Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells
Leuchtenberger S, Perz A, Gatz C, Bartsch JW (2001)
Nucleic Acids Research 29(16): 76e-76.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Leuchtenberger, Stefanie;
Perz, Angela;
Gatz, Christiane;
Bartsch, Jörg W.
Einrichtung
Abstract / Bemerkung
Conditional expression of suicide genes in vivo has a wide range of applications in biological research and requires a minimal basal promoter activity in the uninduced state. To reduce basal activity of tetracycline (tc)-inducible target promoters we combined synthetic tet operators in varying numbers with a core promoter derived from the plant viral 35S promoter. An optimized promoter, P_TF, was found to exert a stringent regulation of luciferase in combination with tTA and rtTA in different mammalian cell lines. We linked P_TF to the barnase gene, coding for a highly active RNase from Bacillus amyloliquefaciens. Stable cell clones expressing barnase under control of tTA exerted cell death only after tc withdrawal, correlating with a 10-fold induction of barnase mRNA expression. Directing tTA expression through a neuron-specific enolase promoter (P_NSE) leads to barnase expression and cell death in neuronal cells after tc withdrawal. Taken together, our data demonstrate that a stringent control of barnase expression in the uninduced state improves cell ablation studies, as high frequencies of transgene propagation in both cell lines and in transgenic mice are observed.
Erscheinungsjahr
2001
Zeitschriftentitel
Nucleic Acids Research
Band
29
Ausgabe
16
Seite(n)
76e-76
ISSN
0305-7453
eISSN
1362-4962
Page URI
https://pub.uni-bielefeld.de/record/1773887
Zitieren
Leuchtenberger S, Perz A, Gatz C, Bartsch JW. Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells. Nucleic Acids Research. 2001;29(16):76e-76.
Leuchtenberger, S., Perz, A., Gatz, C., & Bartsch, J. W. (2001). Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells. Nucleic Acids Research, 29(16), 76e-76. https://doi.org/10.1093/nar/29.16.e76
Leuchtenberger, Stefanie, Perz, Angela, Gatz, Christiane, and Bartsch, Jörg W. 2001. “Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells”. Nucleic Acids Research 29 (16): 76e-76.
Leuchtenberger, S., Perz, A., Gatz, C., and Bartsch, J. W. (2001). Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells. Nucleic Acids Research 29, 76e-76.
Leuchtenberger, S., et al., 2001. Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells. Nucleic Acids Research, 29(16), p 76e-76.
S. Leuchtenberger, et al., “Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells”, Nucleic Acids Research, vol. 29, 2001, pp. 76e-76.
Leuchtenberger, S., Perz, A., Gatz, C., Bartsch, J.W.: Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells. Nucleic Acids Research. 29, 76e-76 (2001).
Leuchtenberger, Stefanie, Perz, Angela, Gatz, Christiane, and Bartsch, Jörg W. “Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells”. Nucleic Acids Research 29.16 (2001): 76e-76.
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