2 FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN CHLORELLA-KESSLERI

Grotjohann N, HIPPE C (1993)
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES 48(7-8): 556-562.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
FPLC of crude cell extracts of Chlorella kessleri reveals two protein fractions with phosphoenolpyruvate carboxylase activity. Their mole masses, 955 kDa and 543 kDa, suggest that they are dimer and monomer of the same enzyme. Further data, however, indicate that they are more likely two different iso-forms of phosphoenolpyruvate carboxylase: Attempts to interconvert both proteins in vitro, such as through treatment with NaCl, metabolites and thiol- or histidine-group effecting reagents, were unsuccessful. The substrate affinity of the large protein was slightly higher than that of the small protein (K(m) = 1.13 and 0.76 mm, respectively); and the sensitivity to enhanced temperature was more pronounced in the large than in the smaller protein (half lifes = 23 min and 55-60 min, respectively). Some properties of both fractions, however, proved identical: 1. pH optima at pH 8.5-9, 2. Hill coefficients approx. 1, 3. no significant regulatory effect of glutamine, glutamate, aspartate and malate, 4. increase in K(m) and in Hill coefficient by citrate, and 5. identical behaviour in ion exchange chromatography. Functions and localization of the assumed two iso-forms of phosphoenolpyruvate carboxylase remain to be clarified. No specific effects of red or blue light during autotrophic growth on either protein with PEP Co activity could be found.
Stichworte
DEAE-CHROMATOGRAPHY; SUPEROSE-FPLC; CHLORELLA-KESSLERI; PHOSPHOENOLPYRUVATE CARBOXYLASE ISO-FORMS
Erscheinungsjahr
1993
Zeitschriftentitel
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
Band
48
Ausgabe
7-8
Seite(n)
556-562
ISSN
0939-5075
Page URI
https://pub.uni-bielefeld.de/record/1645307

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Grotjohann N, HIPPE C. 2 FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN CHLORELLA-KESSLERI. ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES. 1993;48(7-8):556-562.
Grotjohann, N., & HIPPE, C. (1993). 2 FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN CHLORELLA-KESSLERI. ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, 48(7-8), 556-562.
Grotjohann, N., and HIPPE, C. (1993). 2 FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN CHLORELLA-KESSLERI. ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES 48, 556-562.
Grotjohann, N., & HIPPE, C., 1993. 2 FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN CHLORELLA-KESSLERI. ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, 48(7-8), p 556-562.
N. Grotjohann and C. HIPPE, “2 FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN CHLORELLA-KESSLERI”, ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, vol. 48, 1993, pp. 556-562.
Grotjohann, N., HIPPE, C.: 2 FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN CHLORELLA-KESSLERI. ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES. 48, 556-562 (1993).
Grotjohann, Norbert, and HIPPE, C. “2 FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN CHLORELLA-KESSLERI”. ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES 48.7-8 (1993): 556-562.