Electric field mediated gene transfer

Wong T-K, Neumann E (1982)
Biochemical and Biophysical Research Communication 107(2): 584-587.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
A simple physical chemical method of transferring genes into eucaryotic cells is described. Electric impulses in the intensity range of 5–10 kV/cm with a duration of 5–10 [mu]s were found to appreciably increase the uptake of DNA into cells. After electric field treatment, stable transformants were obtained in a system containing mouse cells deficient in thymidine kinase and a plasmid DNA harbouring the Herpes simplex thymidine kinase gene. The efficiency of transformation well compares with the results of biochemical methods of gene transfer. The electric field technique appears unique in its ease and simplicity.
Stichworte
Biophysics; Molecular biology; Biochemistry
Erscheinungsjahr
1982
Zeitschriftentitel
Biochemical and Biophysical Research Communication
Band
107
Ausgabe
2
Seite(n)
584-587
ISSN
0006-291X
Page URI
https://pub.uni-bielefeld.de/record/1774483

Zitieren

Wong T-K, Neumann E. Electric field mediated gene transfer. Biochemical and Biophysical Research Communication. 1982;107(2):584-587.
Wong, T. - K., & Neumann, E. (1982). Electric field mediated gene transfer. Biochemical and Biophysical Research Communication, 107(2), 584-587. doi:10.1016/0006-291X(82)91531-5
Wong, T. - K., and Neumann, E. (1982). Electric field mediated gene transfer. Biochemical and Biophysical Research Communication 107, 584-587.
Wong, T.-K., & Neumann, E., 1982. Electric field mediated gene transfer. Biochemical and Biophysical Research Communication, 107(2), p 584-587.
T.-K. Wong and E. Neumann, “Electric field mediated gene transfer”, Biochemical and Biophysical Research Communication, vol. 107, 1982, pp. 584-587.
Wong, T.-K., Neumann, E.: Electric field mediated gene transfer. Biochemical and Biophysical Research Communication. 107, 584-587 (1982).
Wong, Tai-Kin, and Neumann, Eberhard. “Electric field mediated gene transfer”. Biochemical and Biophysical Research Communication 107.2 (1982): 584-587.
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