The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2

Lichte A, Kolkenbrock H, Tschesche H (1996)
FEBS LETTERS 397(2-3): 277-282.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
A truncated form of the membrane-type matrix metalloproteinase-1 [(Ala(21)-Ile(318))proMT1-MMP] lacking the hemopexin-like and trans-membrane domain was produced in E. coli. We demonstrate that the recombinant proenzyme was autoproteolytically processed to a fully active catalytic domain with N-terminal Ile(114). The catalytic domain of MT1-MMP initiated the activation of progelatinase A and progelatinase A complexed with tissue inhibitor of metalloproteinases-2 (TIMP-2). As a typical soluble metalloproteinase it was able to cleave physiologic as well as synthetic substrates. Our kinetic data demonstrate that TIMP-2 is a potent inhibitor for the recombinant enzyme.
Stichworte
membrane-type matrix metalloproteinase; matrix metalloproteinase; gelatinase A; type IV collagenase activation; TIMP-2
Erscheinungsjahr
1996
Zeitschriftentitel
FEBS LETTERS
Band
397
Ausgabe
2-3
Seite(n)
277-282
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1638047

Zitieren

Lichte A, Kolkenbrock H, Tschesche H. The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2. FEBS LETTERS. 1996;397(2-3):277-282.
Lichte, A., Kolkenbrock, H., & Tschesche, H. (1996). The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2. FEBS LETTERS, 397(2-3), 277-282. doi:10.1016/S0014-5793(96)01206-9
Lichte, A., Kolkenbrock, H., and Tschesche, H. (1996). The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2. FEBS LETTERS 397, 277-282.
Lichte, A., Kolkenbrock, H., & Tschesche, H., 1996. The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2. FEBS LETTERS, 397(2-3), p 277-282.
A. Lichte, H. Kolkenbrock, and H. Tschesche, “The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2”, FEBS LETTERS, vol. 397, 1996, pp. 277-282.
Lichte, A., Kolkenbrock, H., Tschesche, H.: The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2. FEBS LETTERS. 397, 277-282 (1996).
Lichte, A, Kolkenbrock, H, and Tschesche, Harald. “The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2”. FEBS LETTERS 397.2-3 (1996): 277-282.

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PMID: 8955363
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