Scattering suppression and confocal detection in multifocal multiphoton microscopy

Martini J, Andresen V, Anselmetti D (2007)
JOURNAL OF BIOMEDICAL OPTICS 12(3): 034010.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor/in
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Abstract / Bemerkung
We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
Stichworte
multiphoton processes; biomedical optics; stray light; confocal optics; microscopy; multiplexing
Erscheinungsjahr
2007
Zeitschriftentitel
JOURNAL OF BIOMEDICAL OPTICS
Band
12
Ausgabe
3
Art.-Nr.
034010
ISSN
1083-3668
Page URI
https://pub.uni-bielefeld.de/record/1632503

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Martini J, Andresen V, Anselmetti D. Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS. 2007;12(3): 034010.
Martini, J., Andresen, V., & Anselmetti, D. (2007). Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS, 12(3), 034010. doi:10.1117/1.2736425
Martini, J., Andresen, V., and Anselmetti, D. (2007). Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS 12:034010.
Martini, J., Andresen, V., & Anselmetti, D., 2007. Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS, 12(3): 034010.
J. Martini, V. Andresen, and D. Anselmetti, “Scattering suppression and confocal detection in multifocal multiphoton microscopy”, JOURNAL OF BIOMEDICAL OPTICS, vol. 12, 2007, : 034010.
Martini, J., Andresen, V., Anselmetti, D.: Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS. 12, : 034010 (2007).
Martini, Joerg, Andresen, Volker, and Anselmetti, Dario. “Scattering suppression and confocal detection in multifocal multiphoton microscopy”. JOURNAL OF BIOMEDICAL OPTICS 12.3 (2007): 034010.

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PMID: 17614718
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