A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS

Selbitschka W, DRESING U, HAGEN M, NIEMANN S, Pühler A (1995)
FEMS MICROBIOLOGY ECOLOGY 16(3): 223-232.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
DOI
Autor*in
Selbitschka, WernerUniBi; DRESING, U; HAGEN, M; NIEMANN, S; Pühler, AlfredUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
Using a newly developed integration vector, the Escherichia coli gusA gene conferring GUS-activity or the firefly (Photinus pyralis) he gene mediating bioluminescence were integrated into a non-essential site of the chromosome of Rhizobium meliloti 2011. The integration of the constitutively expressed marker genes into the chosen site per se did not affect the strains' ability to perform homologous recombination, its growth characteristics or its symbiotic nitrogen fixation. Comparative microcosm analyses between the bioluminescent, recombination-proficient (RecA(+)) R. meliloti strain L33 whose construction is reported in this paper, and its previously described recombination-deficient (RecA(-)) isogenic counterpart L1 indicate that RecA(-) strains of Rhizobium are safe hosts for deliberate release experiments.
Stichworte
INTEGRATION VECTOR; FIREFLY LUCIFERASE; BETA-GLUCURONIDASE; COMPETITION; BIOLOGICAL CONTAINMENT; RHIZOBIUM MELILOTI
Erscheinungsjahr
1995
Zeitschriftentitel
FEMS MICROBIOLOGY ECOLOGY
Band
16
Ausgabe
3
Seite(n)
223-232
ISSN
0168-6496
Page URI
https://pub.uni-bielefeld.de/record/1641351

Zitieren

Selbitschka W, DRESING U, HAGEN M, NIEMANN S, Pühler A. A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS. FEMS MICROBIOLOGY ECOLOGY. 1995;16(3):223-232.
Selbitschka, W., DRESING, U., HAGEN, M., NIEMANN, S., & Pühler, A. (1995). A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS. FEMS MICROBIOLOGY ECOLOGY, 16(3), 223-232. https://doi.org/10.1111/j.1574-6941.1995.tb00286.x
Selbitschka, Werner, DRESING, U, HAGEN, M, NIEMANN, S, and Pühler, Alfred. 1995. “A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS”. FEMS MICROBIOLOGY ECOLOGY 16 (3): 223-232.
Selbitschka, W., DRESING, U., HAGEN, M., NIEMANN, S., and Pühler, A. (1995). A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS. FEMS MICROBIOLOGY ECOLOGY 16, 223-232.
Selbitschka, W., et al., 1995. A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS. FEMS MICROBIOLOGY ECOLOGY, 16(3), p 223-232.
W. Selbitschka, et al., “A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS”, FEMS MICROBIOLOGY ECOLOGY, vol. 16, 1995, pp. 223-232.
Selbitschka, W., DRESING, U., HAGEN, M., NIEMANN, S., Pühler, A.: A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS. FEMS MICROBIOLOGY ECOLOGY. 16, 223-232 (1995).
Selbitschka, Werner, DRESING, U, HAGEN, M, NIEMANN, S, and Pühler, Alfred. “A BIOLOGICAL CONTAINMENT SYSTEM FOR RHIZOBIUM-MELILOTI BASED ON THE USE OF RECOMBINATION-DEFICIENT (RECA(-)) STRAINS”. FEMS MICROBIOLOGY ECOLOGY 16.3 (1995): 223-232.
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Suchen in

Google Scholar