Multiple repressor binding sites in the genome of bacteriophage P1
Velleman M, Dreiseikelmann B, Schuster H (1987)
Proceedings of the National Academy of Sciences of the United States of America 84(16): 5570-5574.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Velleman, Mathias;
Dreiseikelmann, BrigitteUniBi;
Schuster, Heinz
Abstract / Bemerkung
After digestion of bacteriophage P1 DNA with EcoRI in the presence of P1 repressor, 6 repressor binding sites were identified in 5 of 26 EcoRI fragments. Binding sites were localized by the decreased mobility of DNA fragment-repressor complexes during electrophoresis and by DNase protection ("footprinting") analysis. The repressor binding sites, or operators, comprise a 17-base-pair-long consensus sequence lacking symmetrical elements. Three operators can be related to known genes, whereas the function of the others is still unknown. The mutant P1 bac, rendering ban expression constitutive, is identified as an operator-constitutive mutation of the ban operon.
Stichworte
operator sequence and mutation;
repressor protein;
DNA cloning
Erscheinungsjahr
1987
Zeitschriftentitel
Proceedings of the National Academy of Sciences of the United States of America
Band
84
Ausgabe
16
Seite(n)
5570-5574
Page URI
https://pub.uni-bielefeld.de/record/1776704
Zitieren
Velleman M, Dreiseikelmann B, Schuster H. Multiple repressor binding sites in the genome of bacteriophage P1. Proceedings of the National Academy of Sciences of the United States of America. 1987;84(16):5570-5574.
Velleman, M., Dreiseikelmann, B., & Schuster, H. (1987). Multiple repressor binding sites in the genome of bacteriophage P1. Proceedings of the National Academy of Sciences of the United States of America, 84(16), 5570-5574.
Velleman, Mathias, Dreiseikelmann, Brigitte, and Schuster, Heinz. 1987. “Multiple repressor binding sites in the genome of bacteriophage P1”. Proceedings of the National Academy of Sciences of the United States of America 84 (16): 5570-5574.
Velleman, M., Dreiseikelmann, B., and Schuster, H. (1987). Multiple repressor binding sites in the genome of bacteriophage P1. Proceedings of the National Academy of Sciences of the United States of America 84, 5570-5574.
Velleman, M., Dreiseikelmann, B., & Schuster, H., 1987. Multiple repressor binding sites in the genome of bacteriophage P1. Proceedings of the National Academy of Sciences of the United States of America, 84(16), p 5570-5574.
M. Velleman, B. Dreiseikelmann, and H. Schuster, “Multiple repressor binding sites in the genome of bacteriophage P1”, Proceedings of the National Academy of Sciences of the United States of America, vol. 84, 1987, pp. 5570-5574.
Velleman, M., Dreiseikelmann, B., Schuster, H.: Multiple repressor binding sites in the genome of bacteriophage P1. Proceedings of the National Academy of Sciences of the United States of America. 84, 5570-5574 (1987).
Velleman, Mathias, Dreiseikelmann, Brigitte, and Schuster, Heinz. “Multiple repressor binding sites in the genome of bacteriophage P1”. Proceedings of the National Academy of Sciences of the United States of America 84.16 (1987): 5570-5574.
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