EXTENT OF SHOCK-INDUCED MEMBRANE LEAKAGE IN HUMAN AND MOUSE MYOTUBES DEPENDS ON DYSTROPHIN

MENKE A, Jockusch H (1995)
JOURNAL OF CELL SCIENCE 108: 727-733.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
A lack of the cytoskeletal protein dystrophin causes muscle fiber necrosis in Duchenne/Becker muscular dystrophies (DMD/BMD) and in murine X-linked muscular dystrophy (MDX). However, no overt disease symptoms are observed in dystrophin-less cultured myotubes, and the biological function of dystrophin in normal muscle cells is still unknown. In this work, we have extended our studies on a model system, using hypoosmotic shock to determine stress resistance of muscle cells. In frozen sections of control human and mouse myotubes, dystrophin was shown to be localized at the cell periphery as in mature muscle fibers. Dystrophin-less DMD and MDX myotubes were more susceptible to hypoosmotic shock than controls, as monitored by the uptake of external horseradish peroxidase and release of the soluble enzymes creatine kinase or pyruvate kinase and of radiolabelled proteins. Control experiments indicated that this difference is not due to differences in metabolism or ion fluxes. Treatment with cytochalasin D drastically increased the shock sensitivity of myotubes and abolished the difference between dystrophin-less and control cells. These results lend further support to the suggested stabilizing role of dystrophin in the context of the membrane-cytoskeletal complex.
Stichworte
CYTOCHALASIN D; OSMOTIC SHOCK; MICROFILAMENT SYSTEM; DYSTROPHIN; DUCHENNE DYSTROPHY; MDX MOUSE; MYOTUBE; MUSCLE CULTURE; SARCOLEMMA
Erscheinungsjahr
1995
Zeitschriftentitel
JOURNAL OF CELL SCIENCE
Band
108
Seite(n)
727-733
ISSN
0021-9533
Page URI
https://pub.uni-bielefeld.de/record/1641463

Zitieren

MENKE A, Jockusch H. EXTENT OF SHOCK-INDUCED MEMBRANE LEAKAGE IN HUMAN AND MOUSE MYOTUBES DEPENDS ON DYSTROPHIN. JOURNAL OF CELL SCIENCE. 1995;108:727-733.
MENKE, A., & Jockusch, H. (1995). EXTENT OF SHOCK-INDUCED MEMBRANE LEAKAGE IN HUMAN AND MOUSE MYOTUBES DEPENDS ON DYSTROPHIN. JOURNAL OF CELL SCIENCE, 108, 727-733.
MENKE, A., and Jockusch, H. (1995). EXTENT OF SHOCK-INDUCED MEMBRANE LEAKAGE IN HUMAN AND MOUSE MYOTUBES DEPENDS ON DYSTROPHIN. JOURNAL OF CELL SCIENCE 108, 727-733.
MENKE, A., & Jockusch, H., 1995. EXTENT OF SHOCK-INDUCED MEMBRANE LEAKAGE IN HUMAN AND MOUSE MYOTUBES DEPENDS ON DYSTROPHIN. JOURNAL OF CELL SCIENCE, 108, p 727-733.
A. MENKE and H. Jockusch, “EXTENT OF SHOCK-INDUCED MEMBRANE LEAKAGE IN HUMAN AND MOUSE MYOTUBES DEPENDS ON DYSTROPHIN”, JOURNAL OF CELL SCIENCE, vol. 108, 1995, pp. 727-733.
MENKE, A., Jockusch, H.: EXTENT OF SHOCK-INDUCED MEMBRANE LEAKAGE IN HUMAN AND MOUSE MYOTUBES DEPENDS ON DYSTROPHIN. JOURNAL OF CELL SCIENCE. 108, 727-733 (1995).
MENKE, A, and Jockusch, Harald. “EXTENT OF SHOCK-INDUCED MEMBRANE LEAKAGE IN HUMAN AND MOUSE MYOTUBES DEPENDS ON DYSTROPHIN”. JOURNAL OF CELL SCIENCE 108 (1995): 727-733.

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