SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION
STEINMANN D, WIGGERICH HG, KLAUKE B, SCHRAMM U, Pühler A, PRIEFER UB (1993)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40(2-3): 356-360.
Zeitschriftenaufsatz
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Autor*in
STEINMANN, D;
WIGGERICH, HG;
KLAUKE, B;
SCHRAMM, U;
Pühler, AlfredUniBi 
;
PRIEFER, UB
;
PRIEFER, UBEinrichtung
Abstract / Bemerkung
A system allowing transposon mutagenesis of cloned DNA fragments in Escherichia coil with Tn4431, which carries the promotorless luciferase (lux) operon of Vibrio fischeri, has been developed. The transposon delivery plasmid, pDS1, based on an IncF replicon, is thermosensitive in replication and mobilizable to many Gram-negative bacteria. We used pDS1 for Tn4431-saturation mutagenesis of a 10-kb DNA fragment of Xanthomonas campestris pv. campestris (X.c.c.) in E. coli and showed that the expression of the lux operon was dependent on orientation and location of the transposon. Transfer of a specific Tn4431 insertion to X.c.c. allowed the determination of the bioluminescence phenotype in planta.
Erscheinungsjahr
1993
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
40
Ausgabe
2-3
Seite(n)
356-360
ISSN
0175-7598
Page URI
https://pub.uni-bielefeld.de/record/1644905
Zitieren
STEINMANN D, WIGGERICH HG, KLAUKE B, SCHRAMM U, Pühler A, PRIEFER UB. SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1993;40(2-3):356-360.
STEINMANN, D., WIGGERICH, H. G., KLAUKE, B., SCHRAMM, U., Pühler, A., & PRIEFER, U. B. (1993). SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 40(2-3), 356-360.
STEINMANN, D, WIGGERICH, HG, KLAUKE, B, SCHRAMM, U, Pühler, Alfred, and PRIEFER, UB. 1993. “SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40 (2-3): 356-360.
STEINMANN, D., WIGGERICH, H. G., KLAUKE, B., SCHRAMM, U., Pühler, A., and PRIEFER, U. B. (1993). SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40, 356-360.
STEINMANN, D., et al., 1993. SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 40(2-3), p 356-360.
D. STEINMANN, et al., “SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 40, 1993, pp. 356-360.
STEINMANN, D., WIGGERICH, H.G., KLAUKE, B., SCHRAMM, U., Pühler, A., PRIEFER, U.B.: SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 40, 356-360 (1993).
STEINMANN, D, WIGGERICH, HG, KLAUKE, B, SCHRAMM, U, Pühler, Alfred, and PRIEFER, UB. “SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40.2-3 (1993): 356-360.
3 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon.
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Unusual structure of the tonB-exb DNA region of Xanthomonas campestris pv. campestris: tonB, exbB, and exbD1 are essential for ferric iron uptake, but exbD2 is not.
Wiggerich HG, Klauke B, Köplin R, Priefer UB, Pühler A., J Bacteriol 179(22), 1997
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Wiggerich HG, Klauke B, Köplin R, Priefer UB, Pühler A., J Bacteriol 179(22), 1997
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Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L., J Bacteriol 178(14), 1996
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Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L., J Bacteriol 178(14), 1996
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