Conformation changes in rRNA induced by electric impulses

Revzin A, Neumann E (1974)
Biophysical Chemistry 2(2): 144-150.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
We have investigated the effects of electric field pulses (of about 44 kV/cm magnitude and 30 [mu]sec duration) on E. coli ribosomal RNA in solution (5 x 10-4 M sodium cacodylate buffer, pH 7, 20° C). The time course of changes in ultraviolet absorbance and electric dichroism can be resolved into contributions from orientational and conformational changes. The fast part of the relaxation spectrum (completed in less than 1 [mu]s) is attributed to alignment of double-helical segments of rRNA with the external electric field; the slower part (relaxation times in the ms and [mu]s range) is shown to be due to changes in orientation of the entire rRNA molecule, concomitant with electric field-induced helix-coil transitions of oligomeric base-paired regions.
Erscheinungsjahr
1974
Zeitschriftentitel
Biophysical Chemistry
Band
2
Ausgabe
2
Seite(n)
144-150
ISSN
0301-4622
Page URI
https://pub.uni-bielefeld.de/record/1774401

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Revzin A, Neumann E. Conformation changes in rRNA induced by electric impulses. Biophysical Chemistry. 1974;2(2):144-150.
Revzin, A., & Neumann, E. (1974). Conformation changes in rRNA induced by electric impulses. Biophysical Chemistry, 2(2), 144-150. doi:10.1016/0301-4622(74)80034-7
Revzin, A., and Neumann, E. (1974). Conformation changes in rRNA induced by electric impulses. Biophysical Chemistry 2, 144-150.
Revzin, A., & Neumann, E., 1974. Conformation changes in rRNA induced by electric impulses. Biophysical Chemistry, 2(2), p 144-150.
A. Revzin and E. Neumann, “Conformation changes in rRNA induced by electric impulses”, Biophysical Chemistry, vol. 2, 1974, pp. 144-150.
Revzin, A., Neumann, E.: Conformation changes in rRNA induced by electric impulses. Biophysical Chemistry. 2, 144-150 (1974).
Revzin, Arnold, and Neumann, Eberhard. “Conformation changes in rRNA induced by electric impulses”. Biophysical Chemistry 2.2 (1974): 144-150.
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