Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation

Bardouille C, Vullhorst D, Jockusch H (1996)
FEBS letters 396(2-3): 177-180.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor/in
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Abstract / Bemerkung
The chloride channel ClC-1 is required to maintain a normal excitability of mature muscle fibers; its blockade leads to hyperexcitability, the hallmark of the disease myotonia. In mouse and rat myotubes, representing the embryonic stage of muscle, ClC-1 mRNA is not detectable by Northern blotting. From neonatal to adult, ClC-1 expression increases at least fourfold. Using RT-PCR and hybridization on cultured myotubes we found ClC-1 mRNA at a level of 0.4-1.1% of that in mature mouse muscle, and less than or equal to 0.01% in myoblasts, at stages when desmin mRNA levels are already high. The level of ClC-1 mRNA is thus a sensitive and specific indicator of the maturation of skeletal muscle cells.
Stichworte
quantitative RT-PCR; skeletal muscle development; cell culture; chloride channel 1; myotonia
Erscheinungsjahr
1996
Zeitschriftentitel
FEBS letters
Band
396
Ausgabe
2-3
Seite(n)
177-180
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1638132

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Bardouille C, Vullhorst D, Jockusch H. Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters. 1996;396(2-3):177-180.
Bardouille, C., Vullhorst, D., & Jockusch, H. (1996). Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters, 396(2-3), 177-180. doi:10.1016/0014-5793(96)01098-8
Bardouille, C., Vullhorst, D., and Jockusch, H. (1996). Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters 396, 177-180.
Bardouille, C., Vullhorst, D., & Jockusch, H., 1996. Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters, 396(2-3), p 177-180.
C. Bardouille, D. Vullhorst, and H. Jockusch, “Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation”, FEBS letters, vol. 396, 1996, pp. 177-180.
Bardouille, C., Vullhorst, D., Jockusch, H.: Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters. 396, 177-180 (1996).
Bardouille, C, Vullhorst, D, and Jockusch, Harald. “Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation”. FEBS letters 396.2-3 (1996): 177-180.

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