RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT

GIEFFERS C, ENGELHARDT W, BRENZEL G, MATSUISHI T, Frey J (1993)
EUROPEAN JOURNAL OF CELL BIOLOGY 62(2): 352-361.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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GIEFFERS, C; ENGELHARDT, W; BRENZEL, G; MATSUISHI, T; Frey, JürgenUniBi
Abstract / Bemerkung
OSF-1/HB-GAM is a member of developmentally regulated growth factors and cytokines. High expression levels of this factor are found in different tissues, e.g., in brain and in bone. We have analyzed the biological function and binding properties of natural OSF-1 to human osteoblasts. Using antibodies raised against the entire OSF-1 molecule or a synthetic carboxy-terminal peptide (amino acids (aa) 110-140) we have investigated the binding sites of rat OSF-1 on human osteoblast-like osteosarcoma cell lines: HOS(TE85) and MG-63. Immunofluorescence microscopic studies and flow cytometric data revealed that OSF-1 is specifically bound to the surface of these cells. Further characterization of the binding sites showed that both osteosarcoma cells express two different kinds of binding sites: Besides binding to a specific OSF-1 receptor, OSF-1 also significantly binds to cell surface heparan sulfates. Using the peptide specific polyclonal antibody we show that the carboxy-terminal domain, aa 110 to 140 of OSF-1, seems to be involved in ligand binding. Studies on the biological function of OSF-1 revealed a strong cell attachment promoting activity in vitro. This activity is not diminished after digestion of cell surface heparan sulfates by heparinase I and heparinase I, demonstrating that the OSF-1 receptor mediates the cell attachment of osteoblasts. Our results indicate that one biological function of OSF-1 is the promotion of osteoblast attachment to the extracellular bone matrix.
Stichworte
RECEPTOR BINDING; OSF-1/HB-GAM; OSTEOBLAST; PROTEOGLYCAN
Erscheinungsjahr
1993
Zeitschriftentitel
EUROPEAN JOURNAL OF CELL BIOLOGY
Band
62
Ausgabe
2
Seite(n)
352-361
ISSN
0171-9335
Page URI
https://pub.uni-bielefeld.de/record/1644673

Zitieren

GIEFFERS C, ENGELHARDT W, BRENZEL G, MATSUISHI T, Frey J. RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT. EUROPEAN JOURNAL OF CELL BIOLOGY. 1993;62(2):352-361.
GIEFFERS, C., ENGELHARDT, W., BRENZEL, G., MATSUISHI, T., & Frey, J. (1993). RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT. EUROPEAN JOURNAL OF CELL BIOLOGY, 62(2), 352-361.
GIEFFERS, C, ENGELHARDT, W, BRENZEL, G, MATSUISHI, T, and Frey, Jürgen. 1993. “RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT”. EUROPEAN JOURNAL OF CELL BIOLOGY 62 (2): 352-361.
GIEFFERS, C., ENGELHARDT, W., BRENZEL, G., MATSUISHI, T., and Frey, J. (1993). RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT. EUROPEAN JOURNAL OF CELL BIOLOGY 62, 352-361.
GIEFFERS, C., et al., 1993. RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT. EUROPEAN JOURNAL OF CELL BIOLOGY, 62(2), p 352-361.
C. GIEFFERS, et al., “RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT”, EUROPEAN JOURNAL OF CELL BIOLOGY, vol. 62, 1993, pp. 352-361.
GIEFFERS, C., ENGELHARDT, W., BRENZEL, G., MATSUISHI, T., Frey, J.: RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT. EUROPEAN JOURNAL OF CELL BIOLOGY. 62, 352-361 (1993).
GIEFFERS, C, ENGELHARDT, W, BRENZEL, G, MATSUISHI, T, and Frey, Jürgen. “RECEPTOR-BINDING OF OSTEOBLAST-SPECIFIC FACTOR-1 (OSF-1/HB-GAM) TO HUMAN OSTEOSARCOMA CELLS PROMOTES CELL ATTACHMENT”. EUROPEAN JOURNAL OF CELL BIOLOGY 62.2 (1993): 352-361.

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