Imaging diffusion in living cells using time-correlated single-photon counting
Roth CM, Heinlein PI, Heilemann M, Herten D-P (2007)
ANALYTICAL CHEMISTRY 79(19): 7340-7345.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Roth, Christian M.;
Heinlein, Pia I.;
Heilemann, MikeUniBi;
Herten, Dirk-Peter
Einrichtung
Abstract / Bemerkung
Current efforts to monitor the diffusion of proteins in living cells are based on either fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching, or image correlation spectroscopy. However, these methods cannot generate a map of diffusion times. Here, we introduce a new method termed diffusion imaging microscopy that combines scanning confocal microscopy, time-correlated single-photon counting, and FCS and thus allows us to measure spatially resolved diffusion times. In our approach, we record scan images with time-resolved photon streams within each individual pixel. By extending the pixel dwell time to 25-100 ms, a software correlation of individual photons within each pixel yields the average diffusion time. Additionally, information on fluorescence intensity (number of photons) and fluorescence lifetime is available and can be used to sort fluorescence photons and to discriminate from autofluorescence. We evaluated our method by measuring diffusion times of dT(20)-TMR in solutions of different viscosity. We further demonstrate the applicability of the method to living cells and recorded a diffusion map of a living 3T3 mouse fibroblast incubated with dT(20)-ATIP0488.
Erscheinungsjahr
2007
Zeitschriftentitel
ANALYTICAL CHEMISTRY
Band
79
Ausgabe
19
Seite(n)
7340-7345
ISSN
0003-2700
eISSN
1520-6882
Page URI
https://pub.uni-bielefeld.de/record/1631858
Zitieren
Roth CM, Heinlein PI, Heilemann M, Herten D-P. Imaging diffusion in living cells using time-correlated single-photon counting. ANALYTICAL CHEMISTRY. 2007;79(19):7340-7345.
Roth, C. M., Heinlein, P. I., Heilemann, M., & Herten, D. - P. (2007). Imaging diffusion in living cells using time-correlated single-photon counting. ANALYTICAL CHEMISTRY, 79(19), 7340-7345. https://doi.org/10.1021/ac071039q
Roth, Christian M., Heinlein, Pia I., Heilemann, Mike, and Herten, Dirk-Peter. 2007. “Imaging diffusion in living cells using time-correlated single-photon counting”. ANALYTICAL CHEMISTRY 79 (19): 7340-7345.
Roth, C. M., Heinlein, P. I., Heilemann, M., and Herten, D. - P. (2007). Imaging diffusion in living cells using time-correlated single-photon counting. ANALYTICAL CHEMISTRY 79, 7340-7345.
Roth, C.M., et al., 2007. Imaging diffusion in living cells using time-correlated single-photon counting. ANALYTICAL CHEMISTRY, 79(19), p 7340-7345.
C.M. Roth, et al., “Imaging diffusion in living cells using time-correlated single-photon counting”, ANALYTICAL CHEMISTRY, vol. 79, 2007, pp. 7340-7345.
Roth, C.M., Heinlein, P.I., Heilemann, M., Herten, D.-P.: Imaging diffusion in living cells using time-correlated single-photon counting. ANALYTICAL CHEMISTRY. 79, 7340-7345 (2007).
Roth, Christian M., Heinlein, Pia I., Heilemann, Mike, and Herten, Dirk-Peter. “Imaging diffusion in living cells using time-correlated single-photon counting”. ANALYTICAL CHEMISTRY 79.19 (2007): 7340-7345.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
8 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Comparative advantages and limitations of the basic metrology methods applied to the characterization of nanomaterials.
Linkov P, Artemyev M, Efimov AE, Nabiev I., Nanoscale 5(19), 2013
PMID: 23934544
Linkov P, Artemyev M, Efimov AE, Nabiev I., Nanoscale 5(19), 2013
PMID: 23934544
Dissecting chromatin interactions in living cells from protein mobility maps.
Erdel F, Müller-Ott K, Baum M, Wachsmuth M, Rippe K., Chromosome Res 19(1), 2011
PMID: 20848178
Erdel F, Müller-Ott K, Baum M, Wachsmuth M, Rippe K., Chromosome Res 19(1), 2011
PMID: 20848178
Experimental approach to extend the range for counting fluorescent molecules based on photon-antibunching.
Ta H, Kiel A, Wahl M, Herten DP., Phys Chem Chem Phys 12(35), 2010
PMID: 20603676
Ta H, Kiel A, Wahl M, Herten DP., Phys Chem Chem Phys 12(35), 2010
PMID: 20603676
Measuring and imaging diffusion with multiple scan speed image correlation spectroscopy.
Gröner N, Capoulade J, Cremer C, Wachsmuth M., Opt Express 18(20), 2010
PMID: 20941019
Gröner N, Capoulade J, Cremer C, Wachsmuth M., Opt Express 18(20), 2010
PMID: 20941019
Multiparameter fluorescence image spectroscopy to study molecular interactions.
Weidtkamp-Peters S, Felekyan S, Bleckmann A, Simon R, Becker W, Kühnemuth R, Seidel CA., Photochem Photobiol Sci 8(4), 2009
PMID: 19337660
Weidtkamp-Peters S, Felekyan S, Bleckmann A, Simon R, Becker W, Kühnemuth R, Seidel CA., Photochem Photobiol Sci 8(4), 2009
PMID: 19337660
Mapping eGFP oligomer mobility in living cell nuclei.
Dross N, Spriet C, Zwerger M, Müller G, Waldeck W, Langowski J., PLoS One 4(4), 2009
PMID: 19347038
Dross N, Spriet C, Zwerger M, Müller G, Waldeck W, Langowski J., PLoS One 4(4), 2009
PMID: 19347038
Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI).
Dertinger T, Colyer R, Iyer G, Weiss S, Enderlein J., Proc Natl Acad Sci U S A 106(52), 2009
PMID: 20018714
Dertinger T, Colyer R, Iyer G, Weiss S, Enderlein J., Proc Natl Acad Sci U S A 106(52), 2009
PMID: 20018714
The F-techniques: advances in receptor protein studies.
Liu P, Ahmed S, Wohland T., Trends Endocrinol Metab 19(5), 2008
PMID: 18387308
Liu P, Ahmed S, Wohland T., Trends Endocrinol Metab 19(5), 2008
PMID: 18387308
References
Daten bereitgestellt von Europe PubMed Central.
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 17803281
PubMed | Europe PMC
Suchen in