Size-dependent free solution DNA electrophoresis in structured microfluidic systems
Duong TT, Kim G, Ros R, Streek M, Schmid F, Brugger J, Anselmetti D, Ros A (2003)
Microelectronic Engineering 67-68(1): 905-912.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Duong, Thanh Tu;
Kim, Gyuman;
Ros, Robert;
Streek, Martin;
Schmid, Friederike;
Brugger, Jürgen;
Anselmetti, DarioUniBi 
;
Ros, Alexandra
;
Ros, AlexandraAbstract / Bemerkung
A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (< 3 µm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that [lambda]-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.
Stichworte
Fluorescence microscopy;
Single molecule;
Contact lithography;
Microchip;
Electrophoresis;
DNA
Erscheinungsjahr
2003
Zeitschriftentitel
Microelectronic Engineering
Band
67-68
Ausgabe
1
Seite(n)
905-912
ISSN
0167-9317
Page URI
https://pub.uni-bielefeld.de/record/1773175
Zitieren
Duong TT, Kim G, Ros R, et al. Size-dependent free solution DNA electrophoresis in structured microfluidic systems. Microelectronic Engineering. 2003;67-68(1):905-912.
Duong, T. T., Kim, G., Ros, R., Streek, M., Schmid, F., Brugger, J., Anselmetti, D., et al. (2003). Size-dependent free solution DNA electrophoresis in structured microfluidic systems. Microelectronic Engineering, 67-68(1), 905-912. https://doi.org/10.1016/S0167-9317(03)00153-9
Duong, Thanh Tu, Kim, Gyuman, Ros, Robert, Streek, Martin, Schmid, Friederike, Brugger, Jürgen, Anselmetti, Dario, and Ros, Alexandra. 2003. “Size-dependent free solution DNA electrophoresis in structured microfluidic systems”. Microelectronic Engineering 67-68 (1): 905-912.
Duong, T. T., Kim, G., Ros, R., Streek, M., Schmid, F., Brugger, J., Anselmetti, D., and Ros, A. (2003). Size-dependent free solution DNA electrophoresis in structured microfluidic systems. Microelectronic Engineering 67-68, 905-912.
Duong, T.T., et al., 2003. Size-dependent free solution DNA electrophoresis in structured microfluidic systems. Microelectronic Engineering, 67-68(1), p 905-912.
T.T. Duong, et al., “Size-dependent free solution DNA electrophoresis in structured microfluidic systems”, Microelectronic Engineering, vol. 67-68, 2003, pp. 905-912.
Duong, T.T., Kim, G., Ros, R., Streek, M., Schmid, F., Brugger, J., Anselmetti, D., Ros, A.: Size-dependent free solution DNA electrophoresis in structured microfluidic systems. Microelectronic Engineering. 67-68, 905-912 (2003).
Duong, Thanh Tu, Kim, Gyuman, Ros, Robert, Streek, Martin, Schmid, Friederike, Brugger, Jürgen, Anselmetti, Dario, and Ros, Alexandra. “Size-dependent free solution DNA electrophoresis in structured microfluidic systems”. Microelectronic Engineering 67-68.1 (2003): 905-912.
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