Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase

Stracke R, De Vos RCH, Bartelniewoehner L, Ishihara H, Sagasser M, Martens S, Weisshaar B (2009)
Planta 229(2): 427-445.

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DOI
Autor*in
Stracke, RalfUniBi ; De Vos, Ric C. H.; Bartelniewoehner, Lutz; Ishihara, Hirofumi; Sagasser, MartinUniBi; Martens, Stefan; Weisshaar, BerndUniBi
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe B. Weisshaar
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Fakultät für Biologie > Genetik und Genomik der Pflanzen
Abstract / Bemerkung
Flavonol synthase (FLS) (EC-number 1.14.11.23), the enzyme that catalyses the conversion of flavonols into dihydroflavonols, is part of the flavonoid biosynthesis pathway. In Arabidopsis thaliana, this activity is thought to be encoded by several loci. In addition to the FLAVONOL SYNTHASE1 (FLS1) locus that has been confirmed by enzyme activity assays, loci displaying similarity of the deduced amino acid sequences to FLS1 have been identified. We studied the putative A. thaliana FLS gene family using a combination of genetic and metabolite analysis approaches. Although several of the FLS gene family members are expressed, only FLS1 appeared to influence flavonoid biosynthesis. Seedlings of an A. thaliana fls1 null mutant (fls1-2) show enhanced anthocyanin levels, drastic reduction in flavonol glycoside content and concomitant accumulation of glycosylated forms of dihydroflavonols, the substrate of the FLS reaction. By using a leucoanthocyanidin dioxygenase (ldox) fls1-2 double mutant, we present evidence that the remaining flavonol glycosides found in the fls1-2 mutant are synthesized in planta by the FLS-like side activity of the LDOX enzyme.
Stichworte
Flavonol; Arabidopsis; Dihydroflavonol; Flavonoid biosynthesis; synthase; Leucoanthocyanidin dioxygenase
Erscheinungsjahr
2009
Zeitschriftentitel
Planta
Band
229
Ausgabe
2
Seite(n)
427-445
ISSN
0032-0935
eISSN
1432-2048
Page URI
https://pub.uni-bielefeld.de/record/1636402

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Stracke R, De Vos RCH, Bartelniewoehner L, et al. Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta. 2009;229(2):427-445.
Stracke, R., De Vos, R. C. H., Bartelniewoehner, L., Ishihara, H., Sagasser, M., Martens, S., & Weisshaar, B. (2009). Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta, 229(2), 427-445. https://doi.org/10.1007/s00425-008-0841-y
Stracke, Ralf, De Vos, Ric C. H., Bartelniewoehner, Lutz, Ishihara, Hirofumi, Sagasser, Martin, Martens, Stefan, and Weisshaar, Bernd. 2009. “Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase”. Planta 229 (2): 427-445.
Stracke, R., De Vos, R. C. H., Bartelniewoehner, L., Ishihara, H., Sagasser, M., Martens, S., and Weisshaar, B. (2009). Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta 229, 427-445.
Stracke, R., et al., 2009. Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta, 229(2), p 427-445.
R. Stracke, et al., “Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase”, Planta, vol. 229, 2009, pp. 427-445.
Stracke, R., De Vos, R.C.H., Bartelniewoehner, L., Ishihara, H., Sagasser, M., Martens, S., Weisshaar, B.: Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta. 229, 427-445 (2009).
Stracke, Ralf, De Vos, Ric C. H., Bartelniewoehner, Lutz, Ishihara, Hirofumi, Sagasser, Martin, Martens, Stefan, and Weisshaar, Bernd. “Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase”. Planta 229.2 (2009): 427-445.

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