THE DISULFIDE BRIDGES OF THE IMMUNOGLOBULIN-LIKE DOMAINS OF FC-GAMMA-RIIIB ARE ESSENTIAL FOR EFFICIENT EXPRESSION AND BIOLOGICAL-ACTIVITY

ENENKEL B, Frey J (1993)
JOURNAL OF PROTEIN CHEMISTRY 12(4): 459-467.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The immunoglobulin G receptor FcgammaRIIIB belongs to the immunoglobulin superfamily as two extracellular domains show homology to the immunoglobulin domains. Since some residues in these domains, such as the two cysteines, are supposed to form an intrachain disulfide bridge are so commonly conserved, they may be of importance for correct folding. Site-directed mutagenesis and expression in BHK21 confirmed this supposition for the FcgammaRIIIB. Replacing both cysteines in the first and/or second domain by serines reduced the surface expression level by 50%, whereas the ligand binding capability was 20-30% of that seen in cells expressing the wild-type receptor. Replacing one of the four cysteines resulted in the loss of surface expression. Exchanging the conserved tryptophan in the first domain by phenylalanine only slightly affected the ligand binding (25%), whereas the surface expression remained unchanged.
Stichworte
CONSERVED AMINO ACIDS; DOMAIN; STRUCTURE; IMMUNOGLOBULIN-LIKE DOMAINS; INTRACHAIN DISULFIDE BRIDGES; IMMUNOGLOBULIN-G RECEPTOR; CD16
Erscheinungsjahr
1993
Zeitschriftentitel
JOURNAL OF PROTEIN CHEMISTRY
Band
12
Ausgabe
4
Seite(n)
459-467
ISSN
0277-8033
eISSN
1573-4943
Page URI
https://pub.uni-bielefeld.de/record/1645193

Zitieren

ENENKEL B, Frey J. THE DISULFIDE BRIDGES OF THE IMMUNOGLOBULIN-LIKE DOMAINS OF FC-GAMMA-RIIIB ARE ESSENTIAL FOR EFFICIENT EXPRESSION AND BIOLOGICAL-ACTIVITY. JOURNAL OF PROTEIN CHEMISTRY. 1993;12(4):459-467.
ENENKEL, B., & Frey, J. (1993). THE DISULFIDE BRIDGES OF THE IMMUNOGLOBULIN-LIKE DOMAINS OF FC-GAMMA-RIIIB ARE ESSENTIAL FOR EFFICIENT EXPRESSION AND BIOLOGICAL-ACTIVITY. JOURNAL OF PROTEIN CHEMISTRY, 12(4), 459-467. doi:10.1007/BF01025046
ENENKEL, B., and Frey, J. (1993). THE DISULFIDE BRIDGES OF THE IMMUNOGLOBULIN-LIKE DOMAINS OF FC-GAMMA-RIIIB ARE ESSENTIAL FOR EFFICIENT EXPRESSION AND BIOLOGICAL-ACTIVITY. JOURNAL OF PROTEIN CHEMISTRY 12, 459-467.
ENENKEL, B., & Frey, J., 1993. THE DISULFIDE BRIDGES OF THE IMMUNOGLOBULIN-LIKE DOMAINS OF FC-GAMMA-RIIIB ARE ESSENTIAL FOR EFFICIENT EXPRESSION AND BIOLOGICAL-ACTIVITY. JOURNAL OF PROTEIN CHEMISTRY, 12(4), p 459-467.
B. ENENKEL and J. Frey, “THE DISULFIDE BRIDGES OF THE IMMUNOGLOBULIN-LIKE DOMAINS OF FC-GAMMA-RIIIB ARE ESSENTIAL FOR EFFICIENT EXPRESSION AND BIOLOGICAL-ACTIVITY”, JOURNAL OF PROTEIN CHEMISTRY, vol. 12, 1993, pp. 459-467.
ENENKEL, B., Frey, J.: THE DISULFIDE BRIDGES OF THE IMMUNOGLOBULIN-LIKE DOMAINS OF FC-GAMMA-RIIIB ARE ESSENTIAL FOR EFFICIENT EXPRESSION AND BIOLOGICAL-ACTIVITY. JOURNAL OF PROTEIN CHEMISTRY. 12, 459-467 (1993).
ENENKEL, B, and Frey, Jürgen. “THE DISULFIDE BRIDGES OF THE IMMUNOGLOBULIN-LIKE DOMAINS OF FC-GAMMA-RIIIB ARE ESSENTIAL FOR EFFICIENT EXPRESSION AND BIOLOGICAL-ACTIVITY”. JOURNAL OF PROTEIN CHEMISTRY 12.4 (1993): 459-467.

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