Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis

Schulze P, Schüttpelz M, Sauer M, Belder D (2007)
LAB ON A CHIP 7(12): 1841-1844.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor/in
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Abstract / Bemerkung
Two photon excited (TPE) fluorescence detection was applied to native fluorescence detection of aromatics in microchip electrophoresis (MCE). This technique was evaluated as an alternative to common one photon excitation in the deep UV spectral range. TPE enables fluorescence detection of unlabeled aromatic compounds, even in non-deep UV-transparent microfluidic chips. In this study, we demonstrate the proof of concept of native TPE fluorescence detection of small aromatics in commercial microfluidic glass chips. Label-free TPE fluorescence detection of native proteins and small aromatics in MCE was achieved within the micromolar concentration range, utilising 420 nm excitation light.
Erscheinungsjahr
2007
Zeitschriftentitel
LAB ON A CHIP
Band
7
Ausgabe
12
Seite(n)
1841-1844
ISSN
1473-0197
eISSN
1473-0189
Page URI
https://pub.uni-bielefeld.de/record/1631388

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Schulze P, Schüttpelz M, Sauer M, Belder D. Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis. LAB ON A CHIP. 2007;7(12):1841-1844.
Schulze, P., Schüttpelz, M., Sauer, M., & Belder, D. (2007). Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis. LAB ON A CHIP, 7(12), 1841-1844. doi:10.1039/b710762e
Schulze, P., Schüttpelz, M., Sauer, M., and Belder, D. (2007). Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis. LAB ON A CHIP 7, 1841-1844.
Schulze, P., et al., 2007. Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis. LAB ON A CHIP, 7(12), p 1841-1844.
P. Schulze, et al., “Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis”, LAB ON A CHIP, vol. 7, 2007, pp. 1841-1844.
Schulze, P., Schüttpelz, M., Sauer, M., Belder, D.: Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis. LAB ON A CHIP. 7, 1841-1844 (2007).
Schulze, Philipp, Schüttpelz, Mark, Sauer, Markus, and Belder, Detlev. “Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis”. LAB ON A CHIP 7.12 (2007): 1841-1844.

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PMID: 18030410
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