Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains

Giuntini E, Mengoni A, De Filippo C, Cavalieri D, Aubin-Horth N, Landry CR, Becker A, Bazzicalupo M (2005)
BMC Genomics 6(1): 158.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA
Autor/in
; ; ; ; ; ; ;
Abstract / Bemerkung
Background: Sinorhizobium meliloti is a soil bacterium that forms nitrogen-fixing nodules on the roots of leguminous plants such as alfalfa ( Medicago sativa). This species occupies different ecological niches, being present as a free-living soil bacterium and as a symbiont of plant root nodules. The genome of the type strain Rm 1021 contains one chromosome and two megaplasmids for a total genome size of 6 Mb. We applied comparative genomic hybridisation (CGH) on an oligonucleotide microarrays to estimate genetic variation at the genomic level in four natural strains, two isolated from Italian agricultural soil and two from desert soil in the Aral Sea region. Results: From 4.6 to 5.7 percent of the genes showed a pattern of hybridisation concordant with deletion, nucleotide divergence or ORF duplication when compared to the type strain Rm 1021. A large number of these polymorphisms were confirmed by sequencing and Southern blot. A statistically significant fraction of these variable genes was found on the pSymA megaplasmid and grouped in clusters. These variable genes were found to be mainly transposases or genes with unknown function. Conclusion: The obtained results allow to conclude that the symbiosis-required megaplasmid pSymA can be considered the major hot-spot for intra-specific differentiation in S. meliloti.
Erscheinungsjahr
2005
Zeitschriftentitel
BMC Genomics
Band
6
Ausgabe
1
Seite(n)
158
ISSN
1471-2164
Page URI
https://pub.uni-bielefeld.de/record/1773618

Zitieren

Giuntini E, Mengoni A, De Filippo C, et al. Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains. BMC Genomics. 2005;6(1):158.
Giuntini, E., Mengoni, A., De Filippo, C., Cavalieri, D., Aubin-Horth, N., Landry, C. R., Becker, A., et al. (2005). Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains. BMC Genomics, 6(1), 158. doi:10.1186/1471-2164-6-158
Giuntini, E., Mengoni, A., De Filippo, C., Cavalieri, D., Aubin-Horth, N., Landry, C. R., Becker, A., and Bazzicalupo, M. (2005). Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains. BMC Genomics 6, 158.
Giuntini, E., et al., 2005. Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains. BMC Genomics, 6(1), p 158.
E. Giuntini, et al., “Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains”, BMC Genomics, vol. 6, 2005, pp. 158.
Giuntini, E., Mengoni, A., De Filippo, C., Cavalieri, D., Aubin-Horth, N., Landry, C.R., Becker, A., Bazzicalupo, M.: Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains. BMC Genomics. 6, 158 (2005).
Giuntini, Elisa, Mengoni, Alessio, De Filippo, Carlotta, Cavalieri, Duccio, Aubin-Horth, Nadia, Landry, Christian R., Becker, Anke, and Bazzicalupo, Marco. “Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains”. BMC Genomics 6.1 (2005): 158.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T08:48:08Z
MD5 Prüfsumme
c517712dcb5408fdea056e5c6f860520

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 16283928
PubMed | Europe PMC

Suchen in

Google Scholar