Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography

Riese U, Lütkemeyer D, Heidemann R, Büntemeyer H, Lehmann J (1994)
Journal of Biotechnology 34(3): 247-257.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA
Autor/in
; ; ; ;
Abstract / Bemerkung
Based on experiments in bench scale, a recycling of spent cell culture medium was performed in a 100-l pilot scale bioreactor. The cell cultivation has been done as a repeated batch procedure after the initial batch in the following four repeated batches spent medium from the previous batch was partially re-used. After microfiltration and ultrafiltration a part of the filtrate was mixed with a concentrate of amino acids and glucose, sterile filtered and subsequently filled back into the bioreactor. Up to 65% of the harvested cell- and product-free spent medium was re-used in each repeated batch. This procedure results in a saving of pure and waste water volume and saving of supplemented proteins as transferrin, insulin and lipoproteins and, therefore, also in a reduction of the production costs. A strongly acidic membrane ion exchanger was evaluated for the ability to purify the monoclonal antibodies from the pilot scale cultivation. Within minutes, gram quantities of product could be purified in a high flux system, especially developed for this purpose, achieving purities of 80%. The capacity of the acidic membrane ion exchanger was found in former investigations to be 1 mg cm -2 with recoveries up to 96%. Final purification was carried out by gel column filtration.
Stichworte
membrane ion exchanger; medium; re-use; large scale; Monoclonal antibody; economy
Erscheinungsjahr
1994
Zeitschriftentitel
Journal of Biotechnology
Band
34
Ausgabe
3
Seite(n)
247-257
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1782399

Zitieren

Riese U, Lütkemeyer D, Heidemann R, Büntemeyer H, Lehmann J. Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography. Journal of Biotechnology. 1994;34(3):247-257.
Riese, U., Lütkemeyer, D., Heidemann, R., Büntemeyer, H., & Lehmann, J. (1994). Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography. Journal of Biotechnology, 34(3), 247-257. doi:10.1016/0168-1656(94)90060-4
Riese, U., Lütkemeyer, D., Heidemann, R., Büntemeyer, H., and Lehmann, J. (1994). Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography. Journal of Biotechnology 34, 247-257.
Riese, U., et al., 1994. Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography. Journal of Biotechnology, 34(3), p 247-257.
U. Riese, et al., “Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography”, Journal of Biotechnology, vol. 34, 1994, pp. 247-257.
Riese, U., Lütkemeyer, D., Heidemann, R., Büntemeyer, H., Lehmann, J.: Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography. Journal of Biotechnology. 34, 247-257 (1994).
Riese, U., Lütkemeyer, Dirk, Heidemann, R., Büntemeyer, Heino, and Lehmann, Jürgen. “Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography”. Journal of Biotechnology 34.3 (1994): 247-257.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T08:48:46Z
MD5 Prüfsumme
059fe068571fd905ccde8f752cd6b504

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 7764958
PubMed | Europe PMC

Suchen in

Google Scholar