On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y
Pasdziernik M, Kaltschmidt B, Kaltschmidt C, Klinger C, Kaufmann M (2009)
BMC Research Notes 2(1): 102.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Pasdziernik, Markus;
Kaltschmidt, BarbaraUniBi;
Kaltschmidt, ChristianUniBi;
Klinger, Claudia;
Kaufmann, Michael
Einrichtung
Abstract / Bemerkung
Background: The human cancer-related nucleoside triphosphatase (HCR-NTPase) is overexpressed in several tumour tissues including neuroblastoma. HCR-NTPase is an enzyme exhibiting a slow in vitro activity in hydrolysing nucleosidetriphosphates. However, its in vivo function is still unknown. To learn more about the physiological role of HCR-NTPase, we both overexpressed and silenced it in the neuroblastoma cell line SH-SY5Y. Findings: No effect was observed when the expression of endogenously expressed HCR-NTPase in the cells was silenced by RNA interference. On the other hand, overexpression of HCR-NTPase led to cytotoxicity of the protein in SH-SY5Y cells. Even if the catalytic essential amino acid glutamate 114 was replaced by alanine (E114A-HCR-NTPase), the protein remained cytotoxic. The results could be confirmed by successfully rescuing the cells via RNA interference. Conclusion: Although expressed in several tumours, at least in SH-SY5Y, HCR-NTPase is not essential for the cells to survive. Increased levels of the protein lead to cytotoxicity due to physical intracellular interactions rather than hydrolysis of nucleosidetriphosphates by its intrinsic residual enzymatic activity.
Erscheinungsjahr
2009
Zeitschriftentitel
BMC Research Notes
Band
2
Ausgabe
1
Seite(n)
102
ISSN
1756-0500
Page URI
https://pub.uni-bielefeld.de/record/1783164
Zitieren
Pasdziernik M, Kaltschmidt B, Kaltschmidt C, Klinger C, Kaufmann M. On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes. 2009;2(1):102.
Pasdziernik, M., Kaltschmidt, B., Kaltschmidt, C., Klinger, C., & Kaufmann, M. (2009). On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes, 2(1), 102. https://doi.org/10.1186/1756-0500-2-102
Pasdziernik, Markus, Kaltschmidt, Barbara, Kaltschmidt, Christian, Klinger, Claudia, and Kaufmann, Michael. 2009. “On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y”. BMC Research Notes 2 (1): 102.
Pasdziernik, M., Kaltschmidt, B., Kaltschmidt, C., Klinger, C., and Kaufmann, M. (2009). On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes 2, 102.
Pasdziernik, M., et al., 2009. On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes, 2(1), p 102.
M. Pasdziernik, et al., “On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y”, BMC Research Notes, vol. 2, 2009, pp. 102.
Pasdziernik, M., Kaltschmidt, B., Kaltschmidt, C., Klinger, C., Kaufmann, M.: On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y. BMC Research Notes. 2, 102 (2009).
Pasdziernik, Markus, Kaltschmidt, Barbara, Kaltschmidt, Christian, Klinger, Claudia, and Kaufmann, Michael. “On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y”. BMC Research Notes 2.1 (2009): 102.
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