Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix

Spodheim M, Neumann E (1977)
Biopolymers 16(2): 289-298.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Spodheim, Melanie; Neumann, EberhardUniBi
Abstract / Bemerkung
The slow kinetics of annealing processes in multistranded nucleic acids is spectrophotometrically investigated using poly(A)·2poly(U) as a model system. The absorbance changes at specific wavelengths show that double-helical (A·U) base pairs appear as transient intermediates. The annealing process is identified by the enlargement of triple-helical sequences at the cost of (A·U) base pairs and unpaired (U) residues. A large time range in the reorganization of mismatched chain configurations is characterized by a logarithmic dependence on time. This observation is quantitatively described by a kinetic model developed by Jackson. In Jackson's model the rate-limiting process in the slow annealing phase of maximizing triple-helical sequences, is the removal of strand entanglements, knots, and hairpin loops by complete unwinding of those helical stretches which stabilize the mismatched configurations. The results of the present study are briefly discussed in terms of optimum conditions for hybridization experiments and for the preparation of polynucleotide complexes commonly used to produce interferons.
Stichworte
Biophysics; Molecular biology; Biochemistry
Erscheinungsjahr
1977
Zeitschriftentitel
Biopolymers
Band
16
Ausgabe
2
Seite(n)
289-298
ISSN
0006-3525
eISSN
1097-0282
Page URI
https://pub.uni-bielefeld.de/record/1774378

Zitieren

Spodheim M, Neumann E. Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix. Biopolymers. 1977;16(2):289-298.
Spodheim, M., & Neumann, E. (1977). Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix. Biopolymers, 16(2), 289-298. https://doi.org/10.1002/bip.1977.360160206
Spodheim, Melanie, and Neumann, Eberhard. 1977. “Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix”. Biopolymers 16 (2): 289-298.
Spodheim, M., and Neumann, E. (1977). Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix. Biopolymers 16, 289-298.
Spodheim, M., & Neumann, E., 1977. Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix. Biopolymers, 16(2), p 289-298.
M. Spodheim and E. Neumann, “Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix”, Biopolymers, vol. 16, 1977, pp. 289-298.
Spodheim, M., Neumann, E.: Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix. Biopolymers. 16, 289-298 (1977).
Spodheim, Melanie, and Neumann, Eberhard. “Kinetic analysis of the annealing period in the formation of the poly(A)·2poly(U) triple helix”. Biopolymers 16.2 (1977): 289-298.
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