Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads

Panhorst M, Kamp P-B, Reiss G, Brückl H (2005)
Biosensors and Bioelectronics 20(8): 1685-1689.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The bondforces between biotinylated surfaces and streptavidin or avidin coated beads are investigated by a magnetic field based manipulation system for magnetic microbeads. The magnetic field is generated by currents through a set of conducting lines, and its gradient exerts a force onto the magnetic beads. The force can be increased until the bond between the bead and the surface breaks. Consistent with other groups we found two conformations for both investigated bonds. The measured bondforces for the two conformations are for Streptavidin-Biotin: 55.9 and 244.7 fN and for Avidin-Biotin: 15.9 and 58.4 fN. These very low bondforces (10-100 times smaller than earlier measurements) match to the extremely low loading rate of about 1 fN/s. This new technique thus allows to investigate biomolecular bonds by extremely low forces.
Erscheinungsjahr
2005
Zeitschriftentitel
Biosensors and Bioelectronics
Band
20
Ausgabe
8
Seite(n)
1685-1689
ISSN
0956-5663
Page URI
https://pub.uni-bielefeld.de/record/1666413

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Panhorst M, Kamp P-B, Reiss G, Brückl H. Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads. Biosensors and Bioelectronics. 2005;20(8):1685-1689.
Panhorst, M., Kamp, P. - B., Reiss, G., & Brückl, H. (2005). Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads. Biosensors and Bioelectronics, 20(8), 1685-1689. doi:10.1016/j.bios.2004.06.030
Panhorst, M., Kamp, P. - B., Reiss, G., and Brückl, H. (2005). Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads. Biosensors and Bioelectronics 20, 1685-1689.
Panhorst, M., et al., 2005. Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads. Biosensors and Bioelectronics, 20(8), p 1685-1689.
M. Panhorst, et al., “Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads”, Biosensors and Bioelectronics, vol. 20, 2005, pp. 1685-1689.
Panhorst, M., Kamp, P.-B., Reiss, G., Brückl, H.: Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads. Biosensors and Bioelectronics. 20, 1685-1689 (2005).
Panhorst, Michael, Kamp, Paul-Bertram, Reiss, Günter, and Brückl, Hubert. “Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads”. Biosensors and Bioelectronics 20.8 (2005): 1685-1689.

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PMID: 15626629
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