Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications.

Werner C, Wegener F, Unger S, Nogues S, Priault P (2009)
Rapid Commun Mass Spectrom 23(16): 2428-2438.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Werner, ChristianeUniBi ; Wegener, FrederikUniBi; Unger, StephanUniBi ; Nogues, Salvador; Priault, Pierrick
Abstract / Bemerkung
Recent advances in understanding the metabolic origin and the temporal dynamics in delta(13)C of dark-respired CO(2) (delta(13)C(res)) have led to an increasing awareness of the importance of plant isotopic fractionation in respiratory processes. Pronounced dynamics in delta(13)C(res) have been observed in a number of species and three main hypotheses have been proposed: first, diurnal changes in delta(13)C of respiratory substrates; second, post-photosynthetic discrimination in respiratory pathways; and third, dynamic decarboxylation of enriched carbon pools during the post-illumination respiration period. Since different functional groups exhibit distinct diurnal patterns in delta(13)C(res) (ranging from 0 to 10 per thousand diurnal increase), we explored these hypotheses for different ecotypes and environmental (i.e. growth light) conditions. Mass balance calculations revealed that the effect of respiratory substrates on diurnal changes in delta(13)C(res) was negligible in all investigated species. Further, rapid post-illumination changes in delta(13)C(res) (30 min), which increased from 2.6 per thousand to 5 per thousand over the course of the day, were examined by positional (13)C-labelling to quantify changes in pyruvate dehydrogenase (PDH) and Krebs cycle (KC) activity. We investigated the origin of these dynamics with Rayleigh mass balance calculations based on theoretical assumptions on fractionation processes. Neither the estimated changes of PDH and KC, nor decarboxylation of a malate pool entirely explained the observed pattern in delta(13)C(res). However, a Rayleigh fractionation of (12)C-discriminating enzymes and/or a rapid decline in the decarboxylation rate of an enriched substrate pool may explain the post-illumination peak in delta(13)C(res). These results are highly relevant since delta(13)C(res) is used in large-scale carbon cycle studies. 2009 John Wiley & Sons, Ltd.
Rapid Commun Mass Spectrom
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Werner C, Wegener F, Unger S, Nogues S, Priault P. Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications. Rapid Commun Mass Spectrom. 2009;23(16):2428-2438.
Werner, C., Wegener, F., Unger, S., Nogues, S., & Priault, P. (2009). Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications. Rapid Commun Mass Spectrom, 23(16), 2428-2438.
Werner, C., Wegener, F., Unger, S., Nogues, S., and Priault, P. (2009). Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications. Rapid Commun Mass Spectrom 23, 2428-2438.
Werner, C., et al., 2009. Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications. Rapid Commun Mass Spectrom, 23(16), p 2428-2438.
C. Werner, et al., “Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications.”, Rapid Commun Mass Spectrom, vol. 23, 2009, pp. 2428-2438.
Werner, C., Wegener, F., Unger, S., Nogues, S., Priault, P.: Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications. Rapid Commun Mass Spectrom. 23, 2428-2438 (2009).
Werner, Christiane, Wegener, Frederik, Unger, Stephan, Nogues, Salvador, and Priault, Pierrick. “Short-term dynamics of isotopic composition of leaf-respired CO2 upon darkening: measurements and implications.”. Rapid Commun Mass Spectrom 23.16 (2009): 2428-2438.

29 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.
Lehmann MM, Ghiasi S, George GM, Cormier MA, Gessler A, Saurer M, Werner RA., J Exp Bot 70(6), 2019
PMID: 30785201
Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves.
Giuliani R, Karki S, Covshoff S, Lin HC, Coe RA, Koteyeva NK, Quick WP, Von Caemmerer S, Furbank RT, Hibberd JM, Edwards GE, Cousins AB., J Exp Bot 70(10), 2019
PMID: 30840760
Changes and their possible causes in δ13C of dark-respired CO2 and its putative bulk and soluble sources during maize ontogeny.
Ghashghaie J, Badeck FW, Girardin C, Huignard C, Aydinlis Z, Fonteny C, Priault P, Fresneau C, Lamothe-Sibold M, Streb P, Terwilliger VJ., J Exp Bot 67(9), 2016
PMID: 26970389
Metabolic Fate of the Carboxyl Groups of Malate and Pyruvate and their Influence on δ(13)C of Leaf-Respired CO2 during Light Enhanced Dark Respiration.
Lehmann MM, Wegener F, Barthel M, Maurino VG, Siegwolf RT, Buchmann N, Werner C, Werner RA., Front Plant Sci 7(), 2016
PMID: 27375626
Multi-factorial in vivo stable isotope fractionation: causes, correlations, consequences and applications.
Schmidt HL, Robins RJ, Werner RA., Isotopes Environ Health Stud 51(1), 2015
PMID: 25894429
Malate as a key carbon source of leaf dark-respired CO2 across different environmental conditions in potato plants.
Lehmann MM, Rinne KT, Blessing C, Siegwolf RT, Buchmann N, Werner RA., J Exp Bot 66(19), 2015
PMID: 26139821
Phytogenic biosynthesis and emission of methyl acetate.
Jardine K, Wegener F, Abrell L, van Haren J, Werner C., Plant Cell Environ 37(2), 2014
PMID: 23862653
Two distinct plant respiratory physiotypes might exist which correspond to fast-growing and slow-growing species.
Nogués S, Aljazairi S, Arias C, Sánchez E, Aranjuelo I., J Plant Physiol 171(13), 2014
PMID: 24973588
Variability of 13C-labeling in plant leaves.
Nguyen Tu TT, Biron P, Maseyk K, Richard P, Zeller B, Quénéa K, Alexis M, Bardoux G, Vaury V, Girardin C, Pouteau V, Billiou D, Bariac T., Rapid Commun Mass Spectrom 27(17), 2013
PMID: 23939963
Rapid changes in δ¹³C of ecosystem-respired CO₂ after sunset are consistent with transient ¹³C enrichment of leaf respired CO₂.
Barbour MM, Hunt JE, Kodama N, Laubach J, McSeveny TM, Rogers GN, Tcherkez G, Wingate L., New Phytol 190(4), 2011
PMID: 21294737
δ(13) C of leaf-respired CO(2) reflects intrinsic water-use efficiency in barley.
Barbour MM, Tcherkez G, Bickford CP, Mauve C, Lamothe M, Sinton S, Brown H., Plant Cell Environ 34(5), 2011
PMID: 21276010
Metabolic fluxes, carbon isotope fractionation and respiration--lessons to be learned from plant biochemistry.
Werner RA, Buchmann N, Siegwolf RT, Kornexl BE, Gessler A., New Phytol 191(1), 2011
PMID: 21521226
Soil moisture effects on the carbon isotope composition of soil respiration.
Phillips CL, Nickerson N, Risk D, Kayler ZE, Andersen C, Mix A, Bond BJ., Rapid Commun Mass Spectrom 24(9), 2010
PMID: 20391598
On the use of phloem sap δ¹³C as an indicator of canopy carbon discrimination.
Rascher KG, Máguas C, Werner C., Tree Physiol 30(12), 2010
PMID: 21071770

48 References

Daten bereitgestellt von Europe PubMed Central.

The use of stable isotopes to study ecosystem gas exchange.
Yakir D, Sternberg LD., Oecologia 123(3), 2000
PMID: 28308584
Importance of short-term dynamics in carbon isotope ratios of ecosystem respiration (delta13C(R)) in a Mediterranean oak woodland and linkage to environmental factors.
Werner C, Unger S, Pereira JS, Maia R, David TS, Kurz-Besson C, David JS, Maguas C., New Phytol. 172(2), 2006
PMID: 16995920
Temporal dynamics of the carbon isotope composition in a Pinus sylvestris stand: from newly assimilated organic carbon to respired carbon dioxide.
Kodama N, Barnard RL, Salmon Y, Weston C, Ferrio JP, Holst J, Werner RA, Saurer M, Rennenberg H, Buchmann N, Gessler A., Oecologia 156(4), 2008
PMID: 18392642

Ghashghaie, Phytochemistry Rev. 2(), 2003

Werner, 2007
Carbon isotopes in terrestrial ecosystem pools and CO2 fluxes.
Bowling DR, Pataki DE, Randerson JT., New Phytol. 178(1), 2008
PMID: 18179603

Duranceau, Plant Cell Environ. 22(), 1999
Metabolic origin of carbon isotope composition of leaf dark-respired CO2 in French bean.
Tcherkez G, Nogues S, Bleton J, Cornic G, Badeck F, Ghashghaie J., Plant Physiol. 131(1), 2003
PMID: 12529531

Smith, Plant Cell Physiol. 12(), 1971

Ghashghaie, Plant Cell Environ. 24(), 2001
Leaf respiratory CO2 is 13C-enriched relative to leaf organic components in five species of C3 plants.
Xu CY, Lin GH, Griffin KL, Sambrotto RN., New Phytol. 163(3), 2004
PMID: IND43642165
Post-photosynthetic fractionation of stable carbon isotopes between plant organs--a widespread phenomenon.
Badeck FW, Tcherkez G, Nogues S, Piel C, Ghashghaie J., Rapid Commun. Mass Spectrom. 19(11), 2005
PMID: 15880634

Klumpp, Plant Cell Environ. 28(), 2005
Large daily variation in 13C-enrichment of leaf-respired CO2 in two Quercus forest canopies.
Hymus GJ, Maseyk K, Valentini R, Yakir D., New Phytol. 167(2), 2005
PMID: 15998391

Mortazavi, Global Biogeochem. Cycles 20(), 2006
Respiratory carbon metabolism in the high mountain plant species Ranunculus glacialis.
Nogues S, Tcherkez G, Streb P, Pardo A, Baptist F, Bligny R, Ghashghaie J, Cornic G., J. Exp. Bot. 57(14), 2006
PMID: 17030537
Diurnal variation of the delta 13C of pine needle respired CO2 evolved in darkness.
Prater JL, Mortazavi B, Chanton JP., Plant Cell Environ. 29(2), 2006
PMID: 17080636
On the metabolic origin of the carbon isotope composition of CO2 evolved from darkened light-acclimated leaves in Ricinus communis.
Gessler A, Tcherkez G, Karyanto O, Keitel C, Ferrio JP, Ghashghaie J, Kreuzwieser J, Farquhar GD., New Phytol. 181(2), 2009
PMID: 19121034
Evidence for a nonstatistical carbon isotope distribution in natural glucose.
Rossmann A, Butzenlechner M, Schmidt HL., Plant Physiol. 96(2), 1991
PMID: 16668229

Gleixner, Planta 207(), 1998
Evaluating high time-resolved changes in carbon isotope ratio of respired CO2 by a rapid in-tube incubation technique.
Werner C, Hasenbein N, Maia R, Beyschlag W, Maguas C., Rapid Commun. Mass Spectrom. 21(8), 2007
PMID: 17348086
In vivo respiratory metabolism of illuminated leaves.
Tcherkez G, Cornic G, Bligny R, Gout E, Ghashghaie J., Plant Physiol. 138(3), 2005
PMID: 15980193
A new measurement technique reveals rapid post-illumination changes in the carbon isotope composition of leaf-respired CO2.
Barbour MM, McDowell NG, Tcherkez G, Bickford CP, Hanson DT., Plant Cell Environ. 30(4), 2007
PMID: 17324233

Cornic, Physiologie Végétale 11(), 1973

Schnyder, Plant Cell Environ. 26(), 2003
Respiratory carbon metabolism following illumination in intact French bean leaves using (13)C/(12)C isotope labeling.
Nogues S, Tcherkez G, Cornic G, Ghashghaie J., Plant Physiol. 136(2), 2004
PMID: 15377781

Peperkorn, Funct. Plant Biol. 32(), 2005
Determination of the mechanism of human malic enzyme with natural and alternate dinucleotides by isotope effects.
Rishavy MA, Yang Z, Tong L, Cleland WW., Arch. Biochem. Biophys. 396(1), 2001
PMID: 11716460

McNevin, Funct. Plant Biol. 33(), 2006
Short-term changes in carbon isotope composition of soluble carbohydrates and starch: from canopy leaves to the root system.
Gottlicher S, Knohl A, Wanek W, Buchmann N, Richter A., Rapid Commun. Mass Spectrom. 20(4), 2006
PMID: 16444688
Isoprene emission from plants: why and how.
Sharkey TD, Wiberley AE, Donohue AR., Ann. Bot. 101(1), 2007
PMID: 17921528

Aranjuelo, Glob. Change Biol. 15(), 2009
Experimental evidence for diel variations of the carbon isotope composition in leaf, stem and phloem sap organic matter in Ricinus communis.
Gessler A, Tcherkez G, Peuke AD, Ghashghaie J, Farquhar GD., Plant Cell Environ. 31(7), 2008
PMID: 18331588
Respiratory metabolism of illuminated leaves depends on CO2 and O2 conditions.
Tcherkez G, Bligny R, Gout E, Mahe A, Hodges M, Cornic G., Proc. Natl. Acad. Sci. U.S.A. 105(2), 2008
PMID: 18184808

Tcherkez, Funct. Plant Biol. 32(), 2005

Atkin, Aust. J. Plant Phys. 25(), 1998

Farquhar, Annu. Rev. Plant Physiol. Plant Mol. Biol. 40(), 1989

Brugnoli, 2000
Stable carbon isotopes reveal dynamics of respiratory metabolism.
Barbour MM, Hanson DT., New Phytol. 181(2), 2009
PMID: 19121025
Seasonal, daily and diurnal variations in the stable carbon isotope composition of carbon dioxide respired by tree trunks in a deciduous oak forest.
Maunoury F, Berveiller D, Lelarge C, Pontailler JY, Vanbostal L, Damesin C., Oecologia 151(2), 2006
PMID: 17115189

Unger, Oecologia (), 2009
Does photosynthesis affect grassland soil-respired CO2 and its carbon isotope composition on a diurnal timescale?
Bahn M, Schmitt M, Siegwolf R, Richter A, Bruggemann N., New Phytol. 182(2), 2009
PMID: 19220762

Bowling, Agric. Forest. Meteorol. 118(), 2003


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