On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y
Pasdziernik M, Kaltschmidt B, Kaltschmidt C, Klinger C, Kaufmann M (2009)
BMC Research Notes 2(1): 102.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Pasdziernik, Markus;
Kaltschmidt, BarbaraUniBi;
Kaltschmidt, ChristianUniBi;
Klinger, Claudia;
Kaufmann, Michael
Einrichtung
Abstract / Bemerkung
Background: The human cancer-related nucleoside triphosphatase (HCR-NTPase) is overexpressed in several tumour tissues including neuroblastoma. HCR-NTPase is an enzyme exhibiting a slow in vitro activity in hydrolysing nucleosidetriphosphates. However, its in vivo function is still unknown. To learn more about the physiological role of HCR-NTPase, we both overexpressed and silenced it in the neuroblastoma cell line SH-SY5Y. Findings: No effect was observed when the expression of endogenously expressed HCR-NTPase in the cells was silenced by RNA interference. On the other hand, overexpression of HCR-NTPase led to cytotoxicity of the protein in SH-SY5Y cells. Even if the catalytic essential amino acid glutamate 114 was replaced by alanine (E114A-HCR-NTPase), the protein remained cytotoxic. The results could be confirmed by successfully rescuing the cells via RNA interference. Conclusion: Although expressed in several tumours, at least in SH-SY5Y, HCR-NTPase is not essential for the cells to survive. Increased levels of the protein lead to cytotoxicity due to physical intracellular interactions rather than hydrolysis of nucleosidetriphosphates by its intrinsic residual enzymatic activity.
Erscheinungsjahr
2009
Zeitschriftentitel
BMC Research Notes
Band
2
Ausgabe
1
Seite(n)
102
ISSN
1756-0500
Page URI
https://pub.uni-bielefeld.de/record/1783164
Zitieren
Pasdziernik M, Kaltschmidt B, Kaltschmidt C, Klinger C, Kaufmann M. On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes. 2009;2(1):102.
Pasdziernik, M., Kaltschmidt, B., Kaltschmidt, C., Klinger, C., & Kaufmann, M. (2009). On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes, 2(1), 102. https://doi.org/10.1186/1756-0500-2-102
Pasdziernik, Markus, Kaltschmidt, Barbara, Kaltschmidt, Christian, Klinger, Claudia, and Kaufmann, Michael. 2009. “On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y”. BMC Research Notes 2 (1): 102.
Pasdziernik, M., Kaltschmidt, B., Kaltschmidt, C., Klinger, C., and Kaufmann, M. (2009). On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes 2, 102.
Pasdziernik, M., et al., 2009. On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes, 2(1), p 102.
M. Pasdziernik, et al., “On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y”, BMC Research Notes, vol. 2, 2009, pp. 102.
Pasdziernik, M., Kaltschmidt, B., Kaltschmidt, C., Klinger, C., Kaufmann, M.: On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes. 2, 102 (2009).
Pasdziernik, Markus, Kaltschmidt, Barbara, Kaltschmidt, Christian, Klinger, Claudia, and Kaufmann, Michael. “On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y”. BMC Research Notes 2.1 (2009): 102.
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1 Zitation in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Modeling discrete survival time using genomic feature data.
Ferber K, Archer KJ., Cancer Inform 14(suppl 2), 2015
PMID: 25861216
Ferber K, Archer KJ., Cancer Inform 14(suppl 2), 2015
PMID: 25861216
21 References
Daten bereitgestellt von Europe PubMed Central.
The cancer genome anatomy project: online resources to reveal the molecular signatures of cancer.
Strausberg RL, Buetow KH, Greenhut SF, Grouse LH, Schaefer CF., Cancer Invest. 20(7-8), 2002
PMID: 12449737
Strausberg RL, Buetow KH, Greenhut SF, Grouse LH, Schaefer CF., Cancer Invest. 20(7-8), 2002
PMID: 12449737
The Cancer Genome Anatomy Project
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
NMR structure and functional characterization of a human cancer-related nucleoside triphosphatase.
Placzek WJ, Almeida MS, Wuthrich K., J. Mol. Biol. 367(3), 2007
PMID: 17291528
Placzek WJ, Almeida MS, Wuthrich K., J. Mol. Biol. 367(3), 2007
PMID: 17291528
SOURCE: a unified genomic resource of functional annotations, ontologies, and gene expression data.
Diehn M, Sherlock G, Binkley G, Jin H, Matese JC, Hernandez-Boussard T, Rees CA, Cherry JM, Botstein D, Brown PO, Alizadeh AA., Nucleic Acids Res. 31(1), 2003
PMID: 12519986
Diehn M, Sherlock G, Binkley G, Jin H, Matese JC, Hernandez-Boussard T, Rees CA, Cherry JM, Botstein D, Brown PO, Alizadeh AA., Nucleic Acids Res. 31(1), 2003
PMID: 12519986
SOURCE search
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
Basic local alignment search tool.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ., J. Mol. Biol. 215(3), 1990
PMID: 2231712
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ., J. Mol. Biol. 215(3), 1990
PMID: 2231712
NCBI BLAST
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
The COG database: an updated version includes eukaryotes.
Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM, Mazumder R, Mekhedov SL, Nikolskaya AN, Rao BS, Smirnov S, Sverdlov AV, Vasudevan S, Wolf YI, Yin JJ, Natale DA., BMC Bioinformatics 4(), 2003
PMID: 12969510
Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM, Mazumder R, Mekhedov SL, Nikolskaya AN, Rao BS, Smirnov S, Sverdlov AV, Vasudevan S, Wolf YI, Yin JJ, Natale DA., BMC Bioinformatics 4(), 2003
PMID: 12969510
Thermophile-specific proteins: the gene product of aq_1292 from Aquifex aeolicus is an NTPase.
Klinger C, Rossbach M, Howe R, Kaufmann M., BMC Biochem. 4(), 2003
PMID: 14503925
Klinger C, Rossbach M, Howe R, Kaufmann M., BMC Biochem. 4(), 2003
PMID: 14503925
Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold.
Rossbach M, Daumke O, Klinger C, Wittinghofer A, Kaufmann M., BMC Struct. Biol. 5(), 2005
PMID: 15777481
Rossbach M, Daumke O, Klinger C, Wittinghofer A, Kaufmann M., BMC Struct. Biol. 5(), 2005
PMID: 15777481
PCOGR: phylogenetic COG ranking as an online tool to judge the specificity of COGs with respect to freely definable groups of organisms.
Meereis F, Kaufmann M., BMC Bioinformatics 5(), 2004
PMID: 15488147
Meereis F, Kaufmann M., BMC Bioinformatics 5(), 2004
PMID: 15488147
The Protein Chemistry Group·PCOGR online
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
Mining archaeal proteomes for eukaryotic proteins with novel functions: the PACE case.
Matte-Tailliez O, Zivanovic Y, Forterre P., Trends Genet. 16(12), 2000
PMID: 11102699
Matte-Tailliez O, Zivanovic Y, Forterre P., Trends Genet. 16(12), 2000
PMID: 11102699
Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones.
Biedler JL, Roffler-Tarlov S, Schachner M, Freedman LS., Cancer Res. 38(11 Pt 1), 1978
PMID: 29704
Biedler JL, Roffler-Tarlov S, Schachner M, Freedman LS., Cancer Res. 38(11 Pt 1), 1978
PMID: 29704
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC., Nature 391(6669), 1998
PMID: 9486653
Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC., Nature 391(6669), 1998
PMID: 9486653
GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity.
Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV., Mol. Biol. Evol. 21(5), 2004
PMID: 14963095
Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV., Mol. Biol. Evol. 21(5), 2004
PMID: 14963095
A novel family of P-loop NTPases with an unusual phyletic distribution and transmembrane segments inserted within the NTPase domain.
Aravind L, Iyer LM, Leipe DD, Koonin EV., Genome Biol. 5(5), 2004
PMID: 15128444
Aravind L, Iyer LM, Leipe DD, Koonin EV., Genome Biol. 5(5), 2004
PMID: 15128444
Multiple sequence alignment with the Clustal series of programs.
Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, Thompson JD., Nucleic Acids Res. 31(13), 2003
PMID: 12824352
Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, Thompson JD., Nucleic Acids Res. 31(13), 2003
PMID: 12824352
EBI Tools: ClustalW2
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
Analysis and Visualization of Genetic Variation
Nicholas KB, Nicholas HBJ, Deerfield DWI., 1997
Nicholas KB, Nicholas HBJ, Deerfield DWI., 1997
Evolution and classification of P-loop kinases and related proteins.
Leipe DD, Koonin EV, Aravind L., J. Mol. Biol. 333(4), 2003
PMID: 14568537
Leipe DD, Koonin EV, Aravind L., J. Mol. Biol. 333(4), 2003
PMID: 14568537
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