Control by pulse parameters of electric field-mediated gene transfer in mammalian cells

Wolf H, Rols MP, Boldt E, Neumann E, Teissie J (1994)
Biophysical Journal 66(2): 524-531.

Download
OA
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Volltext vorhanden für diesen Nachweis
Autor
; ; ; ;
Abstract / Bemerkung
Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses). The effect of these parameters was systematically investigated using the transient expression of the chloramphenicol acetyltransferase and the beta-galactosidase activities in Chinese hamster ovary cells. Pulsing conditions inducing reversible permeabilization of the cell plasma membrane are not sufficient to induce gene transfer. The plasmid must be present during the electric pulse if it is to be transferred across the membrane into the cytoplasm. Only the localized part of the cell membrane brought to the permeabilized state by the external field is competent. Pulse duration plays a key role in the magnitude of the transfer. The field induces a complex reaction between the membrane and the plasmid that is accumulated at the cell interface by electrophoretic forces. This leads to an insertion of the plasmid, which can then cross the membrane.
Erscheinungsjahr
Zeitschriftentitel
Biophysical Journal
Band
66
Ausgabe
2
Seite(n)
524-531
ISSN
PUB-ID

Zitieren

Wolf H, Rols MP, Boldt E, Neumann E, Teissie J. Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophysical Journal. 1994;66(2):524-531.
Wolf, H., Rols, M. P., Boldt, E., Neumann, E., & Teissie, J. (1994). Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophysical Journal, 66(2), 524-531. doi:10.1016/S0006-3495(94)80805-7
Wolf, H., Rols, M. P., Boldt, E., Neumann, E., and Teissie, J. (1994). Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophysical Journal 66, 524-531.
Wolf, H., et al., 1994. Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophysical Journal, 66(2), p 524-531.
H. Wolf, et al., “Control by pulse parameters of electric field-mediated gene transfer in mammalian cells”, Biophysical Journal, vol. 66, 1994, pp. 524-531.
Wolf, H., Rols, M.P., Boldt, E., Neumann, E., Teissie, J.: Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophysical Journal. 66, 524-531 (1994).
Wolf, Hendrik, Rols, M. P., Boldt, Elvira, Neumann, Eberhard, and Teissie, J. “Control by pulse parameters of electric field-mediated gene transfer in mammalian cells”. Biophysical Journal 66.2 (1994): 524-531.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
1970-01-01T00:00:00Z

99 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Safe and efficient novel approach for non-invasive gene electrotransfer to skin.
Pasquet L, Chabot S, Bellard E, Markelc B, Rols MP, Reynes JP, Tiraby G, Couillaud F, Teissie J, Golzio M., Sci Rep 8(1), 2018
PMID: 30443028
Combined Numerical and Experimental Investigation of Localized Electroporation-Based Cell Transfection and Sampling.
Mukherjee P, Nathamgari SSP, Kessler JA, Espinosa HD., ACS Nano 12(12), 2018
PMID: 30452236
Gene Electrotransfer: A Mechanistic Perspective.
Rosazza C, Meglic SH, Zumbusch A, Rols MP, Miklavcic D., Curr Gene Ther 16(2), 2016
PMID: 27029943
Microfluidic Screening of Electric Fields for Electroporation.
Garcia PA, Ge Z, Moran JL, Buie CR., Sci Rep 6(), 2016
PMID: 26893024
Cell Monitoring and Manipulation Systems (CMMSs) based on Glass Cell-Culture Chips (GC³s).
Buehler SM, Stubbe M, Bonk SM, Nissen M, Titipornpun K, Klinkenberg ED, Baumann W, Gimsa J., Micromachines (Basel) 7(7), 2016
PMID: 30404280
Gene therapy using plasmid DNA-encoded anti-HER2 antibody for cancers that overexpress HER2.
Kim H, Danishmalik SN, Hwang H, Sin JI, Oh J, Cho Y, Lee H, Jeong M, Kim SH, Hong HJ., Cancer Gene Ther 23(10), 2016
PMID: 27632934
Targeted electro-delivery of oligonucleotides for RNA interference: siRNA and antimiR.
Chabot S, Teissié J, Golzio M., Adv Drug Deliv Rev 81(), 2015
PMID: 24819217
Controlled gene delivery can enhance therapeutic outcome for cancer immune therapy for melanoma.
Shirley SA, Lundberg CG, Li F, Burcus N, Heller R., Curr Gene Ther 15(1), 2015
PMID: 25414028
Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation.
Venslauskas MS, Šatkauskas S., Eur Biophys J 44(5), 2015
PMID: 25939984
Protein Extraction by Means of Electroporation from E. coli with Preserved Viability.
Haberl Meglic S, Marolt T, Miklavcic D., J Membr Biol 248(5), 2015
PMID: 26201287
Scaling relationship and optimization of double-pulse electroporation.
Sadik MM, Yu M, Zheng M, Zahn JD, Shan JW, Shreiber DI, Lin H., Biophys J 106(4), 2014
PMID: 24559983
Serum influence on in-vitro gene delivery using microbubble-assisted ultrasound.
Zeghimi A, Novell A, Thépault RA, Vourc'h P, Bouakaz A, Escoffre JM., J Drug Target 22(8), 2014
PMID: 24878379
Comparison of flow cytometry, fluorescence microscopy and spectrofluorometry for analysis of gene electrotransfer efficiency.
Marjanovič I, Kandušer M, Miklavčič D, Keber MM, Pavlin M., J Membr Biol 247(12), 2014
PMID: 25146882
Pulsed electric field (PEF) as an intensification pretreatment for greener solvent lipid extraction from microalgae.
Zbinden MD, Sturm BS, Nord RD, Carey WJ, Moore D, Shinogle H, Stagg-Williams SM., Biotechnol Bioeng 110(6), 2013
PMID: 23297018
Effect of different parameters used for in vitro gene electrotransfer on gene expression efficiency, cell viability and visualization of plasmid DNA at the membrane level.
Haberl S, Kandušer M, Flisar K, Hodžić D, Bregar VB, Miklavčič D, Escoffre JM, Rols MP, Pavlin M., J Gene Med 15(5), 2013
PMID: 23564663
Electroporation mediated DNA vaccination directly to a mucosal surface results in improved immune responses.
Kichaev G, Mendoza JM, Amante D, Smith TR, McCoy JR, Sardesai NY, Broderick KE., Hum Vaccin Immunother 9(10), 2013
PMID: 23954979
Comparison of alkaline lysis with electroextraction and optimization of electric pulses to extract plasmid DNA from Escherichia coli.
Haberl S, Jarc M, Strancar A, Peterka M, Hodžić D, Miklavčič D., J Membr Biol 246(11), 2013
PMID: 23831957
Irreversible electroporation of human primary uveal melanoma in enucleated eyes.
Mandel Y, Laufer S, Belkin M, Rubinsky B, Pe'er J, Frenkel S., PLoS One 8(9), 2013
PMID: 24039721
DNA immunization.
Wang S, Lu S., Curr Protoc Microbiol 31(), 2013
PMID: 24510291
Towards the mechanisms for efficient gene transfer into cells and tissues by means of cell electroporation.
Satkauskas S, Ruzgys P, Venslauskas MS., Expert Opin Biol Ther 12(3), 2012
PMID: 22339479
Design and implementation of a microelectrode assembly for use on noncontact in situ electroporation of adherent cells.
García-Sánchez T, García-Sánchez T, Sánchez-Ortiz B, Vila I, Guitart M, Rosell J, Gómez-Foix AM, Bragós R., J Membr Biol 245(10), 2012
PMID: 22825716
Encapsulation of biomacromolecules within polymersomes by electroporation.
Wang L, Chierico L, Little D, Patikarnmonthon N, Yang Z, Azzouz M, Madsen J, Armes SP, Battaglia G., Angew Chem Int Ed Engl 51(44), 2012
PMID: 23023772
Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery.
Escoffre JM, Portet T, Favard C, Teissié J, Dean DS, Rols MP., Biochim Biophys Acta 1808(6), 2011
PMID: 21035428
Equivalent pulse parameters for electroporation.
Pucihar G, Krmelj J, Reberšek M, Napotnik TB, Miklavčič D., IEEE Trans Biomed Eng 58(11), 2011
PMID: 21900067
Electro-mediated gene transfer and expression are controlled by the life-time of DNA/membrane complex formation.
Faurie C, Rebersek M, Golzio M, Kanduser M, Escoffre JM, Pavlin M, Teissie J, Miklavcic D, Rols MP., J Gene Med 12(1), 2010
PMID: 19941315
The role of electrophoresis in gene electrotransfer.
Pavlin M, Flisar K, Kanduser M., J Membr Biol 236(1), 2010
PMID: 20640850
Analysis and comparison of electrical pulse parameters for gene electrotransfer of two different cell lines.
Marjanovic I, Haberl S, Miklavcic D, Kanduser M, Pavlin M., J Membr Biol 236(1), 2010
PMID: 20645081
Bioelectric applications for treatment of melanoma.
Beebe SJ, Schoenbach KH, Heller R., Cancers (Basel) 2(3), 2010
PMID: 24281185
Mechanisms involved in gene electrotransfer using high- and low-voltage pulses--an in vitro study.
Kanduser M, Miklavcic D, Pavlin M., Bioelectrochemistry 74(2), 2009
PMID: 18930698
What is (still not) known of the mechanism by which electroporation mediates gene transfer and expression in cells and tissues.
Escoffre JM, Portet T, Wasungu L, Teissié J, Dean D, Rols MP., Mol Biotechnol 41(3), 2009
PMID: 19016008
Gene electrotransfer: from biophysical mechanisms to in vivo applications : Part 1- Biophysical mechanisms.
Escoffre JM, Mauroy C, Portet T, Wasungu L, Rosazza C, Gilbart Y, Mallet L, Bellard E, Golzio M, Rols MP, Teissié J., Biophys Rev 1(4), 2009
PMID: 28510029
Mechanistic analysis of electroporation-induced cellular uptake of macromolecules.
Zaharoff DA, Henshaw JW, Mossop B, Yuan F., Exp Biol Med (Maywood) 233(1), 2008
PMID: 18156311
Relaxin treatment of solid tumors: effects on electric field-mediated gene delivery.
Henshaw J, Mossop B, Yuan F., Mol Cancer Ther 7(8), 2008
PMID: 18723501
Modeling electroporation in a single cell.
Krassowska W, Filev PD., Biophys J 92(2), 2007
PMID: 17056739
Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro.
Rebersek M, Faurie C, Kanduser M, Corović S, Teissié J, Rols MP, Miklavcic D., Biomed Eng Online 6(), 2007
PMID: 17601347
Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo.
Henshaw JW, Zaharoff DA, Mossop BJ, Yuan F., Bioelectrochemistry 71(2), 2007
PMID: 17728192
A numerical model of skin electropermeabilization based on in vivo experiments.
Pavselj N, Préat V, Miklavcic D., Ann Biomed Eng 35(12), 2007
PMID: 17849185
Effectiveness of tumor electrochemotherapy as a function of electric pulse strength and duration.
Satkauskas S, Batiuskaite D, Salomskaite-Davalgiene S, Venslauskas MS., Bioelectrochemistry 65(2), 2005
PMID: 15713560
Scalable encapsulation of hepatocytes by electrostatic spraying.
Zhou Y, Sun T, Chan M, Zhang J, Han Z, Wang X, Toh Y, Chen JP, Yu H., J Biotechnol 117(1), 2005
PMID: 15831251
Sequential finite element model of tissue electropermeabilization.
Sel D, Cukjati D, Batiuskaite D, Slivnik T, Mir LM, Miklavcic D., IEEE Trans Biomed Eng 52(5), 2005
PMID: 15887531
The course of tissue permeabilization studied on a mathematical model of a subcutaneous tumor in small animals.
Pavselj N, Bregar Z, Cukjati D, Batiuskaite D, Mir LM, Miklavcic D., IEEE Trans Biomed Eng 52(8), 2005
PMID: 16119232
Electrode microchamber for noninvasive perturbation of mammalian cells with nanosecond pulsed electric fields.
Sun Y, Vernier PT, Behrend M, Marcu L, Gundersen MA., IEEE Trans Nanobioscience 4(4), 2005
PMID: 16433293
Quantification of cell hybridoma yields with confocal microscopy and flow cytometry.
Gabrijel M, Repnik U, Kreft M, Grilc S, Jeras M, Zorec R., Biochem Biophys Res Commun 314(3), 2004
PMID: 14741694
Model of creation and evolution of stable electropores for DNA delivery.
Smith KC, Neu JC, Krassowska W., Biophys J 86(5), 2004
PMID: 15111399
Direct gene transfer into rat articular cartilage by in vivo electroporation.
Grossin L, Cournil-Henrionnet C, Mir LM, Liagre B, Dumas D, Etienne S, Guingamp C, Netter P, Gillet P., FASEB J 17(8), 2003
PMID: 12724342
Assessing gene and cell therapies applied in striated skeletal and cardiac muscle: is there a role for nuclear magnetic resonance?
Leroy-Willig A, Fromes Y, Paturneau-Jouas M, Carlier P., Neuromuscul Disord 13(5), 2003
PMID: 12798795
Quantitative model of small molecules uptake after in vitro cell electropermeabilization.
Puc M, Kotnik T, Mir LM, Miklavcic D., Bioelectrochemistry 60(1-2), 2003
PMID: 12893304
Cell and animal imaging of electrically mediated gene transfer.
Faurie C, Golzio M, Moller P, Teissié J, Rols MP., DNA Cell Biol 22(12), 2003
PMID: 14683588
Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach.
Dezawa M, Takano M, Negishi H, Mo X, Oshitari T, Sawada H., Micron 33(1), 2002
PMID: 11473808
Direct visualization at the single-cell level of electrically mediated gene delivery.
Golzio M, Teissie J, Rols MP., Proc Natl Acad Sci U S A 99(3), 2002
PMID: 11818537
High-efficiency gene electrotransfer into skeletal muscle: description and physiological applicability of a new pulse generator.
Durieux AC, Bonnefoy R, Manissolle C, Freyssenet D., Biochem Biophys Res Commun 296(2), 2002
PMID: 12163039
Inter-pulse interval between rectangular voltage pulses affects electroporation threshold of artificial lipid bilayers.
Lebar AM, Troiano GC, Tung L, Miklavcic D., IEEE Trans Nanobioscience 1(3), 2002
PMID: 16696301
Poly-L-glutamate, an anionic polymer, enhances transgene expression for plasmids delivered by intramuscular injection with in vivo electroporation.
Nicol F, Wong M, MacLaughlin FC, Perrard J, Wilson E, Nordstrom JL, Smith LC., Gene Ther 9(20), 2002
PMID: 12365000
Factors controlling electropermeabilisation of cell membranes.
Rols MP, Golzio M, Gabriel B, Teissié J., Technol Cancer Res Treat 1(5), 2002
PMID: 12625757
Efficient non-viral DNA-mediated gene transfer to human primary myoblasts using electroporation.
Espinos E, Liu JH, Bader CR, Bernheim L., Neuromuscul Disord 11(4), 2001
PMID: 11369185
Highly efficient electro-gene therapy of solid tumor by using an expression plasmid for the herpes simplex virus thymidine kinase gene.
Goto T, Nishi T, Tamura T, Dev SB, Takeshima H, Kochi M, Yoshizato K, Kuratsu J, Sakata T, Hofmann GA, Ushio Y., Proc Natl Acad Sci U S A 97(1), 2000
PMID: 10618422
Enhancing effect of electric stimulation on cytotoxicity of anticancer agents against rat and human glioma cells.
Horikoshi T, Naganuma H, Ohashi Y, Ueno T, Nukui H., Brain Res Bull 51(5), 2000
PMID: 10715556
Efficient DNA transfection in neuronal and astrocytic cell lines.
Ghosh C, Song W, Lahiri DK., Mol Biol Rep 27(2), 2000
PMID: 11092558
Electropermeabilization of cell membranes.
Teissié J, Eynard N, Gabriel B, Rols MP., Adv Drug Deliv Rev 35(1), 1999
PMID: 10837686
Improving electrotransfection efficiency by post-pulse centrifugation.
Li LH, Ross P, Hui SW., Gene Ther 6(3), 1999
PMID: 10435086
In vivo electroporation of skeletal muscle: threshold, efficacy and relation to electric field distribution.
Gehl J, Sorensen TH, Nielsen K, Raskmark P, Nielsen SL, Skovsgaard T, Mir LM., Biochim Biophys Acta 1428(2-3), 1999
PMID: 10434041
Dynamics of membrane sealing in transient electropermeabilization of skeletal muscle membranes.
Gowrishankar TR, Pliquett U, Lee RC., Ann N Y Acad Sci 888(), 1999
PMID: 10842634
Direct gene transfer into rat liver cells by in vivo electroporation.
Suzuki T, Shin BC, Fujikura K, Matsuzaki T, Takata K., FEBS Lett 425(3), 1998
PMID: 9563509
Control by osmotic pressure of voltage-induced permeabilization and gene transfer in mammalian cells.
Golzio M, Mora MP, Raynaud C, Delteil C, Teissié J, Rols MP., Biophys J 74(6), 1998
PMID: 9635756
The reduction in electroporation voltages by the addition of a surfactant to planar lipid bilayers.
Troiano GC, Tung L, Sharma V, Stebe KJ., Biophys J 75(2), 1998
PMID: 9675188
Gene transfer into muscle by electroporation in vivo.
Aihara H, Miyazaki J., Nat Biotechnol 16(9), 1998
PMID: 9743122
Ascorbate increases electrotransformation efficiency of intact maize cells.
Sabri N, Pelissier B, Teissie J., Anal Biochem 264(2), 1998
PMID: 9866695
In vivo gene electroinjection and expression in rat liver.
Heller R, Jaroszeski M, Atkin A, Moradpour D, Gilbert R, Wands J, Nicolau C., FEBS Lett 389(3), 1996
PMID: 8766704
Poloxamer 188 decreases susceptibility of artificial lipid membranes to electroporation.
Sharma V, Stebe K, Murphy JC, Tung L., Biophys J 71(6), 1996
PMID: 8968593
Separation of human breast cancer cells from blood by differential dielectric affinity.
Becker FF, Wang XB, Huang Y, Pethig R, Vykoukal J, Gascoyne PR., Proc Natl Acad Sci U S A 92(3), 1995
PMID: 7846067

39 References

Daten bereitgestellt von Europe PubMed Central.

Voltage-induced conductance in human erythrocyte membranes.
Kinosita K Jr, Tsong TY., Biochim. Biophys. Acta 554(2), 1979
PMID: 486454
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Birnboim HC, Doly J., Nucleic Acids Res. 7(6), 1979
PMID: 388356
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
Gorman CM, Moffat LF, Howard BH., Mol. Cell. Biol. 2(9), 1982
PMID: 6960240
Gene transfer into mouse lyoma cells by electroporation in high electric fields.
Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH., EMBO J. 1(7), 1982
PMID: 6329708
Interactions of nucleic acid double helices induced by electric field pulses.
Porschke D, Meier HJ, Ronnenberg J., Biophys. Chem. 20(3), 1984
PMID: 6498280
Formation and properties of aqueous leaks induced in human erythrocytes by electrical breakdown.
Schwister K, Deuticke B., Biochim. Biophys. Acta 816(2), 1985
PMID: 4005247
Electroporation for the efficient transfection of mammalian cells with DNA.
Chu G, Hayakawa H, Berg P., Nucleic Acids Res. 15(3), 1987
PMID: 3029703
Model of cell electrofusion. Membrane electroporation, pore coalescence and percolation.
Sugar IP, Forster W, Neumann E., Biophys. Chem. 26(2-3), 1987
PMID: 3607233
Reversible plasma membrane ultrastructural changes correlated with electropermeabilization in Chinese hamster ovary cells.
Escande-Geraud ML, Rols MP, Dupont MA, Gas N, Teissie J., Biochim. Biophys. Acta 939(2), 1988
PMID: 2451536
Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope.
Kinosita K Jr, Ashikawa I, Saita N, Yoshimura H, Itoh H, Nagayama K, Ikegami A., Biophys. J. 53(6), 1988
PMID: 3395657
Electric shock-mediated transfection of cells. Characterization and optimization of electrical parameters.
Winterbourne DJ, Thomas S, Hermon-Taylor J, Hussain I, Johnstone AP., Biochem. J. 251(2), 1988
PMID: 3401216
Passive ion permeability of lipid membranes modelled via lipid-domain interfacial area.
Cruzeiro-Hansson L, Mouritsen OG., Biochim. Biophys. Acta 944(1), 1988
PMID: 3415999
Optimization of electroporation for transfection of mammalian cell lines.
Andreason GL, Evans GA., Anal. Biochem. 180(2), 1989
PMID: 2817356
Membrane electroporation--fast molecular exchange by electroosmosis.
Dimitrov DS, Sowers AE., Biochim. Biophys. Acta 1022(3), 1990
PMID: 1690573
Effects of pulse length and pulse strength on transfection by electroporation.
Kubiniec RT, Liang H, Hui SW., BioTechniques 8(1), 1990
PMID: 2182073
High-efficiency gene transfection by in situ electroporation of cultured cells.
Zheng QA, Chang DC., Biochim. Biophys. Acta 1088(1), 1991
PMID: 1989690
High efficiency gene transfection by electroporation using a radio-frequency electric field.
Chang DC, Gao PQ, Maxwell BL., Biochim. Biophys. Acta 1092(2), 1991
PMID: 1902117
Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells.
Tekle E, Astumian RD, Chock PB., Proc. Natl. Acad. Sci. U.S.A. 88(10), 1991
PMID: 2034667
Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis.
Klenchin VA, Sukharev SI, Serov SM, Chernomordik LV, Chizmadzhev YuA ., Biophys. J. 60(4), 1991
PMID: 1660315
Fast kinetics studies of Escherichia coli electrotransformation.
Eynard N, Sixou S, Duran N, Teissie J., Eur. J. Biochem. 209(1), 1992
PMID: 1396716
Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores.
Sukharev SI, Klenchin VA, Serov SM, Chernomordik LV, Chizmadzhev YuA ., Biophys. J. 63(5), 1992
PMID: 1282374

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 8161705
PubMed | Europe PMC

Suchen in

Google Scholar