DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN

KNAUPER V, WILHELM SM, SEPERACK PK, DECLERCK YA, LANGLEY KE, OSTHUES A, Tschesche H (1993)
BIOCHEMICAL JOURNAL 295: 581-586.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Human neutrophil procollagenase was activated by incubation with recombinant active stromelysin. Activation was achieved by cleavage of the Gly78-Phe79 peptide bond at the end of the propeptide domain in a single-step activation mechanism. In addition, accelerated activation was achieved when N-terminally truncated, latent collagenase (with Phe49 as its N-terminal residue) was incubated with recombinant active stromelysin. Determination of the specific activity of recombinant-stromelysin-activated neutrophil collagenase with dinitrophenyl-octapeptide or type I collagen demonstrated the generation of high specific activity. The specific activity of stromelysin-activated enzyme was considerably higher than that of trypsin- or HgCl2-activated collagenase. Thus human neutrophil collagenase is superactivated, like the homologous fibroblast collagenase [Murphy, Cockett, Stephens, Smith and Docherty (1987) Biochem. J. 248, 265-268]. The occurrence of Phe79 at the N-terminus of the neutrophil collagenase seemed to be critical for superactivation, which is in agreement with data published Suzuki. Enghild, Morodomi, Salvesen and Nagase [(1990) Biochemistry 29. 10261-10270] on fibroblast collagenase.
Erscheinungsjahr
1993
Zeitschriftentitel
BIOCHEMICAL JOURNAL
Band
295
Seite(n)
581-586
ISSN
0264-6021
Page URI
https://pub.uni-bielefeld.de/record/1645008

Zitieren

KNAUPER V, WILHELM SM, SEPERACK PK, et al. DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL. 1993;295:581-586.
KNAUPER, V., WILHELM, S. M., SEPERACK, P. K., DECLERCK, Y. A., LANGLEY, K. E., OSTHUES, A., & Tschesche, H. (1993). DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL, 295, 581-586.
KNAUPER, V., WILHELM, S. M., SEPERACK, P. K., DECLERCK, Y. A., LANGLEY, K. E., OSTHUES, A., and Tschesche, H. (1993). DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL 295, 581-586.
KNAUPER, V., et al., 1993. DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL, 295, p 581-586.
V. KNAUPER, et al., “DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN”, BIOCHEMICAL JOURNAL, vol. 295, 1993, pp. 581-586.
KNAUPER, V., WILHELM, S.M., SEPERACK, P.K., DECLERCK, Y.A., LANGLEY, K.E., OSTHUES, A., Tschesche, H.: DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL. 295, 581-586 (1993).
KNAUPER, V, WILHELM, SM, SEPERACK, PK, DECLERCK, YA, LANGLEY, KE, OSTHUES, A, and Tschesche, Harald. “DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN”. BIOCHEMICAL JOURNAL 295 (1993): 581-586.

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