Optimization of serum-free fermentation processes for antibody production

Büntemeyer H, Lütkemeyer D, Lehmann J (1991)
Cytotechnology 5(1): 57-67.

Zeitschriftenaufsatz | Veröffentlicht | Deutsch
 
Download
OA
Autor*in
Abstract / Bemerkung
Serum free fermentation procedures of cell cultures have got a wide application in production of biochemicals. But, cells cultured in serum free media in general are more sensitive to changes in culture condition, especially to nutrient limitation. There are no substances from serum which can support the cells when conditions are changing. In this study special attention is directed to amino acid utilization of mouse hybridoma in batch, chemostat and perfusion fermentations. Detailed data are presented which show the considerable difference of amino acid consumption rates in different fermentation modes. Already, in batch mode there are differences of the two investigated mouse hybridoma cell lines, although they are derived from the same myeloma line. In chemostat running at a dilution rate representing maximal growth rate most of the consumption rates are significant higher than in batch. On the other hand, in perfusion mode the rates are lower than in batch. This indicates clearly the different conditions of the fermentation modes. Therefore, it is necessary to develop serum free processes under the desired production conditions. An accurate analysis of the process is strongly recommended.
Stichworte
substrate utilization; serum-free medium; hybridoma; amino acid analysis; antibody production
Erscheinungsjahr
1991
Zeitschriftentitel
Cytotechnology
Band
5
Ausgabe
1
Seite(n)
57-67
ISSN
0920-9069
eISSN
1573-0778
Page URI
https://pub.uni-bielefeld.de/record/1782361

Zitieren

Büntemeyer H, Lütkemeyer D, Lehmann J. Optimization of serum-free fermentation processes for antibody production. Cytotechnology. 1991;5(1):57-67.
Büntemeyer, H., Lütkemeyer, D., & Lehmann, J. (1991). Optimization of serum-free fermentation processes for antibody production. Cytotechnology, 5(1), 57-67. https://doi.org/10.1007/BF00365534
Büntemeyer, H., Lütkemeyer, D., and Lehmann, J. (1991). Optimization of serum-free fermentation processes for antibody production. Cytotechnology 5, 57-67.
Büntemeyer, H., Lütkemeyer, D., & Lehmann, J., 1991. Optimization of serum-free fermentation processes for antibody production. Cytotechnology, 5(1), p 57-67.
H. Büntemeyer, D. Lütkemeyer, and J. Lehmann, “Optimization of serum-free fermentation processes for antibody production”, Cytotechnology, vol. 5, 1991, pp. 57-67.
Büntemeyer, H., Lütkemeyer, D., Lehmann, J.: Optimization of serum-free fermentation processes for antibody production. Cytotechnology. 5, 57-67 (1991).
Büntemeyer, Heino, Lütkemeyer, Dirk, and Lehmann, Jürgen. “Optimization of serum-free fermentation processes for antibody production”. Cytotechnology 5.1 (1991): 57-67.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T08:48:46Z
MD5 Prüfsumme
b18757b2b63ba84a579085f69b3374aa

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 1367052
PubMed | Europe PMC

Suchen in

Google Scholar