DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS

BRAND T, MILTING H, ZIPPEL M, Jockusch H (1991)
FEBS LETTERS 283(2): 289-290.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
BRAND, T; MILTING, H; ZIPPEL, M; Jockusch, HaraldUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
Dissociated cells from neonatal rat atria and ventricles were cultured in monolayers for 3 days. Newly synthesized S-35-methionine labeled myosin light chain isoforms ALC-1 ALC-2 (atrial) and VLC-1, VLC-2 (ventricular) were identified on 2D gels, and their pattern of synthesis was compared to that of myocard fragments immediately after explanatation. ALCs were synthesized in 5- to 10-fold excess over VLCs by atrial cultures, whereas the converse was true for ventricular cultures, with two exceptions: one third of the LC-1 synthesized by ventricular fragments was ALC-1, and dissociated atrial cells synthesized very little LC-2 of either isoform. The former finding corresponds to the relatively high proportion of ALC-1 in neonatal ventricular tissue. We conclude that the regional programme of LC isoform expression is basically retained after tissue explanation and even after dissociation and culturing of cardiac myocytes.
Stichworte
CELL CULTURE; 2D-ELECTROPHORESIS; PROTEIN SYNTHESIS; NEONATAL RAT
Erscheinungsjahr
1991
Zeitschriftentitel
FEBS LETTERS
Band
283
Ausgabe
2
Seite(n)
289-290
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1649830

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BRAND T, MILTING H, ZIPPEL M, Jockusch H. DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS. 1991;283(2):289-290.
BRAND, T., MILTING, H., ZIPPEL, M., & Jockusch, H. (1991). DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS, 283(2), 289-290. https://doi.org/10.1016/0014-5793(91)80610-F
BRAND, T, MILTING, H, ZIPPEL, M, and Jockusch, Harald. 1991. “DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS”. FEBS LETTERS 283 (2): 289-290.
BRAND, T., MILTING, H., ZIPPEL, M., and Jockusch, H. (1991). DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS 283, 289-290.
BRAND, T., et al., 1991. DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS, 283(2), p 289-290.
T. BRAND, et al., “DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS”, FEBS LETTERS, vol. 283, 1991, pp. 289-290.
BRAND, T., MILTING, H., ZIPPEL, M., Jockusch, H.: DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS. 283, 289-290 (1991).
BRAND, T, MILTING, H, ZIPPEL, M, and Jockusch, Harald. “DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS”. FEBS LETTERS 283.2 (1991): 289-290.

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13 References

Daten bereitgestellt von Europe PubMed Central.

Electrophoretic analysis of multiple forms of rat cardiac myosin: effects of hypophysectomy and thyroxine replacement.
Hoh JF, McGrath PA, Hale PT., J. Mol. Cell. Cardiol. 10(11), 1978
PMID: 722801
Relations between chord and slope conductances and equivalent electromotive forces.
Thompson SM., Am. J. Physiol. 250(2 Pt 1), 1986
PMID: 3953785
Isoforms of heavy and light chains of cardiac myosins from rat and rabbit.
Srihari T, Tuchschmid CR, Schaub MC., Basic Res. Cardiol. 77(6), 1982
PMID: 7159358
Expression of myosin isoenzymes in cardiac-muscle cells in culture.
Nag AC, Cheng M., Biochem. J. 221(1), 1984
PMID: 6235805
Effect of triiodothyronine on cultured neonatal rat heart cells: beating rate, myosin subunits and CK-isozymes.
Kessler-Icekson G., J. Mol. Cell. Cardiol. 20(7), 1988
PMID: 3172251
Long-term expression of isomyosins and myoendocrine functions in ectopic grafts of atrial tissue.
Jockusch H, Fuchtbauer EM, Fuchtbauer A, Leger JJ, Leger J, Maldonado CA, Forssmann WG., Proc. Natl. Acad. Sci. U.S.A. 83(19), 1986
PMID: 3463971
Sodium ion stimulates the release of atrial natriuretic polypeptides (ANP) from rat atria.
Arjamaa O, Vuolteenaho O., Biochem. Biophys. Res. Commun. 132(1), 1985
PMID: 2933035

AUTHOR UNKNOWN, 0

O'Farrell, J. Biol. Chem. 260(), 1975
Local diversity of myosin expression in mammalian atrial muscles. Variations depending on age and thyroid state in the rat and the rabbit.
Dechesne CA, Leger J, Bouvagnet P, Mairhofer H, Leger JJ., Circ. Res. 57(5), 1985
PMID: 3902278
Fiber types and myosin types in human atrial and ventricular myocardium. An anatomical description.
Bouvagnet P, Leger J, Pons F, Dechesne C, Leger JJ., Circ. Res. 55(6), 1984
PMID: 6238724
DNA synthesis in cultured adult cardiocytes.
Cantin M, Ballak M, Beuzeron-Mangina J, Anand-Srivastava MB, Tautu C., Science 214(4520), 1981
PMID: 7291996
Proto-oncogene expression in proliferating and differentiating cardiac and skeletal muscle.
Claycomb WC, Lanson NA Jr., Biochem. J. 247(3), 1987
PMID: 2447874
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