Electron-microscopical localization of gelsolin in various crustacean muscles

Unger A, Hinssen H (2010)
CELL AND TISSUE RESEARCH 341(2): 313-323.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Gelsolin was localized by immunoelectron microscopy in fast and slow cross-striated muscles of the lobster Homarus americanus. When ultrathin sections of the muscles were labelled with anti-gelsolin and a gold-conjugated second antibody, 90% of all gold particles in the myoplasm were detected on myofibrils, preferentially in the I-band and AI-region of the sarcomeres. Both the region of the H-zone (lacking thin filaments) and the Z-disc contained no or little gold label. Under physiological conditions, a close association of gelsolin with the thin filaments was observed for both muscle types. The preferential localization of particles in the I- and AI-region indicated that gelsolin was distributed randomly over the whole length of the thin filaments. Preincubation of muscle strips with Ringer solution containing 0.5 mM EGTA resulted in a significantly different distribution pattern; gold particles were now localized preferentially in the cell periphery close to the sarcolemma, with significantly decreased abundance in the centre of the cell. Compared with the muscle under physiological conditions, the number of gold particles over sarcomeric structures was significantly reduced. Thus, binding of gelsolin to the thin filaments is apparently reversible in vivo and depends on the presence of calcium ions. We assume a functional role for gelsolin in the actin turnover processes in invertebrate muscle systems.
Stichworte
Homarus americanus (Crustacea); Gelsolin; Cross-striated muscle; Myofibril; Immunoelectron microscopy; Lobster
Erscheinungsjahr
2010
Zeitschriftentitel
CELL AND TISSUE RESEARCH
Band
341
Ausgabe
2
Seite(n)
313-323
ISSN
0302-766X
eISSN
1432-0878
Page URI
https://pub.uni-bielefeld.de/record/1794433

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Unger A, Hinssen H. Electron-microscopical localization of gelsolin in various crustacean muscles. CELL AND TISSUE RESEARCH. 2010;341(2):313-323.
Unger, A., & Hinssen, H. (2010). Electron-microscopical localization of gelsolin in various crustacean muscles. CELL AND TISSUE RESEARCH, 341(2), 313-323. doi:10.1007/s00441-010-1003-7
Unger, A., and Hinssen, H. (2010). Electron-microscopical localization of gelsolin in various crustacean muscles. CELL AND TISSUE RESEARCH 341, 313-323.
Unger, A., & Hinssen, H., 2010. Electron-microscopical localization of gelsolin in various crustacean muscles. CELL AND TISSUE RESEARCH, 341(2), p 313-323.
A. Unger and H. Hinssen, “Electron-microscopical localization of gelsolin in various crustacean muscles”, CELL AND TISSUE RESEARCH, vol. 341, 2010, pp. 313-323.
Unger, A., Hinssen, H.: Electron-microscopical localization of gelsolin in various crustacean muscles. CELL AND TISSUE RESEARCH. 341, 313-323 (2010).
Unger, Andreas, and Hinssen, Horst. “Electron-microscopical localization of gelsolin in various crustacean muscles”. CELL AND TISSUE RESEARCH 341.2 (2010): 313-323.

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PMID: 20607291
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