Acetate metabolism and its regulation in Corynebacterium glutamicum

Gerstmeir R, Wendisch VF, Schnicke S, Ruan H, Farwick M, Reinscheid D, Eikmanns BJ (2003)
Journal of Biotechnology 104(1-3): 99-122.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ; ;
Abstract / Bemerkung
The amino acid producing Corynebacterium glutamicum grows aerobically on a variety of carbohydrates and organic acids as single or combined sources of carbon and energy. Among the substrates metabolized are glucose and acetate which both can also serve as substrates for amino acid production. Based on biochemical, genetic and regulatory studies and on quantitative determination of metabolic fluxes during utilization of acetate and/or glucose, this review summarizes the present knowledge on the different steps of the fundamental pathways of acetate utilization in C glutamicum, namely, on acetate transport, acetate activation, tricarboxylic acid (TCA) cycle, glyoxylate cycle and gluconeogenesis. It becomes evident that, although the pathways of acetate utilization follow the same theme in many bacteria, important biochemical, genetic and regulatory peculiarities exist in C glutamicum. Recent genome wide and comparative expression analyses in C glutamicum cells grown on glucose and on acetate substantiated previously identified transcriptional regulation of acetate activating enzymes and of glyoxylate cycle enzymes. Additionally, a variety of genes obviously also under transcriptional control in response to the presence or absence of acetate in the growth medium were uncovered. These genes, thus also belonging to the acetate stimulon of C glutamicum, include genes coding for TCA cycle enzymes (e.g. aconitase and succinate dehydrogenase), for gluconeogenesis (phosphoenolpyruvate carboxykinase), for glycolysis (pyruvate dehydrogenase El) and genes coding for proteins with hitherto unknown function. Although the basic mechanism of transcriptional regulation of the enzymes involved in acetate metabolism is not yet understood, some recent findings led to a better understanding of the adaptation of C glutamicum to acetate at the molecular level. (C) 2003 Elsevier B.V. All rights reserved.
Stichworte
tricarboxylic-acid cycle; escherichia-coli k12; gram-positive bacteria; acetate metabolism; corynebacterium glutamicum; acetate activation; glyoxylate cycle; carbon flux regulation; expression profiling; phosphoenolpyruvate carboxykinase gene; cytoplasmic malate-dehydrogenases; sequence-analysis; pyruvate-carboxylase; bacillus-subtilis acetate; carbon catabolite repression; isocitrate dehydrogenase
Erscheinungsjahr
2003
Zeitschriftentitel
Journal of Biotechnology
Band
104
Ausgabe
1-3
Seite(n)
99-122
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1895000

Zitieren

Gerstmeir R, Wendisch VF, Schnicke S, et al. Acetate metabolism and its regulation in Corynebacterium glutamicum. Journal of Biotechnology. 2003;104(1-3):99-122.
Gerstmeir, R., Wendisch, V. F., Schnicke, S., Ruan, H., Farwick, M., Reinscheid, D., & Eikmanns, B. J. (2003). Acetate metabolism and its regulation in Corynebacterium glutamicum. Journal of Biotechnology, 104(1-3), 99-122. doi:10.1016/S0168-1656(03)00167-6
Gerstmeir, R., Wendisch, V. F., Schnicke, S., Ruan, H., Farwick, M., Reinscheid, D., and Eikmanns, B. J. (2003). Acetate metabolism and its regulation in Corynebacterium glutamicum. Journal of Biotechnology 104, 99-122.
Gerstmeir, R., et al., 2003. Acetate metabolism and its regulation in Corynebacterium glutamicum. Journal of Biotechnology, 104(1-3), p 99-122.
R. Gerstmeir, et al., “Acetate metabolism and its regulation in Corynebacterium glutamicum”, Journal of Biotechnology, vol. 104, 2003, pp. 99-122.
Gerstmeir, R., Wendisch, V.F., Schnicke, S., Ruan, H., Farwick, M., Reinscheid, D., Eikmanns, B.J.: Acetate metabolism and its regulation in Corynebacterium glutamicum. Journal of Biotechnology. 104, 99-122 (2003).
Gerstmeir, R., Wendisch, Volker F., Schnicke, S., Ruan, H., Farwick, M., Reinscheid, D., and Eikmanns, B. J. “Acetate metabolism and its regulation in Corynebacterium glutamicum”. Journal of Biotechnology 104.1-3 (2003): 99-122.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 12948633
PubMed | Europe PMC

Suchen in

Google Scholar