Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli

Blumer C, Kleefeld A, Lehnen D, Heintz M, Dobrindt U, Nagy G, Michaelis K, Emody L, Polen T, Rachel R, Wendisch VF, et al. (2005)
Microbiology-Sgm 151(10): 3287-3298.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Blumer, C.; Kleefeld, A.; Lehnen, D.; Heintz, M.; Dobrindt, U.; Nagy, G.; Michaelis, K.; Emody, L.; Polen, T.; Rachel, R.; Wendisch, Volker F.UniBi ; Unden, G.
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Abstract / Bemerkung
Type 1 fimbriae of Escherichia coli facilitate attachment to the host mucosa and promote biofilm formation on abiotic surfaces. The transcriptional regulator LrhA, which is known as a repressor of flagellar, motility and chennotaxis genes, regulates biofilm formation and expression of type 1 fimbriae. Whole-genome expression profiling revealed that inactivation of lrhA results in an increased expression of structural components of type 1 fimbriae. In vitro, LrhA bound to the promoter regions of the two fim recombinases (FimB and FimE) that catalyse the inversion of the fimA promoter, and to the invertible element itself. Translational lacZ fusions with these genes and quantification of fimE transcript levels by real-time PCR showed that LrhA influences type 1 fimbrial phase variation, primarily via activation of FimE, which is required for the ON-to-OFF transition of the fim switch. Enhanced type 1 fimbrial expression as a result of lrhA disruption was confirmed by mannose-sensitive agglutination of yeast cells. Biofilm formation was stimulated by lrhA inactivation and completely suppressed upon LrhA overproduction. The effects of LrhA on biofilm formation were exerted via the changed levels of surface molecules, most probably both flagella and type 1 fimbriae. Together, the data show a role for LrhA as a repressor of type 1 fimbrial expression, and thus as a regulator of the initial stages of biofilm development and, presumably, bacterial adherence to epithelial host cells also.
Stichworte
urinary-tract; suicide vector; genetic-analysis; global regulator; fim switch; h-ns; virulence determinants; phase variation; integration host factor; lysr homolog
Erscheinungsjahr
2005
Zeitschriftentitel
Microbiology-Sgm
Band
151
Ausgabe
10
Seite(n)
3287-3298
ISSN
1350-0872
eISSN
1465-2080
Page URI
https://pub.uni-bielefeld.de/record/1894950

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Blumer C, Kleefeld A, Lehnen D, et al. Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology-Sgm. 2005;151(10):3287-3298.
Blumer, C., Kleefeld, A., Lehnen, D., Heintz, M., Dobrindt, U., Nagy, G., Michaelis, K., et al. (2005). Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology-Sgm, 151(10), 3287-3298. doi:10.1099/mic.0.28098-0
Blumer, C., Kleefeld, A., Lehnen, D., Heintz, M., Dobrindt, U., Nagy, G., Michaelis, K., Emody, L., Polen, T., Rachel, R., et al. (2005). Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology-Sgm 151, 3287-3298.
Blumer, C., et al., 2005. Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology-Sgm, 151(10), p 3287-3298.
C. Blumer, et al., “Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli”, Microbiology-Sgm, vol. 151, 2005, pp. 3287-3298.
Blumer, C., Kleefeld, A., Lehnen, D., Heintz, M., Dobrindt, U., Nagy, G., Michaelis, K., Emody, L., Polen, T., Rachel, R., Wendisch, V.F., Unden, G.: Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology-Sgm. 151, 3287-3298 (2005).
Blumer, C., Kleefeld, A., Lehnen, D., Heintz, M., Dobrindt, U., Nagy, G., Michaelis, K., Emody, L., Polen, T., Rachel, R., Wendisch, Volker F., and Unden, G. “Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli”. Microbiology-Sgm 151.10 (2005): 3287-3298.

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