Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa
Hallmann A, Wodniok S (2006)
Plant Cell Rep. 25(6): 582-591.
Zeitschriftenaufsatz
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Autor*in
Hallmann, ArminUniBi
;
Wodniok, S.
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Abstract / Bemerkung
Production of transgenic organisms is a well-established, versatile course of action in molecular biology. Genetic engineering often requires heterologous, dominant antibiotic resistance genes that have been used as selectable markers in many species. However, as heterologous 5' and 3' flanking sequences often result in very low expression rates, endogenous flanking sequences, especially promoters, are mostly required and are easily obtained in model organisms, but it is much more complicated and time-consuming to get appropriate sequences from less common organisms. In this paper, we show that aminoglycoside 3'-phosphotransferase gene (aphVIII) based constructs with 3' and 5' untranslated flanking sequences (including promoters) from the multicellular green alga Volvox work in the unicellular green alga Chlamydomonas and flanking sequences from Chlamydomonas work in Volvox, at least if a low expression rate is compensated by an enforced high gene dosage. This strategy might be useful for all investigators that intend to transform species in which genomic sequences are not available, but sequences from related organisms exist.
Stichworte
paromomycin resistance;
transgenic;
volvocales;
green algae;
recombinant DNA technology;
genetic transformation;
expression
Erscheinungsjahr
2006
Zeitschriftentitel
Plant Cell Rep.
Band
25
Ausgabe
6
Seite(n)
582-591
ISSN
0721-7714
eISSN
1432-203X
Page URI
https://pub.uni-bielefeld.de/record/1870263
Zitieren
Hallmann A, Wodniok S. Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa. Plant Cell Rep. 2006;25(6):582-591.
Hallmann, A., & Wodniok, S. (2006). Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa. Plant Cell Rep., 25(6), 582-591. https://doi.org/10.1007/s00299-006-0121-x
Hallmann, Armin, and Wodniok, S. 2006. “Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa”. Plant Cell Rep. 25 (6): 582-591.
Hallmann, A., and Wodniok, S. (2006). Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa. Plant Cell Rep. 25, 582-591.
Hallmann, A., & Wodniok, S., 2006. Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa. Plant Cell Rep., 25(6), p 582-591.
A. Hallmann and S. Wodniok, “Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa”, Plant Cell Rep., vol. 25, 2006, pp. 582-591.
Hallmann, A., Wodniok, S.: Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa. Plant Cell Rep. 25, 582-591 (2006).
Hallmann, Armin, and Wodniok, S. “Swapped green algal promoters: aphVIII-based gene constructs with Chlamydomonas flanking sequences work as dominant selectable markers in Volvox and vice versa”. Plant Cell Rep. 25.6 (2006): 582-591.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
11 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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Hirata R, Takahashi M, Saga N, Mikami K., Mar Biotechnol (NY) 13(5), 2011
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Stable nuclear transformation of Gonium pectorale.
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Kianianmomeni A, Nematollahi G, Hallmann A., Plant Cell 20(9), 2008
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