DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS

BRAND T, MILTING H, ZIPPEL M, Jockusch H (1991)
FEBS LETTERS 283(2): 289-290.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
BRAND, T; MILTING, H; ZIPPEL, M; Jockusch, HaraldUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
Dissociated cells from neonatal rat atria and ventricles were cultured in monolayers for 3 days. Newly synthesized S-35-methionine labeled myosin light chain isoforms ALC-1 ALC-2 (atrial) and VLC-1, VLC-2 (ventricular) were identified on 2D gels, and their pattern of synthesis was compared to that of myocard fragments immediately after explanatation. ALCs were synthesized in 5- to 10-fold excess over VLCs by atrial cultures, whereas the converse was true for ventricular cultures, with two exceptions: one third of the LC-1 synthesized by ventricular fragments was ALC-1, and dissociated atrial cells synthesized very little LC-2 of either isoform. The former finding corresponds to the relatively high proportion of ALC-1 in neonatal ventricular tissue. We conclude that the regional programme of LC isoform expression is basically retained after tissue explanation and even after dissociation and culturing of cardiac myocytes.
Stichworte
CELL CULTURE; 2D-ELECTROPHORESIS; PROTEIN SYNTHESIS; NEONATAL RAT
Erscheinungsjahr
1991
Zeitschriftentitel
FEBS LETTERS
Band
283
Ausgabe
2
Seite(n)
289-290
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1649830

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BRAND T, MILTING H, ZIPPEL M, Jockusch H. DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS. 1991;283(2):289-290.
BRAND, T., MILTING, H., ZIPPEL, M., & Jockusch, H. (1991). DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS, 283(2), 289-290. https://doi.org/10.1016/0014-5793(91)80610-F
BRAND, T, MILTING, H, ZIPPEL, M, and Jockusch, Harald. 1991. “DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS”. FEBS LETTERS 283 (2): 289-290.
BRAND, T., MILTING, H., ZIPPEL, M., and Jockusch, H. (1991). DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS 283, 289-290.
BRAND, T., et al., 1991. DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS, 283(2), p 289-290.
T. BRAND, et al., “DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS”, FEBS LETTERS, vol. 283, 1991, pp. 289-290.
BRAND, T., MILTING, H., ZIPPEL, M., Jockusch, H.: DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS. FEBS LETTERS. 283, 289-290 (1991).
BRAND, T, MILTING, H, ZIPPEL, M, and Jockusch, Harald. “DIFFERENTIAL SYNTHESIS BY CULTURED ATRIAL AND VENTRICULAR RAT CARDIAC MYOCYTES OF MYOSIN LIGHT CHAIN ISOFORMS”. FEBS LETTERS 283.2 (1991): 289-290.

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