Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence

Rögener J, Lutter P, Reinhardt R, Blüggel M, Meyer HE, Anselmetti D (2003)
Analytical chemistry 75(1): 157-159.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA
Autor/in
; ; ; ; ;
Abstract / Bemerkung
Visualization of proteins inside acrylamide and other gels usually relies on different staining methods. To omit the protein-staining procedure, we visualized unstained proteins inside acrylamide gels by laser excitation with ultraviolet (UV) light (280 nm, 35 mJ/cm²) and directly detected native UV fluorescence. In one-dimensional gels, a detection limit as low as 1 ng for bovine serum albumin and 5 ng for other proteins with a linear dynamic range (2.7 orders of magnitude) comparable to state of the art fluorescent dyes could be achieved. In addition, the application of this method to 20 µg of a whole cell lysate separated in a two-dimensional gel showed more than 600 spots. Since protein labeling always represents a serious obstacle in protein identification technologies, the working efficiency with our procedure can be considered as a significant improvement for protein visualization and reproducibility in proteomics.
Erscheinungsjahr
2003
Zeitschriftentitel
Analytical chemistry
Band
75
Ausgabe
1
Seite(n)
157-159
ISSN
0003-2700
eISSN
1520-6882
Page URI
https://pub.uni-bielefeld.de/record/1773190

Zitieren

Rögener J, Lutter P, Reinhardt R, Blüggel M, Meyer HE, Anselmetti D. Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence. Analytical chemistry. 2003;75(1):157-159.
Rögener, J., Lutter, P., Reinhardt, R., Blüggel, M., Meyer, H. E., & Anselmetti, D. (2003). Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence. Analytical chemistry, 75(1), 157-159. doi:10.1021/ac020517o
Rögener, J., Lutter, P., Reinhardt, R., Blüggel, M., Meyer, H. E., and Anselmetti, D. (2003). Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence. Analytical chemistry 75, 157-159.
Rögener, J., et al., 2003. Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence. Analytical chemistry, 75(1), p 157-159.
J. Rögener, et al., “Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence”, Analytical chemistry, vol. 75, 2003, pp. 157-159.
Rögener, J., Lutter, P., Reinhardt, R., Blüggel, M., Meyer, H.E., Anselmetti, D.: Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence. Analytical chemistry. 75, 157-159 (2003).
Rögener, Jan, Lutter, Petra, Reinhardt, Ralf, Blüggel, Martin, Meyer, Helmut E., and Anselmetti, Dario. “Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence”. Analytical chemistry 75.1 (2003): 157-159.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T08:48:07Z
MD5 Prüfsumme
1c62b51859ab2373e6c704830f41edea

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 12530833
PubMed | Europe PMC

Suchen in

Google Scholar