Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase

Georgi T, Rittmann D, Wendisch VF (2005)
Metabolic Engineering 7(4): 291-301.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Georgi, T.; Rittmann, D.; Wendisch, Volker F.UniBi
Abstract / Bemerkung
In the biotechnological production Of L-lysine and L-glutamate by Corynebacterium glutamicum media based on glucose, fructose or sucrose are typically used. Glutamate production by C glutamicum ATCC13032 was very similar on glucose, fructose, glucose plus fructose and sucrose. In contrast, lysine production of genetically defined C glutamicum strains was significantly higher on glucose than on the other carbon sources. To test whether malic enzyme or fructose-1,6-bisphosphatase might limit growth and lysine on fructose, glucose plus fructose or sucrose, strains overexpressing either malE which encodes the NADPH-dependent malic enzyme or the fructose-1,6-bisphosphatase gene fbp were generated. Overexpression of malE did not improve lysine production on any of the tested carbon sources. Upon overexpression of fbp lysine yields on glucose and/or fructose were unchanged, but the lysine yield on sucrose increased twofold. Thus, fructose-1,6-bisphosphatase was identified as a limiting factor for lysine production by C glutamicum with sucrose as the carbon source. (c) 2005 Elsevier Inc. All rights reserved.
flux; gene; amino-acids; biochemical-characterization; carbon-sources; in-vivo; pyruvate-carboxylase; brevibacterium-flavum; fbp; 6-bisphosphatase; fructose-1; male; malic enzyme; glucose; sucrose; glutamate production; fructose; corynebacterium glutamicum; lysine production; acetate metabolism; escherichia-coli
Metabolic Engineering
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Georgi T, Rittmann D, Wendisch VF. Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering. 2005;7(4):291-301.
Georgi, T., Rittmann, D., & Wendisch, V. F. (2005). Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering, 7(4), 291-301.
Georgi, T., Rittmann, D., and Wendisch, Volker F. 2005. “Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase”. Metabolic Engineering 7 (4): 291-301.
Georgi, T., Rittmann, D., and Wendisch, V. F. (2005). Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering 7, 291-301.
Georgi, T., Rittmann, D., & Wendisch, V.F., 2005. Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering, 7(4), p 291-301.
T. Georgi, D. Rittmann, and V.F. Wendisch, “Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase”, Metabolic Engineering, vol. 7, 2005, pp. 291-301.
Georgi, T., Rittmann, D., Wendisch, V.F.: Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metabolic Engineering. 7, 291-301 (2005).
Georgi, T., Rittmann, D., and Wendisch, Volker F. “Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase”. Metabolic Engineering 7.4 (2005): 291-301.

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