Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system

Hartmann U, Valentine WJ, Christie JM, Hays J, Jenkins GI, Weisshaar B (1998)
Plant Molecular Biology 36(5): 741-754.

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Autor*in
Hartmann, U.; Valentine, W.J.; Christie, J.M.; Hays, J; Jenkins, G.I.; Weisshaar, BerndUniBi
Abstract / Bemerkung
To identify DNA sequences of the Arabidopsis thaliana chalcone synthase gene (CHS) concerned with induction by UV-B and UV-A/blue light, AtCHS promoter constructions were assayed by transient expression in protoplasts prepared from two different lines of cultured A. thaliana cells. The protoplasts responded similarly to A. thaliana leaf tissue in light-dependent CHS transcript accumulation. The reporter enzyme beta-glucuronidase (GUS) was used to monitor light-responsive promoter activity. A 1972 bp promoter conferred UV-B and UV-A/blue light induction of GUS activity. Deletion to 164 bp resulted in reduced promoter strength but retention of responsiveness to UV-B and UV-A/blue light. Further deletion abolished transcriptional activity. The 164 bp promoter contains sequences closely resembling LRUPcCHS, (light-responsive unit of the Petroselinum crispum CHS promoter). This A. thaliana CHS promoter region, designated LRUAtCHS, was sufficient to confer UV-B and UV-A/blue light responsiveness to a heterologous core promoter. Mutation of sequences in LRUAtCHS corresponding to the ACGT element and the MYB recognition element of LRUPcCHS resulted in inactivation of the 164 bp and 335 bp promoter deletions. However, the mutant 668 bp promoter retained residual UV-B and UV-A/blue light-induced expression, indicating the presence of additional functional sequences upstream of -335. Mutation of a single G-box-like sequence around -442 had no effect on light responsiveness, indicating that it does not function in light regulation of this promoter. Since no difference in responsiveness to UV-B and UV-A/blue light was observed with any promoter variant, we conclude that the two phototransduction pathways regulate transcription factors which interact with common promoter elements. The results from-our analysis of a A. thaliana light-responsive promoter will facilitate the study of light-dependent gene regulation by genetic means in Arabidopsis thaliana.
Erscheinungsjahr
1998
Zeitschriftentitel
Plant Molecular Biology
Band
36
Ausgabe
5
Seite(n)
741-754
ISSN
0167-4412
Page URI
https://pub.uni-bielefeld.de/record/1867951

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Hartmann U, Valentine WJ, Christie JM, Hays J, Jenkins GI, Weisshaar B. Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology. 1998;36(5):741-754.
Hartmann, U., Valentine, W. J., Christie, J. M., Hays, J., Jenkins, G. I., & Weisshaar, B. (1998). Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology, 36(5), 741-754. https://doi.org/10.1023/A:1005921914384
Hartmann, U., Valentine, W. J., Christie, J. M., Hays, J., Jenkins, G. I., and Weisshaar, B. (1998). Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology 36, 741-754.
Hartmann, U., et al., 1998. Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology, 36(5), p 741-754.
U. Hartmann, et al., “Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system”, Plant Molecular Biology, vol. 36, 1998, pp. 741-754.
Hartmann, U., Valentine, W.J., Christie, J.M., Hays, J., Jenkins, G.I., Weisshaar, B.: Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Molecular Biology. 36, 741-754 (1998).
Hartmann, U., Valentine, W.J., Christie, J.M., Hays, J, Jenkins, G.I., and Weisshaar, Bernd. “Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system”. Plant Molecular Biology 36.5 (1998): 741-754.

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Daten bereitgestellt von Europe PubMed Central.

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Zhang N, Sun Q, Li H, Li X, Cao Y, Zhang H, Li S, Zhang L, Qi Y, Ren S, Zhao B, Guo YD., Front Plant Sci 7(), 2016
PMID: 27047496
Molecular Characterization of MaCCS, a Novel Copper Chaperone Gene Involved in Abiotic and Hormonal Stress Responses in Musa acuminata cv. Tianbaojiao.
Feng X, Chen F, Liu W, Thu MK, Zhang Z, Chen Y, Cheng C, Lin Y, Wang T, Lai Z., Int J Mol Sci 17(4), 2016
PMID: 27023517
Chalcone synthase genes from milk thistle (Silybum marianum): isolation and expression analysis.
Sanjari S, Shobbar ZS, Ebrahimi M, Hasanloo T, Sadat-Noori SA, Tirnaz S., J Genet 94(4), 2015
PMID: 26690515
UVR8 mediated plant protective responses under low UV-B radiation leading to photosynthetic acclimation.
Singh S, Agrawal SB, Agrawal M., J Photochem Photobiol B 137(), 2014
PMID: 24780386
Footprints of the sun: memory of UV and light stress in plants.
Müller-Xing R, Xing Q, Goodrich J., Front Plant Sci 5(), 2014
PMID: 25278950
Genome-wide identification and characterisation of R2R3-MYB genes in sugar beet (Beta vulgaris).
Stracke R, Holtgräwe D, Schneider J, Pucker B, Sörensen TR, Weisshaar B., BMC Plant Biol 14(), 2014
PMID: 25249410
The Magnaporthe oryzae effector AVR1-CO39 is translocated into rice cells independently of a fungal-derived machinery.
Ribot C, Césari S, Abidi I, Chalvon V, Bournaud C, Vallet J, Lebrun MH, Morel JB, Kroj T., Plant J 74(1), 2013
PMID: 23279638
Lifetimes of Arabidopsis cryptochrome signaling states in vivo.
Herbel V, Orth C, Wenzel R, Ahmad M, Bittl R, Batschauer A., Plant J 74(4), 2013
PMID: 23398192
Dynamics of the shade-avoidance response in Arabidopsis.
Ciolfi A, Sessa G, Sassi M, Possenti M, Salvucci S, Carabelli M, Morelli G, Ruberti I., Plant Physiol 163(1), 2013
PMID: 23893169
Heterologous expression of gentian MYB1R transcription factors suppresses anthocyanin pigmentation in tobacco flowers.
Nakatsuka T, Yamada E, Saito M, Fujita K, Nishihara M., Plant Cell Rep 32(12), 2013
PMID: 24037114
A new system for fast and quantitative analysis of heterologous gene expression in plants.
Thévenin J, Dubos C, Xu W, Le Gourrierec J, Kelemen Z, Charlot F, Nogué F, Lepiniec L, Dubreucq B., New Phytol 193(2), 2012
PMID: 22023451
UV-B signaling pathways and fluence rate dependent transcriptional regulation of ARIADNE12.
Lang-Mladek C, Xie L, Nigam N, Chumak N, Binkert M, Neubert S, Hauser MT., Physiol Plant 145(4), 2012
PMID: 22188380
UV‐B signaling pathways and fluence rate dependent transcriptional regulation of ARIADNE12
Lang‐Mladek C, Xie L, Nigam N, Chumak N, Binkert M, Neubert S, Hauser M., Physiol Plant 145(4), 2012
PMID: IND44687575
The pea SAD short-chain dehydrogenase/reductase: quinone reduction, tissue distribution, and heterologous expression.
Scherbak N, Ala-Häivälä A, Brosché M, Böwer N, Strid H, Gittins JR, Grahn E, Eriksson LA, Strid Å., Plant Physiol 155(4), 2011
PMID: 21343423
Auxin and ethylene induce flavonol accumulation through distinct transcriptional networks.
Lewis DR, Ramirez MV, Miller ND, Vallabhaneni P, Ray WK, Helm RF, Winkel BS, Muday GK., Plant Physiol 156(1), 2011
PMID: 21427279
Chalcone synthase and its functions in plant resistance.
Dao TT, Linthorst HJ, Verpoorte R., Phytochem Rev 10(3), 2011
PMID: 21909286
The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1/MYB12 gene in response to light and ultraviolet-B radiation.
Stracke R, Favory JJ, Gruber H, Bartelniewoehner L, Bartels S, Binkert M, Funk M, Weisshaar B, Ulm R., Plant Cell Environ 33(1), 2010
PMID: 19895401
Luminescence detection of SNARE-SNARE interaction in Arabidopsis protoplasts.
Kato N, Fujikawa Y, Fuselier T, Adamou-Dodo R, Nishitani A, Sato MH., Plant Mol Biol 72(4-5), 2010
PMID: 20012673
Cloning and characterization of a UV-B-inducible maize flavonol synthase.
Falcone Ferreyra ML, Rius S, Emiliani J, Pourcel L, Feller A, Morohashi K, Casati P, Grotewold E., Plant J 62(1), 2010
PMID: 20059741
Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10.
Feng S, Wang Y, Yang S, Xu Y, Chen X., Planta 232(1), 2010
PMID: 20422209
Signal transduction in responses to UV-B radiation.
Jenkins GI., Annu Rev Plant Biol 60(), 2009
PMID: 19400728
Comprehensive prediction of novel microRNA targets in Arabidopsis thaliana.
Alves L, Niemeier S, Hauenschild A, Rehmsmeier M, Merkle T., Nucleic Acids Res 37(12), 2009
PMID: 19417064
An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis.
Mu RL, Cao YR, Liu YF, Lei G, Zou HF, Liao Y, Wang HW, Zhang WK, Ma B, Du JZ, Yuan M, Zhang JS, Chen SY., Cell Res 19(11), 2009
PMID: 19581938
Chemically induced and light-independent cryptochrome photoreceptor activation.
Rosenfeldt G, Viana RM, Mootz HD, von Arnim AG, Batschauer A., Mol Plant 1(1), 2008
PMID: 20031911
Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana.
Cominelli E, Gusmaroli G, Allegra D, Galbiati M, Wade HK, Jenkins GI, Tonelli C., J Plant Physiol 165(8), 2008
PMID: 17766004
Identification of a novel cis-regulatory element for UV-B-induced transcription in Arabidopsis.
Safrany J, Haasz V, Mate Z, Ciolfi A, Feher B, Oravecz A, Stec A, Dallmann G, Morelli G, Ulm R, Nagy F., Plant J 54(3), 2008
PMID: 18266923
Soybean GmMYB76, GmMYB92, and GmMYB177 genes confer stress tolerance in transgenic Arabidopsis plants.
Liao Y, Zou HF, Wang HW, Zhang WK, Ma B, Zhang JS, Chen SY., Cell Res 18(10), 2008
PMID: 18725908
A simplified method for the analysis of transcription factor-promoter interactions that allows high-throughput data generation.
Berger B, Stracke R, Yatusevich R, Weisshaar B, Flügge UI, Gigolashvili T., Plant J 50(5), 2007
PMID: 17425717
Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system.
Chiera JM, Bouchard RA, Dorsey SL, Park E, Buenrostro-Nava MT, Ling PP, Finer JJ., Plant Cell Rep 26(9), 2007
PMID: 17503049
Cis-regulatory evolution of chalcone-synthase expression in the genus Arabidopsis.
de Meaux J, Pop A, Mitchell-Olds T., Genetics 174(4), 2006
PMID: 17028316
The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis.
Mehrtens F, Kranz H, Bednarek P, Weisshaar B., Plant Physiol 138(2), 2005
PMID: 15923334
Characterization and expression of chalcone synthase gene from Ginkgo biloba.
Pang Y, Shen G, Wu W, Liu X, Lin J, Tan F, Sun X, Tang K., Plant Sci 168(6), 2005
PMID: IND43715164
Genome-wide investigation of light and carbon signaling interactions in Arabidopsis.
Thum KE, Shin MJ, Palenchar PM, Kouranov A, Coruzzi GM., Genome Biol 5(2), 2004
PMID: 14759260
Characterization and structural features of a chalcone synthase mutation in a white-flowering line of Matthiola incana R. Br. (Brassicaceae).
Hemleben V, Dressel A, Epping B, Lukacin R, Martens S, Austin M., Plant Mol Biol 55(3), 2004
PMID: 15604692
TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana.
Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L., Plant J 39(3), 2004
PMID: 15255866
Construction of gene expression system in hop (Humulus lupulus) lupulin gland using valerophenone synthase promoter.
Okada Y, Saeki K, Inaba A, Suda N, Kaneko T, Ito K., J Plant Physiol 160(9), 2003
PMID: 14593812
Targets of AtWRKY6 regulation during plant senescence and pathogen defense.
Robatzek S, Somssich IE., Genes Dev 16(9), 2002
PMID: 12000796
The circadian clock that controls gene expression in Arabidopsis is tissue specific.
Thain SC, Murtas G, Lynn JR, McGrath RB, Millar AJ., Plant Physiol 130(1), 2002
PMID: 12226490
Gene regulation by low level UV-B radiation: identification by DNA array analysis.
Brosché M, Schuler MA, Kalbina I, Connor L, Strid A., Photochem Photobiol Sci 1(9), 2002
PMID: 12665302
Expression of Arabidopsis LINEs from two promoters.
Ohta Y, Noma K, Tsuchimoto S, Ohtsubo E, Ohtsubo H., Plant J 32(5), 2002
PMID: 12472695
Genetic dissection of blue-light sensing in tomato using mutants deficient in cryptochrome 1 and phytochromes A, B1 and B2.
Weller JL, Perrotta G, Schreuder ME, van Tuinen A, Koornneef M, Giuliano G, Kendrick RE., Plant J 25(4), 2001
PMID: 11260499
UV and blue light signalling: pathways regulating chalcone synthase gene expression in Arabidopsis.
Jenkins GI, Long JC, Wade HK, Shenton MR, Bibikova TN., New Phytol 151(1), 2001
PMID: IND23226844
Comparative genomics and regulatory evolution: conservation and function of the Chs and Apetala3 promoters.
Koch MA, Weisshaar B, Kroymann J, Haubold B, Mitchell-Olds T., Mol Biol Evol 18(10), 2001
PMID: 11557794
UVB/UVA radiation activates a 48 kDa myelin basic protein kinase and potentiates wound signaling in tomato leaves.
Stratmann JW, Stelmach BA, Weiler EW, Ryan CA., Photochem Photobiol 71(2), 2000
PMID: 10687382
Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis.
Jin H, Cominelli E, Bailey P, Parr A, Mehrtens F, Jones J, Tonelli C, Weisshaar B, Martin C., EMBO J 19(22), 2000
PMID: 11080161
C4 GENE EXPRESSION.
Sheen J., Annu Rev Plant Physiol Plant Mol Biol 50(), 1999
PMID: 15012208
Three 4-coumarate:coenzyme A ligases in Arabidopsis thaliana represent two evolutionarily divergent classes in angiosperms.
Ehlting J, Büttner D, Wang Q, Douglas CJ, Somssich IE, Kombrink E., Plant J 19(1), 1999
PMID: 10417722
Phenylpropanoid biosynthesis and its regulation.
Weisshaar B, Jenkins GI., Curr Opin Plant Biol 1(3), 1998
PMID: 10066590
Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.
Wisman E, Hartmann U, Sagasser M, Baumann E, Palme K, Hahlbrock K, Saedler H, Weisshaar B., Proc Natl Acad Sci U S A 95(21), 1998
PMID: 9770503

45 References

Daten bereitgestellt von Europe PubMed Central.

Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor.
Dangl JL, Hauffe KD, Lipphardt S, Hahlbrock K, Scheel D., EMBO J. 6(9), 1987
PMID: 16453792
An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.
Giuliano G, Pichersky E, Malik VS, Timko MP, Scolnik PA, Cashmore AR., Proc. Natl. Acad. Sci. U.S.A. 85(19), 1988
PMID: 2902624
Regulatory elements required for light-mediated expression of the Petroselinum crispum chalcone synthase gene.
Weisshaar B, Block A, Armstrong GA, Herrmann A, Schulze-Lefert P, Hahlbrock K., Symp. Soc. Exp. Biol. 45(), 1991
PMID: 1843408
The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins.
Menkens AE, Schindler U, Cashmore AR., Trends Biochem. Sci. 20(12), 1995
PMID: 8571452
Plant bZIP proteins gather at ACGT elements.
Foster R, Izawa T, Chua NH., FASEB J. 8(2), 1994
PMID: 8119490
Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs.
Cooke R, Raynal M, Laudie M, Grellet F, Delseny M, Morris PC, Guerrier D, Giraudat J, Quigley F, Clabault G, Li YF, Mache R, Krivitzky M, Gy IJ, Kreis M, Lecharny A, Parmentier Y, Marbach J, Fleck J, Clement B, Philipps G, Herve C, Bardet C, Tremousaygue D, Hofte H., Plant J. 9(1), 1996
PMID: 8580968
Transduction of Blue-Light Signals.
Kaufman LS., Plant Physiol. 102(2), 1993
PMID: 12231824
UV and blue light signal transduction in Arabidopsis.
Jenkins GI., Plant Cell Environ. 20(6), 1997
PMID: 11541206
The "megaprimer" method of site-directed mutagenesis.
Sarkar G, Sommer SS., BioTechniques 8(4), 1990
PMID: 2340178
Two distinct cis-acting elements are involved in light-dependent activation of the pea elip promoter.
Blecken J, Weisshaar B, Herzfeld F., Mol. Gen. Genet. 245(3), 1994
PMID: 7816047
Plant homeodomain protein involved in transcriptional regulation of a pathogen defense-related gene.
Korfhage U, Trezzini GF, Meier I, Hahlbrock K, Somssich IE., Plant Cell 6(5), 1994
PMID: 7913642
Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene.
Feinbaum RL, Ausubel FM., Mol. Cell. Biol. 8(5), 1988
PMID: 3386631
Promoter elements of the mustard CHS1 gene are sufficient for light regulation in transgenic plants.
Kaiser T, Emmler K, Kretsch T, Weisshaar B, Schafer E, Batschauer A., Plant Mol. Biol. 28(2), 1995
PMID: 7599308
Inducible in vivo DNA footprints define sequences necessary for UV light activation of the parsley chalcone synthase gene.
Schulze-Lefert P, Dangl JL, Becker-Andre M, Hahlbrock K, Schulz W., EMBO J. 8(3), 1989
PMID: 2566481
Plant bZIP protein DNA binding specificity.
Izawa T, Foster R, Chua NH., J. Mol. Biol. 230(4), 1993
PMID: 8487298
Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselinum crispum.
Kawalleck P, Somssich IE, Feldbrugge M, Hahlbrock K, Weisshaar B., Plant Mol. Biol. 21(4), 1993
PMID: 8383552
Regulation of Flavonoid Biosynthetic Genes in Germinating Arabidopsis Seedlings.
Kubasek WL, Shirley BW, McKillop A, Goodman HM, Briggs W, Ausubel FM., Plant Cell 4(10), 1992
PMID: 12297632
Functional analysis of a light-responsive plant bZIP transcriptional regulator.
Feldbrugge M, Sprenger M, Dinkelbach M, Yazaki K, Harter K, Weisshaar B., Plant Cell 6(11), 1994
PMID: 7827494
The transcriptional regulator CPRF1: expression analysis and gene structure.
Feldbrugge M, Hahlbrock K, Weisshaar B., Mol. Gen. Genet. 251(6), 1996
PMID: 8757392
Functional architecture of the light-responsive chalcone synthase promoter from parsley.
Schulze-Lefert P, Becker-Andre M, Schulz W, Hahlbrock K, Dangl JL., Plant Cell 1(7), 1989
PMID: 2535519
Blue-light regulation of transcription for nuclear genes in pea.
Marrs KA, Kaufman LS., Proc. Natl. Acad. Sci. U.S.A. 86(12), 1989
PMID: 16578843
Transient expression analysis in plants using firefly luciferase reporter gene.
Luehrsen KR, de Wet JR, Walbot V., Meth. Enzymol. 216(), 1992
PMID: 1479911
Promoters from genes for plastid proteins possess regions with different sensitivities toward red and blue light.
Lubberstedt T, Bolle CE, Sopory S, Flieger K, Herrmann RG, Oelmuller R., Plant Physiol. 104(3), 1994
PMID: 8165263
Seeing blue: the discovery of cryptochrome.
Ahmad M, Cashmore AR., Plant Mol. Biol. 30(5), 1996
PMID: 8639745
MYB transcription factors in plants.
Martin C, Paz-Ares J., Trends Genet. 13(2), 1997
PMID: 9055608

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