SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION
STEINMANN D, WIGGERICH HG, KLAUKE B, SCHRAMM U, Pühler A, PRIEFER UB (1993)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40(2-3): 356-360.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
STEINMANN, D;
WIGGERICH, HG;
KLAUKE, B;
SCHRAMM, U;
Pühler, AlfredUniBi 
;
PRIEFER, UB
;
PRIEFER, UBEinrichtung
Abstract / Bemerkung
A system allowing transposon mutagenesis of cloned DNA fragments in Escherichia coil with Tn4431, which carries the promotorless luciferase (lux) operon of Vibrio fischeri, has been developed. The transposon delivery plasmid, pDS1, based on an IncF replicon, is thermosensitive in replication and mobilizable to many Gram-negative bacteria. We used pDS1 for Tn4431-saturation mutagenesis of a 10-kb DNA fragment of Xanthomonas campestris pv. campestris (X.c.c.) in E. coli and showed that the expression of the lux operon was dependent on orientation and location of the transposon. Transfer of a specific Tn4431 insertion to X.c.c. allowed the determination of the bioluminescence phenotype in planta.
Erscheinungsjahr
1993
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
40
Ausgabe
2-3
Seite(n)
356-360
ISSN
0175-7598
Page URI
https://pub.uni-bielefeld.de/record/1644905
Zitieren
STEINMANN D, WIGGERICH HG, KLAUKE B, SCHRAMM U, Pühler A, PRIEFER UB. SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1993;40(2-3):356-360.
STEINMANN, D., WIGGERICH, H. G., KLAUKE, B., SCHRAMM, U., Pühler, A., & PRIEFER, U. B. (1993). SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 40(2-3), 356-360.
STEINMANN, D, WIGGERICH, HG, KLAUKE, B, SCHRAMM, U, Pühler, Alfred, and PRIEFER, UB. 1993. “SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40 (2-3): 356-360.
STEINMANN, D., WIGGERICH, H. G., KLAUKE, B., SCHRAMM, U., Pühler, A., and PRIEFER, U. B. (1993). SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40, 356-360.
STEINMANN, D., et al., 1993. SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 40(2-3), p 356-360.
D. STEINMANN, et al., “SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 40, 1993, pp. 356-360.
STEINMANN, D., WIGGERICH, H.G., KLAUKE, B., SCHRAMM, U., Pühler, A., PRIEFER, U.B.: SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 40, 356-360 (1993).
STEINMANN, D, WIGGERICH, HG, KLAUKE, B, SCHRAMM, U, Pühler, Alfred, and PRIEFER, UB. “SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40.2-3 (1993): 356-360.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
3 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon.
Katzen F, Becker A, Ielmini MV, Oddo CG, Ielpi L., Appl Environ Microbiol 65(1), 1999
PMID: 9872790
Katzen F, Becker A, Ielmini MV, Oddo CG, Ielpi L., Appl Environ Microbiol 65(1), 1999
PMID: 9872790
Unusual structure of the tonB-exb DNA region of Xanthomonas campestris pv. campestris: tonB, exbB, and exbD1 are essential for ferric iron uptake, but exbD2 is not.
Wiggerich HG, Klauke B, Köplin R, Priefer UB, Pühler A., J Bacteriol 179(22), 1997
PMID: 9371459
Wiggerich HG, Klauke B, Köplin R, Priefer UB, Pühler A., J Bacteriol 179(22), 1997
PMID: 9371459
Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.
Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L., J Bacteriol 178(14), 1996
PMID: 8763965
Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L., J Bacteriol 178(14), 1996
PMID: 8763965
12 References
Daten bereitgestellt von Europe PubMed Central.
Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.
Kleckner N, Barker DF, Ross DG, Botstein D., Genetics 90(3), 1978
PMID: 365678
Kleckner N, Barker DF, Ross DG, Botstein D., Genetics 90(3), 1978
PMID: 365678
Tissue-specific and stress-enhancing expression of the TR promoter for mannopine synthase in transgenic medicinal plants.
Saito K, Yamazaki M, Kaneko H, Murakoshi I, Fukuda Y, Van Montagu M., Planta 184(1), 1991
PMID: 24193927
Saito K, Yamazaki M, Kaneko H, Murakoshi I, Fukuda Y, Van Montagu M., Planta 184(1), 1991
PMID: 24193927
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
Boyer HW, Roulland-Dussoix D., J. Mol. Biol. 41(3), 1969
PMID: 4896022
Boyer HW, Roulland-Dussoix D., J. Mol. Biol. 41(3), 1969
PMID: 4896022
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Vieira J, Messing J., Gene 19(3), 1982
PMID: 6295879
Vieira J, Messing J., Gene 19(3), 1982
PMID: 6295879
Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris.
Hotte B, Rath-Arnold I, Puhler A, Simon R., J. Bacteriol. 172(5), 1990
PMID: 2332409
Hotte B, Rath-Arnold I, Puhler A, Simon R., J. Bacteriol. 172(5), 1990
PMID: 2332409
Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria.
Simon R, O'Connell M, Labes M, Puhler A., Meth. Enzymol. 118(), 1986
PMID: 3005803
Simon R, O'Connell M, Labes M, Puhler A., Meth. Enzymol. 118(), 1986
PMID: 3005803
Construction of plasmids carrying the cI gene of bacteriophage lambda.
Backman K, Ptashne M, Gilbert W., Proc. Natl. Acad. Sci. U.S.A. 73(11), 1976
PMID: 1069307
Backman K, Ptashne M, Gilbert W., Proc. Natl. Acad. Sci. U.S.A. 73(11), 1976
PMID: 1069307
Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri.
Engebrecht J, Nealson K, Silverman M., Cell 32(3), 1983
PMID: 6831560
Engebrecht J, Nealson K, Silverman M., Cell 32(3), 1983
PMID: 6831560
A plant-inducible gene of Xanthomonas campestris pv. campestris encodes an exocellular component required for growth in the host and hypersensitivity on nonhosts.
Kamoun S, Kado CI., J. Bacteriol. 172(9), 1990
PMID: 2168373
Kamoun S, Kado CI., J. Bacteriol. 172(9), 1990
PMID: 2168373
A family of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing.
Arnold W, Puhler A., Gene 70(1), 1988
PMID: 2907323
Arnold W, Puhler A., Gene 70(1), 1988
PMID: 2907323
Mutants of the mini-F plasmid pML31 thermosensitive in replication.
Eichenlaub R., J. Bacteriol. 138(2), 1979
PMID: 374396
Eichenlaub R., J. Bacteriol. 138(2), 1979
PMID: 374396
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 7764391
PubMed | Europe PMC
Suchen in
Google Scholar