Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots
Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D, Kaltschmidt B, Kaltschmidt C, Heilemann M (2010)
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1803(10): 1224-1229.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Einrichtung
Centrum für Biotechnologie > Institut für Biophysik und Nanowissenschaften
Centrum für Biotechnologie > Arbeitsgruppe C. Kaltschmidt
Fakultät für Biologie > Zellbiologie der Tiere
Fakultät für Physik > AG Biomolekulare Photonik
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Centrum für Biotechnologie > Arbeitsgruppe C. Kaltschmidt
Fakultät für Biologie > Zellbiologie der Tiere
Fakultät für Physik > AG Biomolekulare Photonik
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Abstract / Bemerkung
We introduce semiconductor quantum dot-based fluorescence imaging with similar to 2-fold increased optical resolution in three dimensions as a method that allows both studying cellular structures and spatial organization of biomolecules in membranes and subcellular organelles. Target biomolecules are labelled with quantum dots via immunocytochemistry. The resolution enhancement is achieved by three-photon absorption of quantum dots and subsequent fluorescence emission from a higher-order excitonic state. Different from conventional multiphoton microscopy, this approach can be realized on any confocal microscope without the need for pulsed excitation light. We demonstrate quantum dot triexciton imaging (QDTI) of the microtubule network of U373 cells, 3D imaging of TNF receptor 2 on the plasma membrane of HeLa cells, and multicolor 3D imaging of mitochondrial cytochrome c oxidase and actin in COS-7 cells. (C) 2010 Elsevier B.V. All rights reserved.
Stichworte
Fluorescence microscopy;
Quantum dot triexciton;
imaging;
TNF receptor;
Mitochondrial inner membrane;
Superresolution
Erscheinungsjahr
2010
Zeitschriftentitel
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Band
1803
Ausgabe
10
Seite(n)
1224-1229
ISSN
0167-4889
Page URI
https://pub.uni-bielefeld.de/record/1793955
Zitieren
Heidbreder M, Endesfelder U, van de Linde S, et al. Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 2010;1803(10):1224-1229.
Heidbreder, M., Endesfelder, U., van de Linde, S., Hennig, S., Widera, D., Kaltschmidt, B., Kaltschmidt, C., et al. (2010). Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1803(10), 1224-1229. https://doi.org/10.1016/j.bbamcr.2010.06.004
Heidbreder, Meike, Endesfelder, Ulrike, van de Linde, Sebastian, Hennig, Simon, Widera, Darius, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Heilemann, Mike. 2010. “Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots”. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1803 (10): 1224-1229.
Heidbreder, M., Endesfelder, U., van de Linde, S., Hennig, S., Widera, D., Kaltschmidt, B., Kaltschmidt, C., and Heilemann, M. (2010). Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1803, 1224-1229.
Heidbreder, M., et al., 2010. Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1803(10), p 1224-1229.
M. Heidbreder, et al., “Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots”, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, vol. 1803, 2010, pp. 1224-1229.
Heidbreder, M., Endesfelder, U., van de Linde, S., Hennig, S., Widera, D., Kaltschmidt, B., Kaltschmidt, C., Heilemann, M.: Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 1803, 1224-1229 (2010).
Heidbreder, Meike, Endesfelder, Ulrike, van de Linde, Sebastian, Hennig, Simon, Widera, Darius, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Heilemann, Mike. “Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots”. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1803.10 (2010): 1224-1229.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
1 Zitation in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.
Sporbert A, Cseresnyes Z, Heidbreder M, Domaing P, Hauser S, Kaltschmidt B, Kaltschmidt C, Heilemann M, Widera D., PLoS One 8(5), 2013
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Sporbert A, Cseresnyes Z, Heidbreder M, Domaing P, Hauser S, Kaltschmidt B, Kaltschmidt C, Heilemann M, Widera D., PLoS One 8(5), 2013
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