PMID: 17545325
42 References
Daten bereitgestellt von Europe PubMed Central.
S, J Gen Appl Microbiol 13(), 1967
Expression of the Escherichia coli pntA and pntB genes, encoding nicotinamide nucleotide transhydrogenase, in Saccharomyces cerevisiae and its effect on product formation during anaerobic glucose fermentation.
Anderlund M, Nissen TL, Nielsen J, Villadsen J, Rydstrom J, Hahn-Hagerdal B, Kielland-Brandt MC., Appl. Environ. Microbiol. 65(6), 1999
PMID: 10347010
Anderlund M, Nissen TL, Nielsen J, Villadsen J, Rydstrom J, Hahn-Hagerdal B, Kielland-Brandt MC., Appl. Environ. Microbiol. 65(6), 1999
PMID: 10347010
F, 1992
The respiratory chain of Corynebacterium glutamicum.
Bott M, Niebisch A., J. Biotechnol. 104(1-3), 2003
PMID: 12948635
Bott M, Niebisch A., J. Biotechnol. 104(1-3), 2003
PMID: 12948635
M, 2005
Cloning and expression of the transhydrogenase gene of Escherichia coli.
Clarke DM, Bragg PD., J. Bacteriol. 162(1), 1985
PMID: 3884596
Clarke DM, Bragg PD., J. Bacteriol. 162(1), 1985
PMID: 3884596
Complete Sucrose Metabolism Requires Fructose Phosphotransferase Activity in Corynebacterium glutamicum To Ensure Phosphorylation of Liberated Fructose.
Dominguez H, Lindley ND., Appl. Environ. Microbiol. 62(10), 1996
PMID: 16535429
Dominguez H, Lindley ND., Appl. Environ. Microbiol. 62(10), 1996
PMID: 16535429
Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose.
Dominguez H, Rollin C, Guyonvarch A, Guerquin-Kern JL, Cocaign-Bousquet M, Lindley ND., Eur. J. Biochem. 254(1), 1998
PMID: 9652400
Dominguez H, Rollin C, Guyonvarch A, Guerquin-Kern JL, Cocaign-Bousquet M, Lindley ND., Eur. J. Biochem. 254(1), 1998
PMID: 9652400
Biology of L-lysine overproduction byCorynebacterium glutamicum.
Eggeling L., Amino Acids 6(3), 1994
PMID: 24189734
Eggeling L., Amino Acids 6(3), 1994
PMID: 24189734
L, Appl Microbiol Biotechnol 52(), 1999
A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing.
Eikmanns BJ, Kleinertz E, Liebl W, Sahm H., Gene 102(1), 1991
PMID: 1864513
Eikmanns BJ, Kleinertz E, Liebl W, Sahm H., Gene 102(1), 1991
PMID: 1864513
Cloning, sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme.
Eikmanns BJ, Rittmann D, Sahm H., J. Bacteriol. 177(3), 1995
PMID: 7836312
Eikmanns BJ, Rittmann D, Sahm H., J. Bacteriol. 177(3), 1995
PMID: 7836312
Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: roles of malic enzyme and fructose-1,6-bisphosphatase.
Georgi T, Rittmann D, Wendisch VF., Metab. Eng. 7(4), 2005
PMID: 15979917
Georgi T, Rittmann D, Wendisch VF., Metab. Eng. 7(4), 2005
PMID: 15979917
Cloning of the malic enzyme gene from Corynebacterium glutamicum and role of the enzyme in lactate metabolism.
Gourdon P, Baucher MF, Lindley ND, Guyonvarch A., Appl. Environ. Microbiol. 66(7), 2000
PMID: 10877795
Gourdon P, Baucher MF, Lindley ND, Guyonvarch A., Appl. Environ. Microbiol. 66(7), 2000
PMID: 10877795
D, 1985
Industrial production of amino acids by coryneform bacteria.
Hermann T., J. Biotechnol. 104(1-3), 2003
PMID: 12948636
Hermann T., J. Biotechnol. 104(1-3), 2003
PMID: 12948636
R, 2005
C, J Bacteriol 17(), 1993
Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose.
Kiefer P, Heinzle E, Zelder O, Wittmann C., Appl. Environ. Microbiol. 70(1), 2004
PMID: 14711646
Kiefer P, Heinzle E, Zelder O, Wittmann C., Appl. Environ. Microbiol. 70(1), 2004
PMID: 14711646
E, 2005
The ptsI gene encoding enzyme I of the phosphotransferase system of Corynebacterium glutamicum.
Kotrba P, Inui M, Yukawa H., Biochem. Biophys. Res. Commun. 289(5), 2001
PMID: 11741338
Kotrba P, Inui M, Yukawa H., Biochem. Biophys. Res. Commun. 289(5), 2001
PMID: 11741338
W, 2005
P, Anal Chem 51(), 1979
Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing.
Marx A, de Graaf AA, Wiechert W, Eggeling L, Sahm H., Biotechnol. Bioeng. 49(2), 1996
PMID: 18623562
Marx A, de Graaf AA, Wiechert W, Eggeling L, Sahm H., Biotechnol. Bioeng. 49(2), 1996
PMID: 18623562
Response of the central metabolism of Corynebacterium glutamicum to different flux burdens.
Marx A, Striegel K, de Graaf AA, Sahm H, Eggeling L., Biotechnol. Bioeng. 56(2), 1997
PMID: 18636622
Marx A, Striegel K, de Graaf AA, Sahm H, Eggeling L., Biotechnol. Bioeng. 56(2), 1997
PMID: 18636622
Response of the central metabolism in Corynebacterium glutamicum to the use of an NADH-dependent glutamate dehydrogenase.
Marx A, Eikmanns BJ, Sahm H, de Graaf AA, Eggeling L., Metab. Eng. 1(1), 1999
PMID: 10935753
Marx A, Eikmanns BJ, Sahm H, de Graaf AA, Eggeling L., Metab. Eng. 1(1), 1999
PMID: 10935753
Metabolic phenotype of phosphoglucose isomerase mutants of Corynebacterium glutamicum.
Marx A, Hans S, Mockel B, Bathe B, de Graaf AA, McCormack AC, Stapleton C, Burke K, O'Donohue M, Dunican LK., J. Biotechnol. 104(1-3), 2003
PMID: 12948638
Marx A, Hans S, Mockel B, Bathe B, de Graaf AA, McCormack AC, Stapleton C, Burke K, O'Donohue M, Dunican LK., J. Biotechnol. 104(1-3), 2003
PMID: 12948638
Analyses of enzyme II gene mutants for sugar transport and heterologous expression of fructokinase gene in Corynebacterium glutamicum ATCC 13032.
Moon MW, Kim HJ, Oh TK, Shin CS, Lee JS, Kim SJ, Lee JK., FEMS Microbiol. Lett. 244(2), 2005
PMID: 15766777
Moon MW, Kim HJ, Oh TK, Shin CS, Lee JS, Kim SJ, Lee JK., FEMS Microbiol. Lett. 244(2), 2005
PMID: 15766777
Kinetic properties of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Corynebacterium glutamicum and their application for predicting pentose phosphate pathway flux in vivo.
Moritz B, Striegel K, De Graaf AA, Sahm H., Eur. J. Biochem. 267(12), 2000
PMID: 10848959
Moritz B, Striegel K, De Graaf AA, Sahm H., Eur. J. Biochem. 267(12), 2000
PMID: 10848959
A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine-producing mutant.
Ohnishi J, Mitsuhashi S, Hayashi M, Ando S, Yokoi H, Ochiai K, Ikeda M., Appl. Microbiol. Biotechnol. 58(2), 2002
PMID: 11876415
Ohnishi J, Mitsuhashi S, Hayashi M, Ando S, Yokoi H, Ochiai K, Ikeda M., Appl. Microbiol. Biotechnol. 58(2), 2002
PMID: 11876415
Corynebacterium glutamicum: a dissection of the PTS.
Parche S, Burkovski A, Sprenger GA, Weil B, Kramer R, Titgemeyer F., J. Mol. Microbiol. Biotechnol. 3(3), 2001
PMID: 11361073
Parche S, Burkovski A, Sprenger GA, Weil B, Kramer R, Titgemeyer F., J. Mol. Microbiol. Biotechnol. 3(3), 2001
PMID: 11361073
Biotechnological manufacture of lysine.
Pfefferle W, Mockel B, Bathe B, Marx A., Adv. Biochem. Eng. Biotechnol. 79(), 2003
PMID: 12523389
Pfefferle W, Mockel B, Bathe B, Marx A., Adv. Biochem. Eng. Biotechnol. 79(), 2003
PMID: 12523389
A, Biotechnol Bioeng 51(), 1996
Ethambutol, a cell wall inhibitor of Mycobacterium tuberculosis, elicits L-glutamate efflux of Corynebacterium glutamicum.
Radmacher E, Stansen KC, Besra GS, Alderwick LJ, Maughan WN, Hollweg G, Sahm H, Wendisch VF, Eggeling L., Microbiology (Reading, Engl.) 151(Pt 5), 2005
PMID: 15870446
Radmacher E, Stansen KC, Besra GS, Alderwick LJ, Maughan WN, Hollweg G, Sahm H, Wendisch VF, Eggeling L., Microbiology (Reading, Engl.) 151(Pt 5), 2005
PMID: 15870446
Fructose-1,6-bisphosphatase from Corynebacterium glutamicum: expression and deletion of the fbp gene and biochemical characterization of the enzyme.
Rittmann D, Schaffer S, Wendisch VF, Sahm H., Arch. Microbiol. 180(4), 2003
PMID: 12904832
Rittmann D, Schaffer S, Wendisch VF, Sahm H., Arch. Microbiol. 180(4), 2003
PMID: 12904832
Pathway analysis and metabolic engineering in Corynebacterium glutamicum.
Sahm H, Eggeling L, de Graaf AA., Biol. Chem. 381(9-10), 2000
PMID: 11076021
Sahm H, Eggeling L, de Graaf AA., Biol. Chem. 381(9-10), 2000
PMID: 11076021
The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli.
Sauer U, Canonaco F, Heri S, Perrenoud A, Fischer E., J. Biol. Chem. 279(8), 2003
PMID: 14660605
Sauer U, Canonaco F, Heri S, Perrenoud A, Fischer E., J. Biol. Chem. 279(8), 2003
PMID: 14660605
Measurement of protein using bicinchoninic acid.
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC., Anal. Biochem. 150(1), 1985
PMID: 3843705
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC., Anal. Biochem. 150(1), 1985
PMID: 3843705
A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA.
van der Rest ME, Lange C, Molenaar D., Appl. Microbiol. Biotechnol. 52(4), 1999
PMID: 10570802
van der Rest ME, Lange C, Molenaar D., Appl. Microbiol. Biotechnol. 52(4), 1999
PMID: 10570802
JD, Biochem J 341(), 1999
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