Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation

Bardouille C, Vullhorst D, Jockusch H (1996)
FEBS letters 396(2-3): 177-180.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Bardouille, C; Vullhorst, D; Jockusch, HaraldUniBi
Einrichtung
Fakultät für Biologie
Abstract / Bemerkung
The chloride channel ClC-1 is required to maintain a normal excitability of mature muscle fibers; its blockade leads to hyperexcitability, the hallmark of the disease myotonia. In mouse and rat myotubes, representing the embryonic stage of muscle, ClC-1 mRNA is not detectable by Northern blotting. From neonatal to adult, ClC-1 expression increases at least fourfold. Using RT-PCR and hybridization on cultured myotubes we found ClC-1 mRNA at a level of 0.4-1.1% of that in mature mouse muscle, and less than or equal to 0.01% in myoblasts, at stages when desmin mRNA levels are already high. The level of ClC-1 mRNA is thus a sensitive and specific indicator of the maturation of skeletal muscle cells.
Stichworte
quantitative RT-PCR; skeletal muscle development; cell culture; chloride channel 1; myotonia
Erscheinungsjahr
1996
Zeitschriftentitel
FEBS letters
Band
396
Ausgabe
2-3
Seite(n)
177-180
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1638132

Zitieren

Bardouille C, Vullhorst D, Jockusch H. Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters. 1996;396(2-3):177-180.
Bardouille, C., Vullhorst, D., & Jockusch, H. (1996). Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters, 396(2-3), 177-180. https://doi.org/10.1016/0014-5793(96)01098-8
Bardouille, C, Vullhorst, D, and Jockusch, Harald. 1996. “Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation”. FEBS letters 396 (2-3): 177-180.
Bardouille, C., Vullhorst, D., and Jockusch, H. (1996). Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters 396, 177-180.
Bardouille, C., Vullhorst, D., & Jockusch, H., 1996. Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters, 396(2-3), p 177-180.
C. Bardouille, D. Vullhorst, and H. Jockusch, “Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation”, FEBS letters, vol. 396, 1996, pp. 177-180.
Bardouille, C., Vullhorst, D., Jockusch, H.: Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation. FEBS letters. 396, 177-180 (1996).
Bardouille, C, Vullhorst, D, and Jockusch, Harald. “Expression of chloride channel 1 mRNA in cultured myogenic cells: A marker of myotube maturation”. FEBS letters 396.2-3 (1996): 177-180.

10 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

CLC Chloride Channels and Transporters: Structure, Function, Physiology, and Disease.
Jentsch TJ, Pusch M., Physiol Rev 98(3), 2018
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HTS-Compatible Patient-Derived Cell-Based Assay to Identify Small Molecule Modulators of Aberrant Splicing in Myotonic Dystrophy Type 1.
O'Leary DA, Vargas L, Sharif O, Garcia ME, Sigal YJ, Chow SK, Schmedt C, Caldwell JS, Brinker A, Engels IH., Curr Chem Genomics 4(), 2010
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