EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING

GONSIOR S, Hinssen H (1995)
CELL MOTILITY AND THE CYTOSKELETON 31(3): 196-206.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
We have investigated the binding of gelsolin to thin myofilaments in situ and their stability against severing. Differentiated myotubes from chicken skeletal muscle containing cross-striated myofibrils were permeabilized with Triton X-100 and incubated with gelsolin. Immunofluorescence microscopy localized both endogenous and exogenous gelsolin in the I-Z-I-regions of the sarcomers. The staining pattern suggested a binding of the exogenous gelsolin along the entire length of the thin filaments. This binding was Ca2+ dependent, but gelsolin was not removed after subsequent addition of EGTA. The fluorescence staining for actin remained unchanged after gelsolin incubation, indicating that thin filaments in cross-striated myofibrils were resistant to the severing action of gelsolin, in contrast to the microfilaments in stress fibers. After extraction of the permeabilized cells with high ionic strength to remove tropomyosin and myosin, gelsolin still bound along the entire thin filament and the actin pattern also remained unchanged. After Triton X-100 permeabilization and high ionic strength extraction, the giant protein nebulin was found to be still present as a myofibrillar component. Gelsolin treatment after high salt extraction affected neither actin nor nebulin in the thin filaments. We therefore conclude that nebulin confers the gelsolin resistance to the sarcomeric actin filaments. (C) 1995 Wiley-Liss, Inc.
Stichworte
GELSOLIN; MYOFIBRILS; IMMUNOFLUORESCENCE; ACTIN; NEBULIN
Erscheinungsjahr
1995
Zeitschriftentitel
CELL MOTILITY AND THE CYTOSKELETON
Band
31
Ausgabe
3
Seite(n)
196-206
ISSN
0886-1544
eISSN
1097-0169
Page URI
https://pub.uni-bielefeld.de/record/1640522

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GONSIOR S, Hinssen H. EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON. 1995;31(3):196-206.
GONSIOR, S., & Hinssen, H. (1995). EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON, 31(3), 196-206. doi:10.1002/cm.970310303
GONSIOR, S., and Hinssen, H. (1995). EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON 31, 196-206.
GONSIOR, S., & Hinssen, H., 1995. EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON, 31(3), p 196-206.
S. GONSIOR and H. Hinssen, “EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING”, CELL MOTILITY AND THE CYTOSKELETON, vol. 31, 1995, pp. 196-206.
GONSIOR, S., Hinssen, H.: EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON. 31, 196-206 (1995).
GONSIOR, S, and Hinssen, Horst. “EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING”. CELL MOTILITY AND THE CYTOSKELETON 31.3 (1995): 196-206.

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