Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome

Unell M, Abraham PE, Shah M, Zhang B, Rückert C, VerBerkmoes NC, Jansson JK (2009)
JOURNAL OF PROTEOME RESEARCH 8(4): 1953-1964.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
Stichworte
shotgun proteomics; phenol; 4-chlorophenol; 4-nitrophenol; Arthrobacter chlorophenolicus; hydroxyquinol dioxygenase; 2D-LC-ES-MS/MS
Erscheinungsjahr
2009
Zeitschriftentitel
JOURNAL OF PROTEOME RESEARCH
Band
8
Ausgabe
4
Seite(n)
1953-1964
ISSN
1535-3893
eISSN
1535-3907
Page URI
https://pub.uni-bielefeld.de/record/1634429

Zitieren

Unell M, Abraham PE, Shah M, et al. Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome. JOURNAL OF PROTEOME RESEARCH. 2009;8(4):1953-1964.
Unell, M., Abraham, P. E., Shah, M., Zhang, B., Rückert, C., VerBerkmoes, N. C., & Jansson, J. K. (2009). Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome. JOURNAL OF PROTEOME RESEARCH, 8(4), 1953-1964. doi:10.1021/pr800897c
Unell, M., Abraham, P. E., Shah, M., Zhang, B., Rückert, C., VerBerkmoes, N. C., and Jansson, J. K. (2009). Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome. JOURNAL OF PROTEOME RESEARCH 8, 1953-1964.
Unell, M., et al., 2009. Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome. JOURNAL OF PROTEOME RESEARCH, 8(4), p 1953-1964.
M. Unell, et al., “Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome”, JOURNAL OF PROTEOME RESEARCH, vol. 8, 2009, pp. 1953-1964.
Unell, M., Abraham, P.E., Shah, M., Zhang, B., Rückert, C., VerBerkmoes, N.C., Jansson, J.K.: Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome. JOURNAL OF PROTEOME RESEARCH. 8, 1953-1964 (2009).
Unell, Maria, Abraham, Paul E., Shah, Manesh, Zhang, Bing, Rückert, Christian, VerBerkmoes, Nathan C., and Jansson, Janet K. “Impact of Phenolic Substrate and Growth Temperature on the Arthrobacter chlorophenolicus Proteome”. JOURNAL OF PROTEOME RESEARCH 8.4 (2009): 1953-1964.

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