Actin-titin interaction in cardiac myofibrils: Probing a physiological role

Linke WA, Ivemeyer M, Labeit S, Hinssen H, Ruegg JC, Gautel M (1997)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Linke, WA; Ivemeyer, M; Labeit, S; Hinssen, HorstUniBi; Ruegg, JC; Gautel, M
Abstract / Bemerkung
The high stiffness of relaxed cardiac myofibrils is explainable mainly by the expression of a short-length titin (connectin), the giant elastic protein of the vertebrate myofibrillar cytoskeleton. However, additional molecular features could account for this high stiffness, such as interaction between titin and actin, which has previously been reported in vitro. To probe this finding for a possible physiological significance, isolated myofibrils from rat heart were subjected to selective removal of actin filaments by a calcium-independent gelsolin fragment, and the ''passive'' stiffness of the specimens was recorded. Upon actin extraction, stiffness decreased by nearly 60%, and to a similar degree after high-salt extraction of thick filaments. Thus actin-titin association indeed contributes to the stiffness of resting cardiac muscle. To identify possible sites of association, we employed a combination of different techniques. Immunofluorescence microscopy revealed that actin extraction increased the extensibility of the previously stiff Z-disc-flanking titin region. Actin-titin interaction within this region was confirmed in in vitro cosedimentation assays, in which multimodule recombinant titin fragments were tested for their ability to interact with F-actin. By contrast, such assays showed no actin-titin-binding propensity for sarcomeric regions outside the Z-disc comb. Accordingly, the results of mechanical measurements demonstrated that competition with native titin by recombinant titin fragments from Z-disc-remote, I-band or A-band regions did not affect passive myofibril stiffness. These results indicate that it is actin-titin association near the Z-disc, but not along the remainder of the sarcomere, that helps to anchor the titin molecule at its N-terminus and maintain a high stiffness of the relaxed cardiac myofibril.
Page URI


Linke WA, Ivemeyer M, Labeit S, Hinssen H, Ruegg JC, Gautel M. Actin-titin interaction in cardiac myofibrils: Probing a physiological role. BIOPHYSICAL JOURNAL. 1997;73(2):905-919.
Linke, W. A., Ivemeyer, M., Labeit, S., Hinssen, H., Ruegg, J. C., & Gautel, M. (1997). Actin-titin interaction in cardiac myofibrils: Probing a physiological role. BIOPHYSICAL JOURNAL, 73(2), 905-919.
Linke, WA, Ivemeyer, M, Labeit, S, Hinssen, Horst, Ruegg, JC, and Gautel, M. 1997. “Actin-titin interaction in cardiac myofibrils: Probing a physiological role”. BIOPHYSICAL JOURNAL 73 (2): 905-919.
Linke, W. A., Ivemeyer, M., Labeit, S., Hinssen, H., Ruegg, J. C., and Gautel, M. (1997). Actin-titin interaction in cardiac myofibrils: Probing a physiological role. BIOPHYSICAL JOURNAL 73, 905-919.
Linke, W.A., et al., 1997. Actin-titin interaction in cardiac myofibrils: Probing a physiological role. BIOPHYSICAL JOURNAL, 73(2), p 905-919.
W.A. Linke, et al., “Actin-titin interaction in cardiac myofibrils: Probing a physiological role”, BIOPHYSICAL JOURNAL, vol. 73, 1997, pp. 905-919.
Linke, W.A., Ivemeyer, M., Labeit, S., Hinssen, H., Ruegg, J.C., Gautel, M.: Actin-titin interaction in cardiac myofibrils: Probing a physiological role. BIOPHYSICAL JOURNAL. 73, 905-919 (1997).
Linke, WA, Ivemeyer, M, Labeit, S, Hinssen, Horst, Ruegg, JC, and Gautel, M. “Actin-titin interaction in cardiac myofibrils: Probing a physiological role”. BIOPHYSICAL JOURNAL 73.2 (1997): 905-919.

90 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Does partial titin degradation affect sarcomere length nonuniformities and force in active and passive myofibrils?
Joumaa V, Bertrand F, Liu S, Poscente S, Herzog W., Am J Physiol Cell Physiol 315(3), 2018
PMID: 29768046
Basic science and clinical use of eccentric contractions: History and uncertainties.
Nishikawa KC, Lindstedt SL, LaStayo PC., J Sport Health Sci 7(3), 2018
PMID: 30356648
Calcium increases titin N2A binding to F-actin and regulated thin filaments.
Dutta S, Tsiros C, Sundar SL, Athar H, Moore J, Nelson B, Gage MJ, Nishikawa K., Sci Rep 8(1), 2018
PMID: 30275509
α-Actinin/titin interaction: A dynamic and mechanically stable cluster of bonds in the muscle Z-disk.
Grison M, Merkel U, Kostan J, Djinović-Carugo K, Rief M., Proc Natl Acad Sci U S A 114(5), 2017
PMID: 28096424
Residual force enhancement is regulated by titin in skeletal and cardiac myofibrils.
Shalabi N, Cornachione A, de Souza Leite F, Vengallatore S, Rassier DE., J Physiol 595(6), 2017
PMID: 28028799
Nuances of electrophoresis study of titin/connectin.
Vikhlyantsev IM, Podlubnaya ZA., Biophys Rev 9(3), 2017
PMID: 28555301
Overview of the Muscle Cytoskeleton.
Henderson CA, Gomez CG, Novak SM, Mi-Mi L, Gregorio CC., Compr Physiol 7(3), 2017
PMID: 28640448
When signalling goes wrong: pathogenic variants in structural and signalling proteins causing cardiomyopathies.
Ehsan M, Jiang H, L Thomson K, Gehmlich K., J Muscle Res Cell Motil 38(3-4), 2017
PMID: 29119312
Differences in titin segmental elongation between passive and active stretch in skeletal muscle.
DuVall MM, Jinha A, Schappacher-Tilp G, Leonard TR, Herzog W., J Exp Biol 220(pt 23), 2017
PMID: 28970245
The increase in non-cross-bridge forces after stretch of activated striated muscle is related to titin isoforms.
Cornachione AS, Leite F, Bagni MA, Rassier DE., Am J Physiol Cell Physiol 310(1), 2016
PMID: 26405100
Non-crossbridge stiffness in active muscle fibres.
Colombini B, Nocella M, Bagni MA., J Exp Biol 219(pt 2), 2016
PMID: 26792325
Residual Force Enhancement Following Eccentric Contractions: A New Mechanism Involving Titin.
Herzog W, Schappacher G, DuVall M, Leonard TR, Herzog JA., Physiology (Bethesda) 31(4), 2016
PMID: 27252165
Non-crossbridge forces in activated striated muscles: a titin dependent mechanism of regulation?
Rassier DE, Leite FS, Nocella M, Cornachione AS, Colombini B, Bagni MA., J Muscle Res Cell Motil 36(1), 2015
PMID: 25421125
Syndecan-4 is a key determinant of collagen cross-linking and passive myocardial stiffness in the pressure-overloaded heart.
Herum KM, Lunde IG, Skrbic B, Louch WE, Hasic A, Boye S, Unger A, Brorson SH, Sjaastad I, Tønnessen T, Linke WA, Gomez MF, Christensen G., Cardiovasc Res 106(2), 2015
PMID: 25587045
Calcium sensitivity of residual force enhancement in rabbit skinned fibers.
Joumaa V, Herzog W., Am J Physiol Cell Physiol 307(4), 2014
PMID: 24965591
A rising titan: TTN review and mutation update.
Chauveau C, Rowell J, Ferreiro A., Hum Mutat 35(9), 2014
PMID: 24980681
Is titin a 'winding filament'? A new twist on muscle contraction.
Nishikawa KC, Monroy JA, Uyeno TE, Yeo SH, Pai DK, Lindstedt SL., Proc Biol Sci 279(1730), 2012
PMID: 21900329
Titin-based tension in the cardiac sarcomere: molecular origin and physiological adaptations.
Anderson BR, Granzier HL., Prog Biophys Mol Biol 110(2-3), 2012
PMID: 22910434
The sarcomeric cytoskeleton: who picks up the strain?
Gautel M., Curr Opin Cell Biol 23(1), 2011
PMID: 21190822
Titin based viscosity in ventricular physiology: an integrative investigation of PEVK-actin interactions.
Chung CS, Methawasin M, Nelson OL, Radke MH, Hidalgo CG, Gotthardt M, Granzier HL., J Mol Cell Cardiol 51(3), 2011
PMID: 21708170
Titin-actin interaction: PEVK-actin-based viscosity in a large animal.
Chung CS, Bogomolovas J, Gasch A, Hidalgo CG, Labeit S, Granzier HL., J Biomed Biotechnol 2011(), 2011
PMID: 22162634
Transcriptional and posttranslational modifications of titin: implications for diastole.
Borbély A, van Heerebeek L, Paulus WJ., Circ Res 104(1), 2009
PMID: 19118283
Hypophosphorylation of the Stiff N2B titin isoform raises cardiomyocyte resting tension in failing human myocardium.
Borbély A, Falcao-Pires I, van Heerebeek L, Hamdani N, Edes I, Gavina C, Leite-Moreira AF, Bronzwaer JG, Papp Z, van der Velden J, Stienen GJ, Paulus WJ., Circ Res 104(6), 2009
PMID: 19179657
Titin-based mechanical signalling in normal and failing myocardium.
Krüger M, Linke WA., J Mol Cell Cardiol 46(4), 2009
PMID: 19639676
Muscle giants: molecular scaffolds in sarcomerogenesis.
Kontrogianni-Konstantopoulos A, Ackermann MA, Bowman AL, Yap SV, Bloch RJ., Physiol Rev 89(4), 2009
PMID: 19789381
The origin of passive force enhancement in skeletal muscle.
Joumaa V, Rassier DE, Leonard TR, Herzog W., Am J Physiol Cell Physiol 294(1), 2008
PMID: 17928540
Evidence for the oligomeric state of 'elastic' titin in muscle sarcomeres.
Houmeida A, Baron A, Keen J, Khan GN, Knight PJ, Stafford WF, Thirumurugan K, Thompson B, Tskhovrebova L, Trinick J., J Mol Biol 384(2), 2008
PMID: 18824175
Sarcomere dynamics during muscular contraction and their implications to muscle function.
Telley IA, Denoth J., J Muscle Res Cell Motil 28(1), 2007
PMID: 17530424
The transitional junction: a new functional subcellular domain at the intercalated disc.
Bennett PM, Maggs AM, Baines AJ, Pinder JC., Mol Biol Cell 17(4), 2006
PMID: 16481394
Transverse stiffness of myofibrils of skeletal and cardiac muscles studied by atomic force microscopy.
Akiyama N, Ohnuki Y, Kunioka Y, Saeki Y, Yamada T., J Physiol Sci 56(2), 2006
PMID: 16839448
Effects of BDM, [Ca2+]o, and temperature on the dynamic stiffness of quiescent cardiac trabeculae from rat.
Kirton RS, Taberner AJ, Nielsen PM, Young AA, Loiselle DS., Am J Physiol Heart Circ Physiol 288(4), 2005
PMID: 15576440
Plasticity of cardiac titin/connectin in heart development.
Opitz CA, Linke WA., J Muscle Res Cell Motil 26(6-8), 2005
PMID: 16465471
Restoring force development by titin/connectin and assessment of Ig domain unfolding.
Preetha N, Yiming W, Helmes M, Norio F, Siegfried L, Granzier H., J Muscle Res Cell Motil 26(6-8), 2005
PMID: 16470334
Isoform diversity of giant proteins in relation to passive and active contractile properties of rabbit skeletal muscles.
Prado LG, Makarenko I, Andresen C, Krüger M, Opitz CA, Linke WA., J Gen Physiol 126(5), 2005
PMID: 16230467
Decline in titin content in rat skeletal muscle after denervation.
Chen SP, Sheu JR, Lin AC, Hsiao G, Fong TH., Muscle Nerve 32(6), 2005
PMID: 16175625
Strain softening is not present during axial extensions of rat intact right ventricular trabeculae in the presence or absence of 2,3-butanedione monoxime.
Kirton RS, Taberner AJ, Young AA, Nielsen PM, Loiselle DS., Am J Physiol Heart Circ Physiol 286(2), 2004
PMID: 14551051
Calcium transients regulate patterned actin assembly during myofibrillogenesis.
Li H, Cook JD, Terry M, Spitzer NC, Ferrari MB., Dev Dyn 229(2), 2004
PMID: 14745949
Developmentally regulated switching of titin size alters myofibrillar stiffness in the perinatal heart.
Opitz CA, Leake MC, Makarenko I, Benes V, Linke WA., Circ Res 94(7), 2004
PMID: 14988228
The elasticity of single titin molecules using a two-bead optical tweezers assay.
Leake MC, Wilson D, Gautel M, Simmons RM., Biophys J 87(2), 2004
PMID: 15298915
Multiple sources of passive stress relaxation in muscle fibres.
Linke WA, Leake MC., Phys Med Biol 49(16), 2004
PMID: 15446792
Excitation-contraction coupling in the heart and the negative inotropic action of volatile anesthetics.
Hanley PJ, ter Keurs HE, Cannell MB., Anesthesiology 101(4), 2004
PMID: 15448535
Regulation of the actin-myosin interaction by titin.
Niederländer N, Raynaud F, Astier C, Chaussepied P., Eur J Biochem 271(22), 2004
PMID: 15560799
Twitchin, a thick-filament protein from molluscan catch muscle, interacts with F-actin in a phosphorylation-dependent way.
Shelud'ko NS, Matusovskaya GG, Permyakova TV, Matusovsky OS., Arch Biochem Biophys 432(2), 2004
PMID: 15542066
Gigantic variety: expression patterns of titin isoforms in striated muscles and consequences for myofibrillar passive stiffness.
Neagoe C, Opitz CA, Makarenko I, Linke WA., J Muscle Res Cell Motil 24(2-3), 2003
PMID: 14609029
Alcohol affects the skeletal muscle proteins, titin and nebulin in male and female rats.
Hunter RJ, Neagoe C, Järveläinen HA, Martin CR, Lindros KO, Linke WA, Preedy VR., J Nutr 133(4), 2003
PMID: 12672935
Comparative electron microscopic study on projectin and titin binding to F-actin.
Podlubnaya ZA, Shpagina MD, Vikhlyantsev IM, Malyshev SL, Udaltsov SN, Ziegler C, Beinbrech G., Insect Biochem Mol Biol 33(8), 2003
PMID: 12878225
Damped elastic recoil of the titin spring in myofibrils of human myocardium.
Opitz CA, Kulke M, Leake MC, Neagoe C, Hinssen H, Hajjar RJ, Linke WA., Proc Natl Acad Sci U S A 100(22), 2003
PMID: 14563922
PEVK domain of titin: an entropic spring with actin-binding properties.
Linke WA, Kulke M, Li H, Fujita-Becker S, Neagoe C, Manstein DJ, Gautel M, Fernandez JM., J Struct Biol 137(1-2), 2002
PMID: 12064946
Titin as a modular spring: emerging mechanisms for elasticity control by titin in cardiac physiology and pathophysiology.
Granzier H, Labeit D, Wu Y, Labeit S., J Muscle Res Cell Motil 23(5-6), 2002
PMID: 12785097
Role of titin in vertebrate striated muscle.
Tskhovrebova L, Trinick J., Philos Trans R Soc Lond B Biol Sci 357(1418), 2002
PMID: 11911777
Titin-based contribution to shortening velocity of rabbit skeletal myofibrils.
Minajeva A, Neagoe C, Kulke M, Linke WA., J Physiol 540(pt 1), 2002
PMID: 11927678
Weakness of a giant: mutations of the sarcomeric protein titin.
Hein S, Schaper J., Trends Mol Med 8(7), 2002
PMID: 12114104
Titin and the sarcomere symmetry paradox.
Liversage AD, Holmes D, Knight PJ, Tskhovrebova L, Trinick J., J Mol Biol 305(3), 2001
PMID: 11152599
Unfolding of titin domains explains the viscoelastic behavior of skeletal myofibrils.
Minajeva A, Kulke M, Fernandez JM, Linke WA., Biophys J 80(3), 2001
PMID: 11222304
Kettin, a major source of myofibrillar stiffness in Drosophila indirect flight muscle.
Kulke M, Neagoe C, Kolmerer B, Minajeva A, Hinssen H, Bullard B, Linke WA., J Cell Biol 154(5), 2001
PMID: 11535621
Titin-actin interaction in mouse myocardium: passive tension modulation and its regulation by calcium/S100A1.
Yamasaki R, Berri M, Wu Y, Trombitás K, McNabb M, Kellermayer MS, Witt C, Labeit D, Labeit S, Greaser M, Granzier H., Biophys J 81(4), 2001
PMID: 11566799
Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein.
Weins A, Schwarz K, Faul C, Barisoni L, Linke WA, Mundel P., J Cell Biol 155(3), 2001
PMID: 11673475
Interaction between PEVK-titin and actin filaments: origin of a viscous force component in cardiac myofibrils.
Kulke M, Fujita-Becker S, Rostkova E, Neagoe C, Labeit D, Manstein DJ, Gautel M, Linke WA., Circ Res 89(10), 2001
PMID: 11701614
Targeting of cardiac muscle titin fragments to the Z-bands and dense bodies of living muscle and non-muscle cells.
Ayoob JC, Turnacioglu KK, Mittal B, Sanger JM, Sanger JW., Cell Motil Cytoskeleton 45(1), 2000
PMID: 10618168
Role of desmin filaments in chicken cardiac myofibrillogenesis.
Wang SM, Huang YS, Wu JC, Tseng YZ., J Cell Biochem 77(4), 2000
PMID: 10771519
The titin cDNA sequence and partial genomic sequences: insights into the molecular genetics, cell biology and physiology of the titin filament system.
Kolmerer B, Witt CC, Freiburg A, Millevoi S, Stier G, Sorimachi H, Pelin K, Carrier L, Schwartz K, Labeit D, Gregorio CC, Linke WA, Labeit S., Rev Physiol Biochem Pharmacol 138(), 1999
PMID: 10396137
The physiological role of titin in striated muscle.
Horowits R., Rev Physiol Biochem Pharmacol 138(), 1999
PMID: 10396138
Association of kettin with actin in the Z-disc of insect flight muscle.
van Straaten M, Goulding D, Kolmerer B, Labeit S, Clayton J, Leonard K, Bullard B., J Mol Biol 285(4), 1999
PMID: 9917396
Alpha actinin-CapZ, an anchoring complex for thin filaments in Z-line.
Papa I, Astier C, Kwiatek O, Raynaud F, Bonnal C, Lebart MC, Roustan C, Benyamin Y., J Muscle Res Cell Motil 20(2), 1999
PMID: 10412090
Muscle assembly: a titanic achievement?
Gregorio CC, Granzier H, Sorimachi H, Labeit S., Curr Opin Cell Biol 11(1), 1999
PMID: 10047523
3-Dimensional configuration of perimysial collagen fibres in rat cardiac muscle at resting and extended sarcomere lengths.
Hanley PJ, Young AA, LeGrice IJ, Edgar SG, Loiselle DS., J Physiol 517 ( Pt 3)(), 1999
PMID: 10358122
Modularity and homology: modelling of the type II module family from titin.
Fraternali F, Pastore A., J Mol Biol 290(2), 1999
PMID: 10390355
I-band titin in cardiac muscle is a three-element molecular spring and is critical for maintaining thin filament structure.
Linke WA, Rudy DE, Centner T, Gautel M, Witt C, Labeit S, Gregorio CC., J Cell Biol 146(3), 1999
PMID: 10444071
Defining actin filament length in striated muscle: rulers and caps or dynamic stability?
Littlefield R, Fowler VM., Annu Rev Cell Dev Biol 14(), 1998
PMID: 9891791
Striational autoantibodies in myasthenia gravis patients recognize I-band titin epitopes.
Lübke E, Freiburg A, Skeie GO, Kolmerer B, Labeit S, Aarli JA, Gilhus NE, Wollmann R, Wussling M, Rüegg JC, Linke WA., J Neuroimmunol 81(1-2), 1998
PMID: 9521611
Human autoantibodies reveal titin as a chromosomal protein.
Machado C, Sunkel CE, Andrew DJ., J Cell Biol 141(2), 1998
PMID: 9548712
Binding of a native titin fragment to actin is regulated by PIP2.
Astier C, Raynaud F, Lebart MC, Roustan C, Benyamin Y., FEBS Lett 429(1), 1998
PMID: 9657390
Nature of PEVK-titin elasticity in skeletal muscle.
Linke WA, Ivemeyer M, Mundel P, Stockmeier MR, Kolmerer B., Proc Natl Acad Sci U S A 95(14), 1998
PMID: 9653138
The NH2 terminus of titin spans the Z-disc: its interaction with a novel 19-kD ligand (T-cap) is required for sarcomeric integrity.
Gregorio CC, Trombitás K, Centner T, Kolmerer B, Stier G, Kunke K, Suzuki K, Obermayr F, Herrmann B, Granzier H, Sorimachi H, Labeit S., J Cell Biol 143(4), 1998
PMID: 9817758

63 References

Daten bereitgestellt von Europe PubMed Central.

The effect of 2,3-butanedione 2-monoxime (BDM) on ventricular trabeculae from the avian heart.
Brotto MA, Fogaca RT, Creazzo TL, Godt RE, Nosek TM., J. Muscle Res. Cell. Motil. 16(1), 1995
PMID: 7751400
Connectin, an elastic protein of muscle. Characterization and Function.
Maruyama K, Matsubara S, Natori R, Nonomura Y, Kimura S., J. Biochem. 82(2), 1977
PMID: 914784
Immunoglobulin-type domains of titin are stabilized by amino-terminal extension.
Politou AS, Gautel M, Joseph C, Pastore A., FEBS Lett. 352(1), 1994
PMID: 7925935
Passive and active tension in single cardiac myofibrils.
Linke WA, Popov VI, Pollack GH., Biophys. J. 67(2), 1994
PMID: 7948691
Elastic properties of connecting filaments along the sarcomere.
Trombitas K, Pollack GH., Adv. Exp. Med. Biol. 332(), 1993
PMID: 8109381
Localization and elasticity of connectin (titin) filaments in skinned frog muscle fibres subjected to partial depolymerization of thick filaments.
Higuchi H, Suzuki T, Kimura S, Yoshioka T, Maruyama K, Umazume Y., J. Muscle Res. Cell. Motil. 13(3), 1992
PMID: 1527215
Towards a molecular understanding of titin.
Labeit S, Gautel M, Lakey A, Trinick J., EMBO J. 11(5), 1992
PMID: 1582406
Regulation of skeletal muscle stiffness and elasticity by titin isoforms: a test of the segmental extension model of resting tension.
Wang K, McCarter R, Wright J, Beverly J, Ramirez-Mitchell R., Proc. Natl. Acad. Sci. U.S.A. 88(16), 1991
PMID: 1714586
Involvement of weak binding crossbridges in force production in muscle.
Chalovich JM, Yu LC, Brenner B., J. Muscle Res. Cell. Motil. 12(6), 1991
PMID: 1791190
Mechanical properties of isolated cardiac myocytes.
Brady AJ., Physiol. Rev. 71(2), 1991
PMID: 2006219
Gelsolin sensitivity of microfilaments as a marker for muscle differentiation.
Huckriede A, Hinssen H, Jockusch BM, Lazarides E., Eur. J. Cell Biol. 46(3), 1988
PMID: 2846307
Titin: major myofibrillar components of striated muscle.
Wang K, McClure J, Tu A., Proc. Natl. Acad. Sci. U.S.A. 76(8), 1979
PMID: 291034
Stiffness and shortening changes in myofilament-extracted rat cardiac myocytes.
Roos KP, Brady AJ., Am. J. Physiol. 256(2 Pt 2), 1989
PMID: 2916687
Stiffness of skinned rabbit psoas fibers in MgATP and MgPPi solution.
Brenner B, Chalovich JM, Greene LE, Eisenberg E, Schoenberg M., Biophys. J. 50(4), 1986
PMID: 3022835
Differential response of stress fibers and myofibrils to gelsolin.
Sanger JM, Mittal B, Wegner A, Jockusch BM, Sanger JW., Eur. J. Cell Biol. 43(3), 1987
PMID: 3040411
Binding of actin filaments to connectin.
Maruyama K, Hu DH, Suzuki T, Kimura S., J. Biochem. 101(6), 1987
PMID: 3667550
A physiological role for titin and nebulin in skeletal muscle.
Horowits R, Kempner ES, Bisher ME, Podolsky RJ., Nature 323(6084), 1986
PMID: 3755803
Active state in cardiac muscle.
Brady AJ., Physiol. Rev. 48(3), 1968
PMID: 4873866
A Ca2+-dependent actin modulator from vertebrate smooth muscle.
Hinssen H, Small JV, Sobieszek A., FEBS Lett. 166(1), 1984
PMID: 6537923
The mechanically active domain of titin in cardiac muscle.
Trombitas K, Jin JP, Granzier H., Circ. Res. 77(4), 1995
PMID: 7554133
Titins: giant proteins in charge of muscle ultrastructure and elasticity.
Labeit S, Kolmerer B., Science 270(5234), 1995
PMID: 7569978
Exogenous gelsolin binds to sarcomeric thin filaments without severing.
Gonsior S, Hinssen H., Cell Motil. Cytoskeleton 31(3), 1995
PMID: 7585989
Cardiac myocyte stiffness following extraction with detergent and high salt solutions.
Brady AJ, Farnsworth SP., Am. J. Physiol. 250(6 Pt 2), 1986
PMID: 3521334
Active tension generation in isolated skeletal myofibrils.
Bartoo ML, Popov VI, Fearn LA, Pollack GH., J. Muscle Res. Cell. Motil. 14(5), 1993
PMID: 8300845
An optical fiber transducer for single myofibril force measurement.
Fearn LA, Bartoo ML, Myers JA, Pollack GH., IEEE Trans Biomed Eng 40(11), 1993
PMID: 8307596
Elastic filaments in situ in cardiac muscle: deep-etch replica analysis in combination with selective removal of actin and myosin filaments.
Funatsu T, Kono E, Higuchi H, Kimura S, Ishiwata S, Yoshioka T, Maruyama K, Tsukita S., J. Cell Biol. 120(3), 1993
PMID: 8425898
A survey of interactions made by the giant protein titin.
Soteriou A, Gamage M, Trinick J., J. Cell. Sci. 104 ( Pt 1)(), 1993
PMID: 8449991
Viscoelasticity of the sarcomere matrix of skeletal muscles. The titin-myosin composite filament is a dual-stage molecular spring.
Wang K, McCarter R, Wright J, Beverly J, Ramirez-Mitchell R., Biophys. J. 64(4), 1993
PMID: 8494977
Nebulette: a 107 kD nebulin-like protein in cardiac muscle.
Moncman CL, Wang K., Cell Motil. Cytoskeleton 32(3), 1995
PMID: 8581976
Calcium-dependent inhibition of in vitro thin-filament motility by native titin.
Kellermayer MS, Granzier HL., FEBS Lett. 380(3), 1996
PMID: 8601441
A 11.5-kb 5'-terminal cDNA sequence of chicken breast muscle connectin/titin reveals its Z line binding region.
Yajima H, Ohtsuka H, Kawamura Y, Kume H, Murayama T, Abe H, Kimura S, Maruyama K., Biochem. Biophys. Res. Commun. 223(1), 1996
PMID: 8660363
Towards a molecular understanding of the elasticity of titin.
Linke WA, Ivemeyer M, Olivieri N, Kolmerer B, Ruegg JC, Labeit S., J. Mol. Biol. 261(1), 1996
PMID: 8760502
Immunoglobulin-like modules from titin I-band: extensible components of muscle elasticity.
Improta S, Politou AS, Pastore A., Structure 4(3), 1996
PMID: 8805538
Limits of titin extension in single cardiac myofibrils.
Linke WA, Bartoo ML, Ivemeyer M, Pollack GH., J. Muscle Res. Cell. Motil. 17(4), 1996
PMID: 8884598
Mechanical characterization of skeletal muscle myofibrils.
Friedman AL, Goldman YE., Biophys. J. 71(5), 1996
PMID: 8913614
The central Z-disk region of titin is assembled from a novel repeat in variable copy numbers.
Gautel M, Goulding D, Bullard B, Weber K, Furst DO., J. Cell. Sci. 109 ( Pt 11)(), 1996
PMID: 8937992
The giant protein titin. Emerging roles in physiology and pathophysiology.
Labeit S, Kolmerer B, Linke WA., Circ. Res. 80(2), 1997
PMID: 9012751

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

PMID: 9251807
PubMed | Europe PMC

Suchen in

Google Scholar