Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis
Borelli S, Monn A, Meyer J, Berger U, Honegger HP, Lautenschlager S (2009)
INFECTION 37(1): 26-28.
Zeitschriftenaufsatz
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Autor*in
Borelli, S.;
Monn, A.;
Meyer, J.;
Berger, UrsulaUniBi;
Honegger, H. P.;
Lautenschlager, St.
Einrichtung
Abstract / Bemerkung
Recent trends in Western Europe show an increase in sexually transmitted infections. Surveillance data in Switzerland confirm this rising trend. Notifications of syphilis cases nearly doubled in the year 2002 and almost tripled in 2003. This trend necessitates an early correct diagnosis making reliable screening tests mandatory. In the presented study a particle gel immunoassay (ID-PaGIA syphilis antibody test, Diamed) using recombinant treponemal antigens TpN15, TpN17 and TpN47 was evaluated as a screening test in comparison to the currently used Treponema pallidum particle agglutination test (Serodia-TPPA, Fujirebio). Serum samples were obtained from a cross-sectional sero-epidemiological study among men who have sex with men. Samples were tested with the PaGia and the TPPA. In the case of equivocal results a titrated TPPA of an external laboratory was used as a confirmation test. In total 650 serum samples (48 seropositive patients, 602 negative) were evaluated. The PaGIA showed a sensitivity of 0.89 (43/48) and the TPPA of 0.83 (40/48). This difference was not statistically relevant (p = 0.4). The particle gel assay showed a significantly higher specificity (1.0) compared to the TPPA (0.98) (p = 0.004). The PaGIA showed a sensitivity comparable to that of other treponemal tests with an even better specificity. Advantages of the PaGIA are the fast reaction time of only 20 min and the simplicity of the procedure with minimal technical equipment.
Erscheinungsjahr
2009
Zeitschriftentitel
INFECTION
Band
37
Ausgabe
1
Seite(n)
26-28
ISSN
0300-8126
eISSN
1439-0973
Page URI
https://pub.uni-bielefeld.de/record/1635263
Zitieren
Borelli S, Monn A, Meyer J, Berger U, Honegger HP, Lautenschlager S. Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis. INFECTION. 2009;37(1):26-28.
Borelli, S., Monn, A., Meyer, J., Berger, U., Honegger, H. P., & Lautenschlager, S. (2009). Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis. INFECTION, 37(1), 26-28. https://doi.org/10.1007/s15010-008-7082-7
Borelli, S., Monn, A., Meyer, J., Berger, Ursula, Honegger, H. P., and Lautenschlager, St. 2009. “Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis”. INFECTION 37 (1): 26-28.
Borelli, S., Monn, A., Meyer, J., Berger, U., Honegger, H. P., and Lautenschlager, S. (2009). Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis. INFECTION 37, 26-28.
Borelli, S., et al., 2009. Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis. INFECTION, 37(1), p 26-28.
S. Borelli, et al., “Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis”, INFECTION, vol. 37, 2009, pp. 26-28.
Borelli, S., Monn, A., Meyer, J., Berger, U., Honegger, H.P., Lautenschlager, S.: Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis. INFECTION. 37, 26-28 (2009).
Borelli, S., Monn, A., Meyer, J., Berger, Ursula, Honegger, H. P., and Lautenschlager, St. “Evaluation of a Particle Gel Immunoassay as a Screening Test for Syphilis”. INFECTION 37.1 (2009): 26-28.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
2 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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PMID: 29158917
Njelekela M, Mpembeni R, Muhihi A, Ulenga N, Aris E, Kakoko D., AIDS Res Treat 2017(), 2017
PMID: 29158917
Performance evaluation of the Elecsys syphilis assay for the detection of total antibodies to Treponema pallidum.
Enders M, Hunjet A, Gleich M, Imdahl R, Mühlbacher A, Schennach H, Chaiwong K, Sakuldamrongpanich T, Turhan A, Sertöz R, Wolf E, Mayer W, Tao C, Wang LL, Semprini S, Sambri V., Clin Vaccine Immunol 22(1), 2015
PMID: 25355799
Enders M, Hunjet A, Gleich M, Imdahl R, Mühlbacher A, Schennach H, Chaiwong K, Sakuldamrongpanich T, Turhan A, Sertöz R, Wolf E, Mayer W, Tao C, Wang LL, Semprini S, Sambri V., Clin Vaccine Immunol 22(1), 2015
PMID: 25355799
17 References
Daten bereitgestellt von Europe PubMed Central.
Recombinant antigen-based enzyme immunoassay for screening of Treponema pallidum antibodies in blood bank routine.
Zrein M, Maure I, Boursier F, Soufflet L., J. Clin. Microbiol. 33(3), 1995
PMID: 7751351
Zrein M, Maure I, Boursier F, Soufflet L., J. Clin. Microbiol. 33(3), 1995
PMID: 7751351
Guidelines for serological testing for syphilis.
Young H., Sex Transm Infect 76(5), 2000
PMID: 11141863
Young H., Sex Transm Infect 76(5), 2000
PMID: 11141863
Syphilis fast latex agglutination test, a rapid confirmatory test.
Fears MB, Pope V., Clin. Diagn. Lab. Immunol. 8(4), 2001
PMID: 11427439
Fears MB, Pope V., Clin. Diagn. Lab. Immunol. 8(4), 2001
PMID: 11427439
Evaluation of recomWell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis.
Sambri V, Marangoni A, Simone MA, D'Antuono A, Negosanti M, Cevenini R., Clin. Microbiol. Infect. 7(4), 2001
PMID: 11422242
Sambri V, Marangoni A, Simone MA, D'Antuono A, Negosanti M, Cevenini R., Clin. Microbiol. Infect. 7(4), 2001
PMID: 11422242
Evaluation of the serodia Treponema pallidum particle agglutination, the Murex Syphilis ICE and the Enzywell TP tests for serodiagnosis of syphilis.
Aktas G, Young H, Moyes A, Badur S., Int J STD AIDS 16(4), 2005
PMID: 15899082
Aktas G, Young H, Moyes A, Badur S., Int J STD AIDS 16(4), 2005
PMID: 15899082
Evaluation of INNO-LIA syphilis assay as a confirmatory test for syphilis.
Hagedorn HJ, Kraminer-Hagedorn A, De Bosschere K, Hulstaert F, Pottel H, Zrein M., J. Clin. Microbiol. 40(3), 2002
PMID: 11880425
Hagedorn HJ, Kraminer-Hagedorn A, De Bosschere K, Hulstaert F, Pottel H, Zrein M., J. Clin. Microbiol. 40(3), 2002
PMID: 11880425
Comparative evaluation of nine different enzyme-linked immunosorbent assays for determination of antibodies against Treponema pallidum in patients with primary syphilis.
Schmidt BL, Edjlalipour M, Luger A., J. Clin. Microbiol. 38(3), 2000
PMID: 10699042
Schmidt BL, Edjlalipour M, Luger A., J. Clin. Microbiol. 38(3), 2000
PMID: 10699042
Biological false-positive tests comprise a high proportion of Venereal Disease Research Laboratory reactions in an analysis of 300,000 sera.
Geusau A, Kittler H, Hein U, Dangl-Erlach E, Stingl G, Tschachler E., Int J STD AIDS 16(11), 2005
PMID: 16303064
Geusau A, Kittler H, Hein U, Dangl-Erlach E, Stingl G, Tschachler E., Int J STD AIDS 16(11), 2005
PMID: 16303064
Novel recombinant-antigen enzyme immunoassay for serological diagnosis of syphilis.
Young H, Moyes A, Seagar L, McMillan A., J. Clin. Microbiol. 36(4), 1998
PMID: 9542908
Young H, Moyes A, Seagar L, McMillan A., J. Clin. Microbiol. 36(4), 1998
PMID: 9542908
Evaluation of LIAISON Treponema Screen, a novel recombinant antigen-based chemiluminescence immunoassay for laboratory diagnosis of syphilis.
Marangoni A, Sambri V, Accardo S, Cavrini F, D'Antuono A, Moroni A, Storni E, Cevenini R., Clin. Diagn. Lab. Immunol. 12(10), 2005
PMID: 16210488
Marangoni A, Sambri V, Accardo S, Cavrini F, D'Antuono A, Moroni A, Storni E, Cevenini R., Clin. Diagn. Lab. Immunol. 12(10), 2005
PMID: 16210488
Validation of the INNO-LIA syphilis kit as a confirmatory assay for Treponema pallidum antibodies.
Ebel A, Vanneste L, Cardinaels M, Sablon E, Samson I, De Bosschere K, Hulstaert F, Zrein M., J. Clin. Microbiol. 38(1), 2000
PMID: 10618090
Ebel A, Vanneste L, Cardinaels M, Sablon E, Samson I, De Bosschere K, Hulstaert F, Zrein M., J. Clin. Microbiol. 38(1), 2000
PMID: 10618090
Sexually transmitted infections in Switzerland: return of the classics.
Lautenschlager S., Dermatology (Basel) 210(2), 2005
PMID: 15724096
Lautenschlager S., Dermatology (Basel) 210(2), 2005
PMID: 15724096
A new recombinant antigen latex agglutination test (Syphilis Fast) for the rapid serological diagnosis of syphilis.
Young H, Moyes A, de Ste Croix I, McMillan A., Int J STD AIDS 9(4), 1998
PMID: 9598745
Young H, Moyes A, de Ste Croix I, McMillan A., Int J STD AIDS 9(4), 1998
PMID: 9598745
Evaluation of a new particle gel immunoassay for determination of antibodies against Treponema pallidum.
Schmidt BL., J. Clin. Microbiol. 42(6), 2004
PMID: 15184485
Schmidt BL., J. Clin. Microbiol. 42(6), 2004
PMID: 15184485
Diagnosis of syphilis: clinical and laboratory problems.
Lautenschlager S., J Dtsch Dermatol Ges 4(12), 2006
PMID: 17176415
Lautenschlager S., J Dtsch Dermatol Ges 4(12), 2006
PMID: 17176415
Recombinant Treponema pallidum antigens in syphilis serology.
Gerber A, Krell S, Morenz J., Immunobiology 196(5), 1996
PMID: 9145331
Gerber A, Krell S, Morenz J., Immunobiology 196(5), 1996
PMID: 9145331
Increased incidence of sexually transmitted infections in Geneva, Switzerland.
Abraham S, Toutous-Trellu L, Pechere M, Hugonnet S, Liassine N, Yerly S, Rohner P, Ninet B, Hirschel B, Piguet V., Dermatology (Basel) 212(1), 2006
PMID: 16319473
Abraham S, Toutous-Trellu L, Pechere M, Hugonnet S, Liassine N, Yerly S, Rohner P, Ninet B, Hirschel B, Piguet V., Dermatology (Basel) 212(1), 2006
PMID: 16319473
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