Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering

Patkowski A, Eimer W, Dorfmüller T (1990)
Biopolymers 30(9-10): 975-983.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Patkowski, A.; Eimer, W.; Dorfmüller, Th.
Abstract / Bemerkung
The collective internal dynamics of transfer RNA(Phe) from brewer's yeast in solution was studied by depolarized dynamic light scattering (DDLS). Within the melting region of tRNA the depolarized spectra consist of two Lorentzian, where the narrow (slow) component described the overall rotation of the macromolecule. The broad component is attributed to the collective reorientation of the bases within the biopolymer. At high temperature only this relaxation process is observed in the spectrum. The viscosity dependence of the collective internal relaxation process is described by the Stokes-Einstein-Debye equation for the rotational diffusion. Estimates of the internal orientational pair correlation factor from the integral depolarized intensities of tRNA(Phe) solutions indicates that the observed dynamics correspond to the collective reorientation of approximately 5 bases. A comparison of the results presented with DDLS studies on the aggregation of the mononucleotide guanosine-process we studied the effect of hydrostatic pressure (1-1000 bar) on the depolarized spectra of tRNA. While other spectroscopic methods like nmr, fluorescence polarization anisotropy decay, or ESR give information about the very local motion of a single base within the DNA or RNA, this study shows that by DDLS one can characterize collective internal motions of macromolecules.
Erscheinungsjahr
1990
Zeitschriftentitel
Biopolymers
Band
30
Ausgabe
9-10
Seite(n)
975-983
ISSN
0006-3525
Page URI
https://pub.uni-bielefeld.de/record/1629894

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Patkowski A, Eimer W, Dorfmüller T. Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering. Biopolymers. 1990;30(9-10):975-983.
Patkowski, A., Eimer, W., & Dorfmüller, T. (1990). Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering. Biopolymers, 30(9-10), 975-983. https://doi.org/10.1002/bip.360300912
Patkowski, A., Eimer, W., and Dorfmüller, T. (1990). Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering. Biopolymers 30, 975-983.
Patkowski, A., Eimer, W., & Dorfmüller, T., 1990. Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering. Biopolymers, 30(9-10), p 975-983.
A. Patkowski, W. Eimer, and T. Dorfmüller, “Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering”, Biopolymers, vol. 30, 1990, pp. 975-983.
Patkowski, A., Eimer, W., Dorfmüller, T.: Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering. Biopolymers. 30, 975-983 (1990).
Patkowski, A., Eimer, W., and Dorfmüller, Th. “Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering”. Biopolymers 30.9-10 (1990): 975-983.

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