Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy

Göhler A, Andre S, Kaltner H, Sauer M, Gabius H-J, Doose S (2010)
BIOPHYSICAL JOURNAL 98(12): 3044-3053.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Göhler, AntoniaUniBi; Andre, Sabine; Kaltner, Herbert; Sauer, MarkusUniBi; Gabius, Hans-Joachim; Doose, Soeren
Abstract / Bemerkung
Fluorescence correlation spectroscopy is applied on homologous human lectins (i.e., adhesion/growth-regulatory galectins) to detect influence of ligand binding and presence of the linker peptide in tandem-repeat-type proteins on hydrodynamic properties. Among five tested proteins, lactose binding increased the diffusion constant only in the cases of homodimeric galectin-1 and the linkerless variant of tandem-repeat-type galectin-4. To our knowledge, the close structural similarity among galectins does not translate into identical response to ligand binding. Kinetic measurements show association and dissociation rate constants in the order of 1 to 10(3) M-1 s(-1) and 10(-4) s(-1), respectively. Presence of the linker peptide in tandem-repeat-type protein leads to anomalous scaling with molecular mass. These results provide what we believe to be new insights into lectin responses to glycan binding, detectable so far only by small angle neutron scattering, and the structural relevance of the linker peptide. Methodologically, fluorescence correlation spectroscopy is shown to be a rather simple technical tool to characterize hydrodynamic properties of these proteins at a high level of sensitivity.
Erscheinungsjahr
2010
Zeitschriftentitel
BIOPHYSICAL JOURNAL
Band
98
Ausgabe
12
Seite(n)
3044-3053
ISSN
0006-3495
Page URI
https://pub.uni-bielefeld.de/record/1795332

Zitieren

Göhler A, Andre S, Kaltner H, Sauer M, Gabius H-J, Doose S. Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL. 2010;98(12):3044-3053.
Göhler, A., Andre, S., Kaltner, H., Sauer, M., Gabius, H. - J., & Doose, S. (2010). Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL, 98(12), 3044-3053. https://doi.org/10.1016/j.bpj.2010.03.040
Göhler, Antonia, Andre, Sabine, Kaltner, Herbert, Sauer, Markus, Gabius, Hans-Joachim, and Doose, Soeren. 2010. “Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy”. BIOPHYSICAL JOURNAL 98 (12): 3044-3053.
Göhler, A., Andre, S., Kaltner, H., Sauer, M., Gabius, H. - J., and Doose, S. (2010). Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL 98, 3044-3053.
Göhler, A., et al., 2010. Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL, 98(12), p 3044-3053.
A. Göhler, et al., “Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy”, BIOPHYSICAL JOURNAL, vol. 98, 2010, pp. 3044-3053.
Göhler, A., Andre, S., Kaltner, H., Sauer, M., Gabius, H.-J., Doose, S.: Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy. BIOPHYSICAL JOURNAL. 98, 3044-3053 (2010).
Göhler, Antonia, Andre, Sabine, Kaltner, Herbert, Sauer, Markus, Gabius, Hans-Joachim, and Doose, Soeren. “Hydrodynamic Properties of Human Adhesion/Growth-Regulatory Galectins Studied by Fluorescence Correlation Spectroscopy”. BIOPHYSICAL JOURNAL 98.12 (2010): 3044-3053.

25 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Exploring functional pairing between surface glycoconjugates and human galectins using programmable glycodendrimersomes.
Xiao Q, Ludwig AK, Romanò C, Buzzacchera I, Sherman SE, Vetro M, Vértesy S, Kaltner H, Reed EH, Möller M, Wilson CJ, Hammer DA, Oscarson S, Klein ML, Gabius HJ, Percec V., Proc Natl Acad Sci U S A 115(11), 2018
PMID: 29382751
Adhesion/growth-regulatory galectins tested in combination: evidence for formation of hybrids as heterodimers.
Miller MC, Ludwig AK, Wichapong K, Kaltner H, Kopitz J, Gabius HJ, Mayo KH., Biochem J 475(5), 2018
PMID: 29321242
Intra- and intermolecular interactions of human galectin-3: assessment by full-assignment-based NMR.
Ippel H, Miller MC, Vértesy S, Zheng Y, Cañada FJ, Suylen D, Umemoto K, Romanò C, Hackeng T, Tai G, Leffler H, Kopitz J, André S, Kübler D, Jiménez-Barbero J, Oscarson S, Gabius HJ, Mayo KH., Glycobiology 26(8), 2016
PMID: 26911284
Sweet complementarity: the functional pairing of glycans with lectins.
Gabius HJ, Manning JC, Kopitz J, André S, Kaltner H., Cell Mol Life Sci 73(10), 2016
PMID: 26956894
A guide into glycosciences: How chemistry, biochemistry and biology cooperate to crack the sugar code.
Solís D, Bovin NV, Davis AP, Jiménez-Barbero J, Romero A, Roy R, Smetana K, Gabius HJ., Biochim Biophys Acta 1850(1), 2015
PMID: 24685397
Dissecting molecular aspects of cell interactions using glycodendrimersomes with programmable glycan presentation and engineered human lectins.
Zhang S, Moussodia RO, Murzeau C, Sun HJ, Klein ML, Vértesy S, André S, Roy R, Gabius HJ, Percec V., Angew Chem Int Ed Engl 54(13), 2015
PMID: 25656452
Comparative lectinology: Delineating glycan-specificity profiles of the chicken galectins using neoglycoconjugates in a cell assay.
Rapoport EM, Matveeva VK, Kaltner H, André S, Vokhmyanina OA, Pazynina GV, Severov VV, Ryzhov IM, Korchagina EY, Belyanchikov IM, Gabius HJ, Bovin NV., Glycobiology 25(7), 2015
PMID: 25681326
Structural significance of galectin design: impairment of homodimer stability by linker insertion and partial reversion by ligand presence.
Vértesy S, Michalak M, Miller MC, Schnölzer M, André S, Kopitz J, Mayo KH, Gabius HJ., Protein Eng Des Sel 28(7), 2015
PMID: 25796447
Combining Crystallography and Hydrogen-Deuterium Exchange to Study Galectin-Ligand Complexes.
Ruiz FM, Gilles U, Lindner I, André S, Romero A, Reusch D, Gabius HJ., Chemistry 21(39), 2015
PMID: 26270612
Phosphorylation of multifunctional galectins by protein kinases CK1, CK2, and PKA.
Kübler D, Seidler J, André S, Kumar S, Schwartz-Albiez R, Lehmann WD, Gabius HJ., Anal Biochem 449(), 2014
PMID: 24333252
Natural single amino acid polymorphism (F19Y) in human galectin-8: detection of structural alterations and increased growth-regulatory activity on tumor cells.
Ruiz FM, Scholz BA, Buzamet E, Kopitz J, André S, Menéndez M, Romero A, Solís D, Gabius HJ., FEBS J 281(5), 2014
PMID: 24418318
Single-molecule photobleaching reveals increased MET receptor dimerization upon ligand binding in intact cells.
Dietz MS, Haße D, Ferraris DM, Göhler A, Niemann HH, Heilemann M., BMC Biophys 6(1), 2013
PMID: 23731667
Fine-tuning of prototype chicken galectins: structure of CG-2 and structure-activity correlations.
Ruiz FM, Fernández IS, López-Merino L, Lagartera L, Kaltner H, Menéndez M, André S, Solís D, Gabius HJ, Romero A., Acta Crystallogr D Biol Crystallogr 69(pt 9), 2013
PMID: 23999290
Galectin-4, a novel neuronal regulator of myelination.
Stancic M, Slijepcevic D, Nomden A, Vos MJ, de Jonge JC, Sikkema AH, Gabius HJ, Hoekstra D, Baron W., Glia 60(6), 2012
PMID: 22431161
Adhesion/growth-regulatory galectins in the human eye: localization profiles and tissue reactivities as a standard to detect disease-associated alterations.
Schlötzer-Schrehardt U, André S, Janko C, Kaltner H, Kopitz J, Gabius HJ, Herrmann M., Graefes Arch Clin Exp Ophthalmol 250(8), 2012
PMID: 22527325
Comparative study of the glycan specificities of cell-bound human tandem-repeat-type galectin-4, -8 and -9.
Vokhmyanina OA, Rapoport EM, André S, Severov VV, Ryzhov I, Pazynina GV, Korchagina E, Gabius HJ, Bovin NV., Glycobiology 22(9), 2012
PMID: 22547138
Inhibitory potential of chemical substitutions at bioinspired sites of β-D-galactopyranose on neoglycoprotein/cell surface binding of two classes of medically relevant lectins.
Giguère D, André S, Bonin MA, Bellefleur MA, Provencal A, Cloutier P, Pucci B, Roy R, Gabius HJ., Bioorg Med Chem 19(10), 2011
PMID: 21524586
Galectins as tools for glycan mapping in histology: comparison of their binding profiles to the bovine zona pellucida by confocal laser scanning microscopy.
Habermann FA, André S, Kaltner H, Kübler D, Sinowatz F, Gabius HJ., Histochem Cell Biol 135(6), 2011
PMID: 21584695
Glyco-DNA-gold nanoparticles: lectin-mediated assembly and dual-stimuli response.
Witten KG, Rech C, Eckert T, Charrak S, Richtering W, Elling L, Simon U., Small 7(14), 2011
PMID: 21656675
Sensing ligand binding to a clinically relevant lectin by tryptophan fluorescence anisotropy.
Göhler A, Büchner C, André S, Doose S, Kaltner H, Gabius HJ., Analyst 136(24), 2011
PMID: 22016878

55 References

Daten bereitgestellt von Europe PubMed Central.


AUTHOR UNKNOWN, 2009
A guide to signaling pathways connecting protein-glycan interaction with the emerging versatile effector functionality of mammalian lectins
Villalobo A., Nogales-González A., Gabius H.-J.., 2006
Glycans: bioactive signals decoded by lectins.
Gabius HJ., Biochem. Soc. Trans. 36(Pt 6), 2008
PMID: 19021582
Substitutions in the N-glycan core as regulators of biorecognition: the case of core-fucose and bisecting GlcNAc moieties.
Andre S, Kozar T, Schuberth R, Unverzagt C, Kojima S, Gabius HJ., Biochemistry 46(23), 2007
PMID: 17497937
CD4+CD7- leukemic T cells from patients with Sezary syndrome are protected from galectin-1-triggered T cell death.
Rappl G, Abken H, Muche JM, Sterry W, Tilgen W, Andre S, Kaltner H, Ugurel S, Gabius HJ, Reinhold U., Leukemia 16(5), 2002
PMID: 11986945
Determination of structural and functional overlap/divergence of five proto-type galectins by analysis of the growth-regulatory interaction with ganglioside GM1 in silico and in vitro on human neuroblastoma cells.
Andre S, Kaltner H, Lensch M, Russwurm R, Siebert HC, Fallsehr C, Tajkhorshid E, Heck AJ, von Knebel Doeberitz M, Gabius HJ, Kopitz J., Int. J. Cancer 114(1), 2005
PMID: 15523676
Tumor suppressor p16INK4a--modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells.
Andre S, Sanchez-Ruderisch H, Nakagawa H, Buchholz M, Kopitz J, Forberich P, Kemmner W, Bock C, Deguchi K, Detjen KM, Wiedenmann B, von Knebel Doeberitz M, Gress TM, Nishimura S, Rosewicz S, Gabius HJ., FEBS J. 274(13), 2007
PMID: 17535296
Glycosylation in immune cell trafficking.
Sperandio M, Gleissner CA, Ley K., Immunol. Rev. 230(1), 2009
PMID: 19594631
Animal lectins.
Gabius HJ., Eur. J. Biochem. 243(3), 1997
PMID: 9057819

AUTHOR UNKNOWN, 0
Binding and cross-linking properties of galectins.
Fred Brewer C., Biochim. Biophys. Acta 1572(2-3), 2002
PMID: 12223273
Neurobiology meets glycosciences
Ledeen R.W., Wu G.., 2009
Quaternary solution structures of galectins-1, -3, and -7.
Morris S, Ahmad N, Andre S, Kaltner H, Gabius HJ, Brenowitz M, Brewer F., Glycobiology 14(3), 2003
PMID: 14693909
Prototype chicken galectins revisited: characterization of a third protein with distinctive hydrodynamic behaviour and expression pattern in organs of adult animals.
Kaltner H, Solis D, Kopitz J, Lensch M, Lohr M, Manning JC, Murnseer M, Schnolzer M, Andre S, Saiz JL, Gabius HJ., Biochem. J. 409(2), 2008
PMID: 17887955
Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells.
Delacour D, Gouyer V, Zanetta JP, Drobecq H, Leteurtre E, Grard G, Moreau-Hannedouche O, Maes E, Pons A, Andre S, Le Bivic A, Gabius HJ, Manninen A, Simons K, Huet G., J. Cell Biol. 169(3), 2005
PMID: 15883199
Glycosylation pattern of brush border-associated glycoproteins in enterocyte-like cells: involvement of complex-type N-glycans in apical trafficking.
Morelle W, Stechly L, Andre S, Van Seuningen I, Porchet N, Gabius HJ, Michalski JC, Huet G., Biol. Chem. 390(7), 2009
PMID: 19426135
Phosphorylated human galectin-3: facile large-scale preparation of active lectin and detection of structural changes by CD spectroscopy.
Kubler D, Hung CW, Dam TK, Kopitz J, Andre S, Kaltner H, Lohr M, Manning JC, He L, Wang H, Middelberg A, Brewer CF, Reed J, Lehmann WD, Gabius HJ., Biochim. Biophys. Acta 1780(4), 2008
PMID: 18302943
Fluorescence correlation spectroscopy. II. An experimental realization.
Magde D, Elson EL, Webb WW., Biopolymers 13(1), 1974
PMID: 4818131
Fluorescence Correlation Spectroscopy: Theory and Applications
Rigler R., Elson E.S.., 2001
What fluorescence correlation spectroscopy can tell us about unfolded proteins.
Frieden C, Chattopadhyay K, Elson EL., Adv. Protein Chem. 62(), 2002
PMID: 12418102
Biological and chemical applications of fluorescence correlation spectroscopy: a review.
Hess ST, Huang S, Heikal AA, Webb WW., Biochemistry 41(3), 2002
PMID: 11790090
Fluorescence correlation spectroscopy: the technique and its applications
Krichevsky O., Bonnet G.., 2002
Fluorescence correlation spectroscopy: novel variations of an established technique.
Haustein E, Schwille P., Annu Rev Biophys Biomol Struct 36(), 2007
PMID: 17477838
The power and prospects of fluorescence microscopies and spectroscopies.
Michalet X, Kapanidis AN, Laurence T, Pinaud F, Doose S, Pflughoefft M, Weiss S., Annu Rev Biophys Biomol Struct 32(), 2003
PMID: 12598370
Fluorescent probes and bioconjugation chemistries for single-molecule fluorescence analysis of biomolecules
Kapanidis A.N., Weiss S.., 2002
Homodimeric galectin-7 (p53-induced gene 1) is a negative growth regulator for human neuroblastoma cells.
Kopitz J, Andre S, von Reitzenstein C, Versluis K, Kaltner H, Pieters RJ, Wasano K, Kuwabara I, Liu FT, Cantz M, Heck AJ, Gabius HJ., Oncogene 22(40), 2003
PMID: 13679866
New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites.
Purkrabkova T, Smetana K Jr, Dvorankova B, Holikova Z, Bock C, Lensch M, Andre S, Pytlik R, Liu FT, Klima J, Smetana K, Motlik J, Gabius HJ., Biol. Cell 95(8), 2003
PMID: 14630391
Human galectins as sensors for apoptosis/necrosis-associated surface changes of granulocytes and lymphocytes.
Beer A, Andre S, Kaltner H, Lensch M, Franz S, Sarter K, Schulze C, Gaipl US, Kern P, Herrmann M, Gabius HJ., Cytometry A 73(2), 2008
PMID: 18186087
Galectins bind to the multivalent glycoprotein asialofetuin with enhanced affinities and a gradient of decreasing binding constants.
Dam TK, Gabius HJ, Andre S, Kaltner H, Lensch M, Brewer CF., Biochemistry 44(37), 2005
PMID: 16156668
The initial step of DNA hairpin folding: a kinetic analysis using fluorescence correlation spectroscopy.
Kim J, Doose S, Neuweiler H, Sauer M., Nucleic Acids Res. 34(9), 2006
PMID: 16687657
Polymer properties of polythymine as revealed by translational diffusion.
Doose S, Barsch H, Sauer M., Biophys. J. 93(4), 2007
PMID: 17513377
Über die Veränderlichkeit der Absorptionsspektren in Lösungen und die Nebenvalenzen als ihre Ursache
Scheibe G.., 1937
Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements.
Dertinger T, Pacheco V, von der Hocht I, Hartmann R, Gregor I, Enderlein J., Chemphyschem 8(3), 2007
PMID: 17269116
Scaling Concepts in Polymer Physics
De P.G.., 1979
Unique chicken tandem-repeat-type galectin: implications of alternative splicing and a distinct expression profile compared to those of the three proto-type proteins.
Kaltner H, Solis D, Andre S, Lensch M, Manning JC, Murnseer M, Saiz JL, Gabius HJ., Biochemistry 48(20), 2009
PMID: 19344160
Calculation of hydrodynamic properties of globular proteins from their atomic-level structure.
Garcia De La Torre J, Huertas ML, Carrasco B., Biophys. J. 78(2), 2000
PMID: 10653785
The optics and performance of dual-focus fluorescence correlation spectroscopy.
Dertinger T, Loman A, Ewers B, Muller CB, Kramer B, Enderlein J., Opt Express 16(19), 2008
PMID: 18794971
Conformational differences in liganded and unliganded states of Galectin-3.
Umemoto K, Leffler H, Venot A, Valafar H, Prestegard JH., Biochemistry 42(13), 2003
PMID: 12667058
Conformational entropy changes upon lactose binding to the carbohydrate recognition domain of galectin-3.
Diehl C, Genheden S, Modig K, Ryde U, Akke M., J. Biomol. NMR 45(1-2), 2009
PMID: 19641853
Kinetics for the Life Sciences: Receptors, Transmitters and Catalysts
Gutfreund H.., 1995
Kinetics of protein-protein association explained by Brownian dynamics computer simulation.
Northrup SH, Erickson HP., Proc. Natl. Acad. Sci. U.S.A. 89(8), 1992
PMID: 1565624
Application of a Theory of Enzyme Specificity to Protein Synthesis.
Koshland DE., Proc. Natl. Acad. Sci. U.S.A. 44(2), 1958
PMID: 16590179
ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.
MONOD J, WYMAN J, CHANGEUX JP., J. Mol. Biol. 12(), 1965
PMID: 14343300
Immunohistochemical fingerprinting of the network of seven adhesion/growth-regulatory lectins in human skin and detection of distinct tumour-associated alterations.
Cada Z, Smetana K Jr, Lacina L, Plzakova Z, Stork J, Kaltner H, Russwurm R, Lensch M, Andre S, Gabius HJ., Folia Biol. (Praha) 55(4), 2009
PMID: 19691922
Slow diffusion of lactose out of galectin-3 crystals monitored by X-ray crystallography: possible implications for ligand-exchange protocols.
Collins PM, Hidari KI, Blanchard H., Acta Crystallogr. D Biol. Crystallogr. 63(Pt 3), 2007
PMID: 17327679
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 20550917
PubMed | Europe PMC

Suchen in

Google Scholar