THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT
HOFER B, RUGE M, Dreiseikelmann B (1995)
JOURNAL OF BACTERIOLOGY 177(11): 3080-3086.
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Autor*in
HOFER, B;
RUGE, M;
Dreiseikelmann, BrigitteUniBi
Abstract / Bemerkung
Previous work has shown that the sieA gene of Salmonella bacteriophage P22 is located between the genes mnt and 16. We cloned DSA fragments of the region into multicopy vectors and tested the transformants for mediating superinfection exclusion. Subcloning, phenotypical tests, and DNA sequencing resulted in the identification of the sieA gene. There are two possible initiation codons within one open reading frame of 492 or 480 bp. The deduced amino acid sequence leads to a hypothetical polypeptide with a calculated molecular mass of 18.8 or 18.3 kDa, respectively. According to three hydrophobic regions, all of which are long enough to span the membrane, the product of sieA should be a protein of the inner membrane of a P22-lysogenic cell of Salmonella typhimurium. The SieA protein vt as moderately overproduced from an expression vector in cultures of Escherichia coil and could be recovered from the membrane fraction.
Erscheinungsjahr
1995
Zeitschriftentitel
JOURNAL OF BACTERIOLOGY
Band
177
Ausgabe
11
Seite(n)
3080-3086
ISSN
0021-9193
Page URI
https://pub.uni-bielefeld.de/record/1640825
Zitieren
HOFER B, RUGE M, Dreiseikelmann B. THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT. JOURNAL OF BACTERIOLOGY. 1995;177(11):3080-3086.
HOFER, B., RUGE, M., & Dreiseikelmann, B. (1995). THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT. JOURNAL OF BACTERIOLOGY, 177(11), 3080-3086. https://doi.org/10.1128/jb.177.11.3080-3086.1995
HOFER, B, RUGE, M, and Dreiseikelmann, Brigitte. 1995. “THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT”. JOURNAL OF BACTERIOLOGY 177 (11): 3080-3086.
HOFER, B., RUGE, M., and Dreiseikelmann, B. (1995). THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT. JOURNAL OF BACTERIOLOGY 177, 3080-3086.
HOFER, B., RUGE, M., & Dreiseikelmann, B., 1995. THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT. JOURNAL OF BACTERIOLOGY, 177(11), p 3080-3086.
B. HOFER, M. RUGE, and B. Dreiseikelmann, “THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT”, JOURNAL OF BACTERIOLOGY, vol. 177, 1995, pp. 3080-3086.
HOFER, B., RUGE, M., Dreiseikelmann, B.: THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT. JOURNAL OF BACTERIOLOGY. 177, 3080-3086 (1995).
HOFER, B, RUGE, M, and Dreiseikelmann, Brigitte. “THE SUPERINFECTION EXCLUSION GENE (SIEA) OF BACTERIOPHAGE-P22 - IDENTIFICATION AND OVEREXPRESSION OF THE GENE AND LOCALIZATION OF THE GENE-PRODUCT”. JOURNAL OF BACTERIOLOGY 177.11 (1995): 3080-3086.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
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UNIPROT
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Superinfection exclusion protein A (UNIPROT: Q38674)
Organism: Enterobacteria phage P22
Download in FASTA format
Organism: Enterobacteria phage P22
Download in FASTA format
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