Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells

Maximov AV, Vedernikova EA, Hinssen H, Khaitlina SY, Negulyaev YA (1997)
FEBS LETTERS 412(1): 94-96.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ;
Abstract / Bemerkung
With the use of the patch-clamp technique, physiological mechanisms of Na+ channel regulation involving submembranous actin rearrangements were examined in human myeloid leukemia K562 cells. We found that the actin-severing protein gelsolin applied to cytoplasmic surface of membrane fragments at a high level of [Ca2+](i) (1 mu M) increased drastically the activity of Na-selective channels of 12 pS unitary conductance. In the experiments on intact cells, the elevation of [Ca2+](i) using the ionophore 4Br-A23187 also resulted in Na+ channel activation. Addition of actin to the cytoplasmic surface of membrane patches reduced this activity to background level, likely due to actin polymerization. Our data imply that Ca-dependent modulations of the actin cytoskeleton may represent one of the general mechanisms of channel regulation and cell signalling. (C) 1997 Federation of European Biochemical Societies.
Stichworte
cytoskeleton; Na+ channel; actin; gelsolin; leukemia cell; Ca regulation
Erscheinungsjahr
1997
Zeitschriftentitel
FEBS LETTERS
Band
412
Ausgabe
1
Seite(n)
94-96
ISSN
0014-5793
Page URI
https://pub.uni-bielefeld.de/record/1627757

Zitieren

Maximov AV, Vedernikova EA, Hinssen H, Khaitlina SY, Negulyaev YA. Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS. 1997;412(1):94-96.
Maximov, A. V., Vedernikova, E. A., Hinssen, H., Khaitlina, S. Y., & Negulyaev, Y. A. (1997). Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS, 412(1), 94-96. doi:10.1016/S0014-5793(97)00754-0
Maximov, A. V., Vedernikova, E. A., Hinssen, H., Khaitlina, S. Y., and Negulyaev, Y. A. (1997). Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS 412, 94-96.
Maximov, A.V., et al., 1997. Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS, 412(1), p 94-96.
A.V. Maximov, et al., “Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells”, FEBS LETTERS, vol. 412, 1997, pp. 94-96.
Maximov, A.V., Vedernikova, E.A., Hinssen, H., Khaitlina, S.Y., Negulyaev, Y.A.: Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS. 412, 94-96 (1997).
Maximov, AV, Vedernikova, EA, Hinssen, Horst, Khaitlina, SY, and Negulyaev, YA. “Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells”. FEBS LETTERS 412.1 (1997): 94-96.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 9257697
PubMed | Europe PMC

Suchen in

Google Scholar