Scattering suppression and confocal detection in multifocal multiphoton microscopy
Martini J, Andresen V, Anselmetti D (2007)
JOURNAL OF BIOMEDICAL OPTICS 12(3): 034010.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Martini, Joerg;
Andresen, Volker;
Anselmetti, DarioUniBi 
Einrichtung
Abstract / Bemerkung
We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
Stichworte
multiphoton processes;
biomedical optics;
stray light;
confocal optics;
microscopy;
multiplexing
Erscheinungsjahr
2007
Zeitschriftentitel
JOURNAL OF BIOMEDICAL OPTICS
Band
12
Ausgabe
3
Art.-Nr.
034010
ISSN
1083-3668
Page URI
https://pub.uni-bielefeld.de/record/1632503
Zitieren
Martini J, Andresen V, Anselmetti D. Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS. 2007;12(3): 034010.
Martini, J., Andresen, V., & Anselmetti, D. (2007). Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS, 12(3), 034010. https://doi.org/10.1117/1.2736425
Martini, Joerg, Andresen, Volker, and Anselmetti, Dario. 2007. “Scattering suppression and confocal detection in multifocal multiphoton microscopy”. JOURNAL OF BIOMEDICAL OPTICS 12 (3): 034010.
Martini, J., Andresen, V., and Anselmetti, D. (2007). Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS 12:034010.
Martini, J., Andresen, V., & Anselmetti, D., 2007. Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS, 12(3): 034010.
J. Martini, V. Andresen, and D. Anselmetti, “Scattering suppression and confocal detection in multifocal multiphoton microscopy”, JOURNAL OF BIOMEDICAL OPTICS, vol. 12, 2007, : 034010.
Martini, J., Andresen, V., Anselmetti, D.: Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS. 12, : 034010 (2007).
Martini, Joerg, Andresen, Volker, and Anselmetti, Dario. “Scattering suppression and confocal detection in multifocal multiphoton microscopy”. JOURNAL OF BIOMEDICAL OPTICS 12.3 (2007): 034010.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
5 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Elimination of imaging artifacts in second harmonic generation microscopy using interferometry.
Pinsard M, Schmeltz M, van der Kolk J, Patten SA, Ibrahim H, Ramunno L, Schanne-Klein MC, Légaré F., Biomed Opt Express 10(8), 2019
PMID: 31452986
Pinsard M, Schmeltz M, van der Kolk J, Patten SA, Ibrahim H, Ramunno L, Schanne-Klein MC, Légaré F., Biomed Opt Express 10(8), 2019
PMID: 31452986
Non-descanned multifocal multiphoton microscopy with a multianode photomultiplier tube.
Cha JW, Yew EY, Kim D, Subramanian J, Nedivi E, So PT., AIP Adv 5(8), 2015
PMID: 25874160
Cha JW, Yew EY, Kim D, Subramanian J, Nedivi E, So PT., AIP Adv 5(8), 2015
PMID: 25874160
Fiber-based dual-focus time-demultiplexed second harmonic generation microscopy.
Heuke S, Legesse FB, Lorenz A, Pascher T, Akimov D, Jäger M, Schmitt M, Popp J., Opt Lett 40(11), 2015
PMID: 26030543
Heuke S, Legesse FB, Lorenz A, Pascher T, Akimov D, Jäger M, Schmitt M, Popp J., Opt Lett 40(11), 2015
PMID: 26030543
Two-photon and second harmonic microscopy in clinical and translational cancer research.
Perry SW, Burke RM, Brown EB., Ann Biomed Eng 40(2), 2012
PMID: 22258888
Perry SW, Burke RM, Brown EB., Ann Biomed Eng 40(2), 2012
PMID: 22258888
Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging.
Kumar S, Dunsby C, De Beule PA, Owen DM, Anand U, Lanigan PM, Benninger RK, Davis DM, Neil MA, Anand P, Benham C, Naylor A, French PM., Opt Express 15(20), 2007
PMID: 19550524
Kumar S, Dunsby C, De Beule PA, Owen DM, Anand U, Lanigan PM, Benninger RK, Davis DM, Neil MA, Anand P, Benham C, Naylor A, French PM., Opt Express 15(20), 2007
PMID: 19550524
28 References
Daten bereitgestellt von Europe PubMed Central.
Two-photon laser scanning fluorescence microscopy.
Denk W, Strickler JH, Webb WW., Science 248(4951), 1990
PMID: 2321027
Denk W, Strickler JH, Webb WW., Science 248(4951), 1990
PMID: 2321027
Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation.
Zipfel WR, Williams RM, Christie R, Nikitin AY, Hyman BT, Webb WW., Proc. Natl. Acad. Sci. U.S.A. 100(12), 2003
PMID: 12756303
Zipfel WR, Williams RM, Christie R, Nikitin AY, Hyman BT, Webb WW., Proc. Natl. Acad. Sci. U.S.A. 100(12), 2003
PMID: 12756303
Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms.
Campagnola PJ, Loew LM., Nat. Biotechnol. 21(11), 2003
PMID: 14595363
Campagnola PJ, Loew LM., Nat. Biotechnol. 21(11), 2003
PMID: 14595363
Two-Photon Fluorescence Excitation Cross Sections of Biomolecular Probes from 690 to 960 nm.
Albota MA, Xu C, Webb WW., Appl Opt 37(31), 1998
PMID: 18301569
Albota MA, Xu C, Webb WW., Appl Opt 37(31), 1998
PMID: 18301569
AUTHOR UNKNOWN, 0
Comparison of the axial resolution of practical Nipkow-disk confocal fluorescence microscopy with that of multifocal multiphoton microscopy: theory and experiment.
Egner A, Andresen V, Hell SW., J Microsc 206(Pt 1), 2002
PMID: 12000560
Egner A, Andresen V, Hell SW., J Microsc 206(Pt 1), 2002
PMID: 12000560
Multifocal multiphoton microscopy.
Bewersdorf J, Pick R, Hell SW., Opt Lett 23(9), 1998
PMID: 18087301
Bewersdorf J, Pick R, Hell SW., Opt Lett 23(9), 1998
PMID: 18087301
Sacconi, Opt Lett 28(), 2003
High efficiency beam splitter for multifocal multiphoton microscopy.
Nielsen T, Fricke M, Hellweg D, Andresen P., J Microsc 201(Pt 3), 2001
PMID: 11240852
Nielsen T, Fricke M, Hellweg D, Andresen P., J Microsc 201(Pt 3), 2001
PMID: 11240852
Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy.
Fittinghoff D, Wiseman P, Squier J., Opt Express 7(8), 2000
PMID: 19407875
Fittinghoff D, Wiseman P, Squier J., Opt Express 7(8), 2000
PMID: 19407875
In vivo two-photon laser-scanning microscopy of Ca2+ dynamics in visual motion-sensitive neurons.
Kalb J, Nielsen T, Fricke M, Egelhaaf M, Kurtz R., Biochem. Biophys. Res. Commun. 316(2), 2004
PMID: 15020223
Kalb J, Nielsen T, Fricke M, Egelhaaf M, Kurtz R., Biochem. Biophys. Res. Commun. 316(2), 2004
PMID: 15020223
Application of multiline two-photon microscopy to functional in vivo imaging.
Kurtz R, Fricke M, Kalb J, Tinnefeld P, Sauer M., J. Neurosci. Methods 151(2), 2006
PMID: 16442636
Kurtz R, Fricke M, Kalb J, Tinnefeld P, Sauer M., J. Neurosci. Methods 151(2), 2006
PMID: 16442636
Maternal oxytocin triggers a transient inhibitory switch in GABA signaling in the fetal brain during delivery.
Tyzio R, Cossart R, Khalilov I, Minlebaev M, Hubner CA, Represa A, Ben-Ari Y, Khazipov R., Science 314(5806), 2006
PMID: 17170309
Tyzio R, Cossart R, Khalilov I, Minlebaev M, Hubner CA, Represa A, Ben-Ari Y, Khazipov R., Science 314(5806), 2006
PMID: 17170309
Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium.
Dong CY, Koenig K, So P., J Biomed Opt 8(3), 2003
PMID: 12880351
Dong CY, Koenig K, So P., J Biomed Opt 8(3), 2003
PMID: 12880351
Spatially localized ballistic two-photon excitation in scattering media.
Szmacinski H, Gryczynski I, Lakowicz JR., Biospectroscopy 4(5), 1998
PMID: 9787906
Szmacinski H, Gryczynski I, Lakowicz JR., Biospectroscopy 4(5), 1998
PMID: 9787906
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
High-speed, two-photon scanning microscope.
Kim KH, Buehler C, So PT., Appl Opt 38(28), 1999
PMID: 18324120
Kim KH, Buehler C, So PT., Appl Opt 38(28), 1999
PMID: 18324120
Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes.
Roorda RD, Hohl TM, Toledo-Crow R, Miesenbock G., J. Neurophysiol. 92(1), 2004
PMID: 14999051
Roorda RD, Hohl TM, Toledo-Crow R, Miesenbock G., J. Neurophysiol. 92(1), 2004
PMID: 14999051
Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons.
Fan GY, Fujisaki H, Miyawaki A, Tsay RK, Tsien RY, Ellisman MH., Biophys. J. 76(5), 1999
PMID: 10233058
Fan GY, Fujisaki H, Miyawaki A, Tsay RK, Tsien RY, Ellisman MH., Biophys. J. 76(5), 1999
PMID: 10233058
Effect of a confocal pinhole in two-photon microscopy.
Gauderon R, Lukins PB, Sheppard CJ., Microsc. Res. Tech. 47(3), 1999
PMID: 10544336
Gauderon R, Lukins PB, Sheppard CJ., Microsc. Res. Tech. 47(3), 1999
PMID: 10544336
AUTHOR UNKNOWN, 0
AUTHOR UNKNOWN, 0
Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging.
Centonze VE, White JG., Biophys. J. 75(4), 1998
PMID: 9746543
Centonze VE, White JG., Biophys. J. 75(4), 1998
PMID: 9746543
Nonlinear magic: multiphoton microscopy in the biosciences.
Zipfel WR, Williams RM, Webb WW., Nat. Biotechnol. 21(11), 2003
PMID: 14595365
Zipfel WR, Williams RM, Webb WW., Nat. Biotechnol. 21(11), 2003
PMID: 14595365
Sheppard, J Opt Soc Am A 4(), 1987
AUTHOR UNKNOWN, 0
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