CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE

KORIOTH F, GIEFFERS C, Frey J (1994)
GENE 150(2): 395-399.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
KORIOTH, F; GIEFFERS, C; Frey, JürgenUniBi
Einrichtung
Fakultät für Chemie > Biochemie II
Abstract / Bemerkung
Sequence information for aspartyl beta-hydroxylase (AspH), which specifically hydroxylates one Asp or Asn residue in certain epidermal growth factor (EGF)-like domains oi a number of proteins, is so far only described for bovine species. We have isolated a 4.3-kb cDNA encoding the human AspH (hAspH) by immunoscreening of a human osteosarcoma (MG63) cDNA library in lambda ZAP with an antiserum raised against membrane fractions of these cells. Northern blot analyses revealed two transcripts with lengths of 2.6 and 4.3 kb. The deduced amino acid (aa) sequence of this cDNA encodes a protein of 757 aa (85 kDa). Comparison with the deduced bovine AspH (bAspH) aa sequence showed striking differences in the N-terminal portion of this protein. In vitro transcription and translation in the presence of canine pancreas microsomes yielded a 56-kDa protein. Western blot analyses of membrane fractions from MG63 cells with AspH-specific antibodies revealed a protein of the same M(r). These results suggest a posttranslational cleavage of the catalytic C terminus in the lumen of the endoplasmic reticulum.
Stichworte
IMMUNOSCREENING; SEQUENCE COMPARISON; IN VITRO; TRANSCRIPTION-TRANSLATION; CDNA
Erscheinungsjahr
1994
Zeitschriftentitel
GENE
Band
150
Ausgabe
2
Seite(n)
395-399
ISSN
0378-1119
Page URI
https://pub.uni-bielefeld.de/record/1641738

Zitieren

KORIOTH F, GIEFFERS C, Frey J. CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE. 1994;150(2):395-399.
KORIOTH, F., GIEFFERS, C., & Frey, J. (1994). CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE, 150(2), 395-399. https://doi.org/10.1016/0378-1119(94)90460-X
KORIOTH, F, GIEFFERS, C, and Frey, Jürgen. 1994. “CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE”. GENE 150 (2): 395-399.
KORIOTH, F., GIEFFERS, C., and Frey, J. (1994). CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE 150, 395-399.
KORIOTH, F., GIEFFERS, C., & Frey, J., 1994. CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE, 150(2), p 395-399.
F. KORIOTH, C. GIEFFERS, and J. Frey, “CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE”, GENE, vol. 150, 1994, pp. 395-399.
KORIOTH, F., GIEFFERS, C., Frey, J.: CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE. 150, 395-399 (1994).
KORIOTH, F, GIEFFERS, C, and Frey, Jürgen. “CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE”. GENE 150.2 (1994): 395-399.

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