EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT
KURSCHAT P, GRAEVE L, ERREN A, GATSIOS P, ROSEJOHN S, ROEB E, Tschesche H, KOJ A, HEINRICH PC (1995)
EUROPEAN JOURNAL OF BIOCHEMISTRY 234(2): 485-491.
Zeitschriftenaufsatz
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Autor*in
KURSCHAT, P;
GRAEVE, L;
ERREN, A;
GATSIOS, P;
ROSEJOHN, S;
ROEB, E;
Tschesche, HaraldUniBi;
KOJ, A;
HEINRICH, PC
Einrichtung
Abstract / Bemerkung
Murine tissue inhibitor of metalloproteinases-1 (mTIMP-1) was expressed in baculovirus-infected insect cells (Sf9). The protein secreted into the culture medium was purified to homogeneity by means of heparin-Sepharose CL-6B and FPLC. The purified protein showed metalloproteinase-inhibitory activity in two independent assays: reverse zymography and inhibition of collagenase activity. Digestion of the recombinant TIMP-1 with peptide-N-glycanaseF revealed that both N-glycosylation sites are used. I-125-mTIMP-1 intraveneously injected into a male Sprague Dawley rat disappeared within 2 min from the circulation. 5 min after injection more than 50% of the I-125-mTIMP-1 were found in the liver and 20% in the kidneys. At later times, trichloroacetic-acid-soluble material accumulated in the intestinal tract.
Stichworte
TISSUE;
INHIBITOR OF METALLOPROTEINASE-1;
N-GLYCOSYLATION;
COLLAGENASE;
BACULOVIRUS;
METALLOPROTEINASES
Erscheinungsjahr
1995
Zeitschriftentitel
EUROPEAN JOURNAL OF BIOCHEMISTRY
Band
234
Ausgabe
2
Seite(n)
485-491
ISSN
0014-2956
eISSN
1432-1033
Page URI
https://pub.uni-bielefeld.de/record/1629264
Zitieren
KURSCHAT P, GRAEVE L, ERREN A, et al. EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1995;234(2):485-491.
KURSCHAT, P., GRAEVE, L., ERREN, A., GATSIOS, P., ROSEJOHN, S., ROEB, E., Tschesche, H., et al. (1995). EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY, 234(2), 485-491. https://doi.org/10.1111/j.1432-1033.1995.485_b.x
KURSCHAT, P, GRAEVE, L, ERREN, A, GATSIOS, P, ROSEJOHN, S, ROEB, E, Tschesche, Harald, KOJ, A, and HEINRICH, PC. 1995. “EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT”. EUROPEAN JOURNAL OF BIOCHEMISTRY 234 (2): 485-491.
KURSCHAT, P., GRAEVE, L., ERREN, A., GATSIOS, P., ROSEJOHN, S., ROEB, E., Tschesche, H., KOJ, A., and HEINRICH, P. C. (1995). EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY 234, 485-491.
KURSCHAT, P., et al., 1995. EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY, 234(2), p 485-491.
P. KURSCHAT, et al., “EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT”, EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 234, 1995, pp. 485-491.
KURSCHAT, P., GRAEVE, L., ERREN, A., GATSIOS, P., ROSEJOHN, S., ROEB, E., Tschesche, H., KOJ, A., HEINRICH, P.C.: EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY. 234, 485-491 (1995).
KURSCHAT, P, GRAEVE, L, ERREN, A, GATSIOS, P, ROSEJOHN, S, ROEB, E, Tschesche, Harald, KOJ, A, and HEINRICH, PC. “EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT”. EUROPEAN JOURNAL OF BIOCHEMISTRY 234.2 (1995): 485-491.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
6 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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